Control of Bacteriophage Mu Lysogenic Repression

Ranquet, Caroline; Toussaint, Ariane; Jong, Hidde de; Maenhaut-Michel, G.; Geiselmann, Johannes

doi:10.1016/j.jmb.2005.08.015

Cited by 30 publications

(22 citation statements)

References 40 publications

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“…In a recent study of S. pyogenes, phage-related genes were not significantly up-expressed as cells entered the stationary phase, and many genes were down-expressed (7). Recently, the lytic cycle of the Mu phage in E. coli was shown to be derepressed via the action of ClpXP and Lon proteases on the Rep repressor, and ClpXP and the Lon protease were up-expressed during the transition to the stationary phase in E. coli (41). The data indicated that phage-related genes were up-expressed in response to a decrease in the level of the carbon and energy source and/or in response to a decreased growth rate of the host cells.…”

Section: Discussionmentioning

confidence: 99%

Temporal Transcriptomic Analysis as Desulfovibrio vulgaris Hildenborough Transitions into Stationary Phase during Electron Donor Depletion

Clark

et al. 2006

Appl Environ Microbiol

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Desulfovibrio vulgaris was cultivated in a defined medium, and biomass was sampled for approximately 70 h to characterize the shifts in gene expression as cells transitioned from the exponential to the stationary phase during electron donor depletion. In addition to temporal transcriptomics, total protein, carbohydrate, lactate, acetate, and sulfate levels were measured. The microarray data were examined for statistically significant expression changes, hierarchical cluster analysis, and promoter element prediction and were validated by quantitative PCR. As the cells transitioned from the exponential phase to the stationary phase, a majority of the down-expressed genes were involved in translation and transcription, and this trend continued at the remaining times. There were general increases in relative expression for intracellular trafficking and secretion, ion transport, and coenzyme metabolism as the cells entered the stationary phase. As expected, the DNA replication machinery was down-expressed, and the expression of genes involved in DNA repair increased during the stationary phase. Genes involved in amino acid acquisition, carbohydrate metabolism, energy production, and cell envelope biogenesis did not exhibit uniform transcriptional responses. Interestingly, most phage-related genes were up-expressed at the onset of the stationary phase. This result suggested that nutrient depletion may affect community dynamics and DNA transfer mechanisms of sulfate-reducing bacteria via the phage cycle. The putative feoAB system (in addition to other presumptive iron metabolism genes) was significantly up-expressed, and this suggested the possible importance of Fe 2؉ acquisition under metal-reducing conditions. The expression of a large subset of carbohydrate-related genes was altered, and the total cellular carbohydrate levels declined during the growth phase transition. Interestingly, the D. vulgaris genome does not contain a putative rpoS gene, a common attribute of the ␦-Proteobacteria genomes sequenced to date, and the transcription profiles of other putative rpo genes were not significantly altered. Our results indicated that in addition to expected changes (e.g., energy conversion, protein turnover, translation, transcription, and DNA replication and repair), genes related to phage, stress response, carbohydrate flux, the outer envelope, and iron homeostasis played important roles as D. vulgaris cells experienced electron donor depletion.The underground corrosion of metal pipes used for gas or water and the generation of sulfide during digestion of domestic and agricultural wastes have been the economic and environmental processes that have historically driven the desire to understand the metabolism of sulfate-reducing bacteria (SRB) (16). The SRB have been a particular problem for the petroleum industry due to their roles in metal corrosion, petroleum souring, and the health hazards of hydrogen sulfide. In contrast, SRB can be advantageous for bioremediation processes. A variety of studies (6,15,17,33,39) have...

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Section: Discussionmentioning

confidence: 99%

Temporal Transcriptomic Analysis as Desulfovibrio vulgaris Hildenborough Transitions into Stationary Phase during Electron Donor Depletion

Clark

et al. 2006

Appl Environ Microbiol

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“…Alternatively, a population of lysogenic cells may be induced to lyse by subjecting them to stress such as treatment with mutagenic agents or exposure to ultraviolet light. Some temperate phages such as Mu can switch between lysogeny and lytic growth under the influence of high temperature and stationary phase [10]. The prophage directs the synthesis of a repressor protein that blocks the transcription of its own genes and also those of closely related bacteriophages.…”

Section: Bacteriophage Biology and Interactions With Host Bacteriamentioning

confidence: 99%

Bacteriophages: an appraisal of their role in the treatment of bacterial infections

Hanlon

2007

International Journal of Antimicrobial Agents

308

187

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“…Further evidence for this linkage includes the spontaneous induction of prophage during biofilm development (15,23,57), the upregulation of phage-related genes in Desulfovibro vulgaris during stationary phase, and the induction of prophage Mu in stationary phase (11,40). In other instances, the spontaneous induction of prophage has been observed as cultures enter stationary phase and conditions associated with high cell density (10,12,40,52,57), situations in which quorum-sensing compounds for some bacteria might reach threshold concentrations that are necessary for the induction of cell-density-dependent processes.…”

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confidence: 99%

Acyl-Homoserine Lactones Can Induce Virus Production in Lysogenic Bacteria: an Alternative Paradigm for Prophage Induction

Ghosh

Roy

Williamson

et al. 2009

Appl Environ Microbiol

112

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Prophage typically are induced to a lytic cycle under stressful environmental conditions or when the host's survival is threatened. However, stress-independent, spontaneous induction also occurs in nature and may be cell density dependent, but the in vivo signal(s) that can trigger induction is unknown. In the present study, we report that acyl-homoserine lactones (AHL), the essential signaling molecules of quorum sensing in many gram-negative bacteria, can trigger phage production in soil and groundwater bacteria. This phenomenon also was operative in a lysogen of Escherichia coli. In model coculture systems, we monitored the real-time AHL production from Pseudomonas aeruginosa PAO1 using an AHL bioluminescent sensor and demonstrated that -prophage induction in E. coli was correlated with AHL production. As a working model in E. coli, we show that the induction responses of with AHL remained unaffected when recA was deleted, suggesting that this mechanism does not involve an SOS response. In the same lysogen we also demonstrated that sdiA, the AHL receptor, and rcsA, a positive transcriptional regulator of exopolysaccharide synthesis, are involved in the AHL-mediated induction process. These findings relate viral reproduction to chemical signals associated with high host cell abundance, suggesting an alternative paradigm for prophage induction.

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Control of Bacteriophage Mu Lysogenic Repression

Cited by 30 publications

References 40 publications

Temporal Transcriptomic Analysis as Desulfovibrio vulgaris Hildenborough Transitions into Stationary Phase during Electron Donor Depletion

Temporal Transcriptomic Analysis as Desulfovibrio vulgaris Hildenborough Transitions into Stationary Phase during Electron Donor Depletion

Bacteriophages: an appraisal of their role in the treatment of bacterial infections

Acyl-Homoserine Lactones Can Induce Virus Production in Lysogenic Bacteria: an Alternative Paradigm for Prophage Induction

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