Control of bacterial stem rot on tomato by extracellular bioactive compounds produced by <i>Pseudomonas aeruginosa</i> LV strain

Munhoz, Luana Delgado; Fonteque, Juliana Pistore; Santos, Igor Matheus Oliveira dos; Navarro, Miguel Octavio Pérez; Simionato, Ane Stéfano; Goya, Erika Tiemi; Rezende, Maria Inês; Balbi-Peña, María Isabel; Oliveira, Admilton Gonçalves de; Andrade, Galdino

doi:10.1080/23311932.2017.1282592

Cited by 22 publications

(16 citation statements)

References 28 publications

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“…However, effects of elicitors produced by these PGPRs have been less studied. These substances have been reported to be either structural molecules, such as flagellin (Ramirez-Prado et al 2018), or metabolic elicitors released to the medium (Munhoz et al 2017; Wu et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

et al. 2019

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We evaluated the ability of metabolic elicitors extracted from Pseudomonas fluorescens N21.4 to induce systemic resistance (ISR) in Arabidopsis thaliana against the pathogen Pseudomonas syringae DC3000. Metabolic elicitors were obtained from bacteria-free culture medium with n-hexane, ethyl acetate and n-butanol in three consecutive extractions. Each extract showed plant protection activity. The n-hexane fraction was the most effective and was used to study the signal transduction pathways involved by evaluating expression of marker genes of the salicylic acid (SA) signalling pathway (NPR1, PR1, ICS and PR2) and the jasmonic acid/ethylene (JA/ET) signalling pathway (PDF1, MYC2, LOX2 and PR3). In addition, the level of oxidative stress was tested by determining the activity of enzymes related to the ascorbate-glutathione cycle. N-hexane extracts stimulated both pathways based on overexpression of ICS, PR1, PR2, PDF1 and LOX2 genes. In addition, activity of the pathogenesis-related proteins glucanase (PR2) and chitinase (PR3), lipoxygenase and polyphenol oxidase was enhanced together with an increased capacity to remove reactive oxygen species (ROS). This was associated with less oxidative stress as indicated by a decrease in malondialdehyde (MDA), suggesting a causative link between defensive metabolism against P. syringae and ROS scavenging.

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Section: Discussionmentioning

confidence: 99%

Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

et al. 2019

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“…The development of molecules complexed with metals as potential medicinal agents has been rising the last decade (Cardozo et al, 2013; de Oliveira et al, 2016; Kerbauy et al, 2016; Munhoz et al, 2017; Stringer et al, 2017). Previous studies demonstrated that specific fractions obtained from the culture of Pseudomonas aeruginosa LV strain (F3, F3D, and F4A) present very strong antimicrobial activity against many different pathogens (de Oliveira et al, 2011, 2016; Cardozo et al, 2013; Murate et al, 2015; Kerbauy et al, 2016; Munhoz et al, 2017; Simionato et al, 2017). Indeed, our research group demonstrated the strong antibiotic activity of these semi-purified fractions against planktonic cells and biofilm formation of MDR isolates (Kerbauy et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

et al. 2019

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The increasing emergence of multidrug-resistant (MDR) organisms in hospital infections is causing a global public health crisis. The development of drugs with effective antibiotic action against such agents is of the highest priority. In the present study, the action of Fluopsin C against MDR clinical isolates was evaluated under in vitro and in vivo conditions. Fluopsin C was produced in cell suspension culture of Pseudomonas aeruginosa LV strain, purified by liquid adsorption chromatography and identified by mass spectrometric analysis. Bioactivity, bacterial resistance development risk against clinically important pathogenic strains and toxicity in mammalian cell were initially determined by in vitro models. In vivo toxicity was evaluated in Tenebrio molitor larvae and mice. The therapeutic efficacy of intravenous Fluopsin C administration was evaluated in a murine model of Klebsiella pneumoniae (KPC) acute sepsis, using six different treatments. The in vitro results indicated MIC and MBC below 2 μg/mL and low bacterial resistance development frequency. Electron microscopy showed that Fluopsin C may have altered the exopolysaccharide matrix and caused disruption of the cell wall of MDR bacteria. Best therapeutic results were achieved in mice treated with a single dose of 2 mg/kg and in mice treated with two doses of 1 mg/kg, 8 h apart. Furthermore, acute and chronic histopathological studies demonstrated absent nephrotoxicity and moderate hepatotoxicity. The results demonstrated the efficacy of Fluopsin C against MDR organisms in in vitro and in vivo models, and hence it can be a novel therapeutic agent for the control of severe MDR infections.

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“…Based on gene expression and protection results, the Gram-negative Serratia rubidaea N 12.34 and the Gram-positive Bacillus cereus N 4.1 were selected to extract their metabolic elicitors. Bacterial elicitors capable of starting defensive immune responses in plants, have been found to be structural molecules, (e.g., flagellin [53]), or metabolic elicitors that are released into the medium [17,45,[54][55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Bioeffectors as Biotechnological Tools to Boost Plant Innate Immunity: Signal Transduction Pathways Involved

Martin‐Rivilla

García‐Villaraco

Ramos‐Solano

et al. 2020

Plants

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The use of beneficial rhizobacteria (bioeffectors) and their derived metabolic elicitors are efficient biotechnological alternatives in plant immune system elicitation. This work aimed to check the ability of 25 bacterial strains isolated from the rhizosphere of Nicotiana glauca, and selected for their biochemical traits from a group of 175, to trigger the innate immune system of Arabidopsis thaliana seedlings against the pathogen Pseudomonas syringae pv. tomato DC3000. The five strains more effective in preventing pathogen infection were used to elucidate signal transduction pathways involved in the plant immune response by studying the differential expression of Salicylic acid and Jasmonic acid/Ethylene pathway marker genes. Some strains stimulated both pathways, while others stimulated either one or the other. The metabolic elicitors of two strains, chosen for the differential expression results of the genes studied, were extracted using n-hexane, ethyl acetate, and n-butanol, and their capacity to mimic bacterial effect to trigger the plant immune system was studied. N-hexane and ethyl acetate were the most effective fractions against the pathogen in both strains, achieving similar protection rates although gene expression responses were different from that obtained by the bacteria. These results open an amount of biotechnological possibilities to develop biological products for agriculture.

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Control of bacterial stem rot on tomato by extracellular bioactive compounds produced by Pseudomonas aeruginosa LV strain

Cited by 22 publications

References 28 publications

Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas syringae

Fluopsin C for Treating Multidrug-Resistant Infections: In vitro Activity Against Clinically Important Strains and in vivo Efficacy Against Carbapenemase-Producing Klebsiella pneumoniae

Bioeffectors as Biotechnological Tools to Boost Plant Innate Immunity: Signal Transduction Pathways Involved

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