Control of bovine leukaemia virus transmission by selective culling of infected cattle on the basis of viral antigen expression in lymphocyte cultures

Molloy, J.B.; Dimmock, Corinne K.; Eaves, F. W.; Bruyeres, Anthea G.; Cowley, Jeff A.; Ward, W.H.

doi:10.1016/0378-1135(94)90168-6

Cited by 16 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This pilot intervention field trial appears to be the first time that selective culling and segregation of the cattle likely to be most infectious, along with other management practices, has been used to reduce BLV transmission since 1994 in Australia (Molloy et al, 1994). Despite differences in herd size and management, all 3 herds experienced decreased within-herd BLV prevalence over the course of our study.…”

Section: Discussionmentioning

confidence: 80%

“…This is supported by studies in both Japan (Mekata et al, 2015) and Argentina (Juliarena et al, 2016), which found that cows with a low PVL were unlikely to spread BLV to herd mates. As well, removal of cattle with high BLV antigen expression reduced the incidence of new BLV infections in an Australian dairy herd (Molloy et al, 1994), and Gutiérrez et al (2014) reported that dams with high PVL were more likely to transmit BLV to their calves.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Controlling bovine leukemia virus in dairy herds by identifying and removing cows with the highest proviral load and lymphocyte counts

Ruggiero

Norby

Benitez

et al. 2019

Journal of Dairy Science

View full text Add to dashboard Cite

The objective of this field trial was to reduce bovine leukemia virus (BLV) transmission and prevalence in commercial dairy herds using proviral load (PVL) and lymphocyte count (LC) measurements as indicators of the most infectious animals for culling or segregation. Bovine leukemia virus causes lymphoma in <5% of infected cattle, and increased lymphocyte counts (lymphocytosis) in about one-third. Recent research has shown that dairy cows infected with BLV have altered immune function associated with decreases in milk production and lifespan. Recent findings show that a minority of infected cattle have PVL concentrations in blood and other body fluids of over 1,000 times that of other infected cattle. In combination with a high LC, these animals are thought to be responsible for most transmission of BLV in a herd. Milk or blood samples from adult cows in our 3 Midwestern dairy farm field trials were tested semiannually with ELISA for BLV antibodies, and ELISA-positive cattle were then retested using a blood LC and a quantitative PCR test for PVL to identify the animals presumed to be most infectious. Herd managers were encouraged to consider PVL and LC status when making cull decisions, and to segregate cows with the highest PVL and LC from their BLV ELISA-negative herd mates where possible. After 2 to 2.5 yr of this intervention, the incidence risk of new infections decreased in all 3 herds combined, from 13.8 to 2.2, and the overall herd prevalence decreased in all 3 herds combined from 62.0 to 20.7%, suggesting that this approach can efficiently reduce BLV transmission as well as prevalence. This is encouraging, because a very low prevalence of BLV infection would make it economically feasible to cull the remaining ELISA-positive cattle, as was achieved in national eradication programs in other countries decades ago.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 97%

Controlling bovine leukemia virus in dairy herds by identifying and removing cows with the highest proviral load and lymphocyte counts

Ruggiero

Norby

Benitez

et al. 2019

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Neither AGID nor ELISA discriminates maternal passive antibodies from an active immune response, and do not provide evidence of infection in its early stage (Ballagi-Pordány et al, 1992). Detection of specific antibodies to BLV by AGID or ELISA is not always sensitive enough to detect all BLV-infected cattle (Eaves et al, 1994;Klintevall et al, 1994).…”

Section: Detection Of Antibodies Against Blvmentioning

confidence: 99%

“…Over the last two decades PCR methods to detect proviral DNA of BLV have been developed. The majority of assays are based on a single PCR (Naif et al, 1990;Jacobs et al, 1992;Agresti et al, 1993;Kelly et al, 1993;Eaves et al, 1994); however, semi-nested (Mirsky et al, 1993) or the nested PCR provide a much higher sensitivity compared to single PCR. Modifications known as PCR-ELISA (Rola and Kuzmak, 2002) and PCR-EIA (Naif et al, 1992) have also been applied to detect proviral DNA.…”

Section: Detection Of Blv Provirusmentioning

confidence: 99%

Enzootic bovine leukosis

Sihvonen¹

2015

EFS2

View full text Add to dashboard Cite

Enzootic bovine leukosis is a disease of cattle caused by bovine leukemia virus (BLV). The virus causes a persistent, life‐long infection in a subset of B cells. Malignant tumours (lymphomas) ultimately develop in 2–5% of infected animals, predominantly in adult cattle older than 3–5 years. Lymphomas invariably lead to death of the animal within months. Before 1960, BLV was endemic in dairy herds in Northern/Eastern Europe and North America. Since then it has spread to all continents. The disease has been successfully controlled and eliminated from many countries in Europe. There is no evidence to suggest that any significant reservoir of BLV exists among other species, nor for any role of BLV in human disease or cancers. BLV exhibits a slow, progressive spread within a herd and it is likely to persist if control measures are not applied. The main modes of transmission are perinatal from cow to calf, via colostrum and milk, and close contact that allows transfer of infected lymphocytes, such as dehorning and injections using non‐sterile utensils. Transfer between herds is almost entirely by movement of infected animals. Suitable methods have been developed for diagnosis of BLV infection in specimens of blood, milk and lymphomas. EBL has a negative impact on milk yield and leads to increased premature culling. The welfare consequences of lymphomas vary according to the location and magnitude of organ involvement. Criteria for maintaining country freedom from EBL differ substantially between OIE and EU. Surveillance for freedom should be based on a combination of serological testing of adult animals and identification of lymphomas at slaughter.

show abstract

“…Não obstante, a maior parte dos animais com LEB permanece assintomática, alinfocitótica ou manifestando LP, atuando como reservatório e disseminando o vírus. Assim, esforços devem ser concentrados no controle da afecção (YOSHIKAWA et al, 1992;CORDEIRO et al, 1994;MOLLOY et al, 1994;DEREN;SZEWCZYK-SADOWSKA;RULKA, 2003).…”

Section: Introductionunclassified

Influência do vírus da lecucose bovina na resposta imunitária de animais naturalmente infectados

Azedo¹

View full text Add to dashboard Cite

No início de 2003, resolvi retornar a São Paulo para ter a oportunidade de ficar mais próximo de meus pais, à época, já com idade. A experiência dos 13 anos na Amazônia como, principalmente, buiatra, não seria inerente na busca por uma recolocação nesta cidade... e eu estava ciente disto. Mas, em mim, já havia surgido a vocação para a docência... uma possibilidade. No entanto, seria necessária a busca por aperfeiçoamento, aprender a ensinar... a responder e a perguntar. Seriam prementes Mestrado e Doutorado. Finda mais esta etapa, certo é que, a cada dia, ainda há muito que aprender, mas a vocação que surgira tornou-se paixão... Muitos foram fundamentais para que estes últimos anos fossem agradáveis e produtivos. Em especial, agradeço: À minha querida Amiga, Irmã e O Or ri ie en nt ta ad do or ra a, , P Pr ro of f a a D Dr r a a A Al li ic ce e M Ma ar ri ia a M Me el lv vi il ll le e P Pa ai iv va a D De el ll la a L Li ib be er ra a, , para sempre "m mi in nh ha a c ch he ef fi in nh ha a". Essencial... especial. Pelas conversas e conselhos, pelos ensinamentos, pela paciência, pela confiança, pelos exemplos de profissional prestativa e dedicada, de mãe, filha e esposa, sou eterna e incondicionalmente grato. À valiosa equipe formada pelos Médicos Veterinários Pós-Graduandos M Ma ai ia ar ra a G Ga ar rc ci ia a B Bl la ag gi it tz z, , C Ca am mi il la a F Fr re ei it ta as s B Ba at ti is st ta a, , T Ta at ti ia an na a d de e R Re ez ze en nd de e S Sp pí ín no ol la a, , M Me el li is ss sa a H Ha ar rt tm ma an n, , C Cl la au ud di ia a P Pe es st ta an na a R Ri ib be ei ir ro o, , B Bá ár rb ba ar ra a G Ga ab br ri ie el la a S So oa ar re es s S Sa an nc ch he es s e e F Fe er rn na an nd do o N No og gu ue ei ir ra a d de e S So ou uz za a. Pessoas tão heterogêneas e tão intensas e dedicadas. Nossas pesquisas, nossos trabalhos são fruto do esforço de cada um de nós. Nosso fim... o desenvolvimento de todos. Obrigado por tudo! Aos diletos Professores Doutores do Departamento de Clínica Médica, A Ar rc ch hi iv va al ld do o R Re ec ch he e J Jú ún ni io or r, , C Ca ar rl la a B Ba ar rg gi i B Be el ll li i, , C Ca ar rl lo os s E Ed du ua ar rd do o L La ar rs ss so on n, , C Cá ás ss si io o X Xa av vi ie er r d de e M Me en nd do on nç ça a J Jú ún ni io or r, , D De en ni is se e S Sa ar re et tt ta a S Sc ch hw wa ar rt tz z, , E Ed du ua ar rd do o H Ha ar rr ry y B Bi ir rg ge el l J Jú ún ni io or r, , E En nr ri ic co o L Li ip pp pi i O Or rt to ol la an ni i, , F Fe er rn na an nd do o J Jo os sé é B Be en ne es si i, , L Li il li ia am m G Gr re eg go or ry y, , M Má ár rc ci ia a M Me er ry y K Ko og gi ik ka a, , M Ma ar ri ia a C Cl lá áu ud di ia a A Ar ra ar ri ip pe e S Su uc cu up pi ir ra a, , M Ma ar ri ia a H He el le en na a A Ak ka ao o L La ar rs ss so on n, , M Mi it ti ik ka a K Ku ur ri ib ba ay ya as sh hi i H Ha ag gi iw wa ar ra a, , R Ra aq qu ue el l Y Yv vo on nn ne e A Ar ra an nt te es s B Ba ac cc ca ar ri in n, , S Si il lv vi ia a R Re eg gi in na a R Ri ic cc ci ...

show abstract

Control of bovine leukaemia virus transmission by selective culling of infected cattle on the basis of viral antigen expression in lymphocyte cultures

Cited by 16 publications

References 12 publications

Controlling bovine leukemia virus in dairy herds by identifying and removing cows with the highest proviral load and lymphocyte counts

Controlling bovine leukemia virus in dairy herds by identifying and removing cows with the highest proviral load and lymphocyte counts

Enzootic bovine leukosis

Influência do vírus da lecucose bovina na resposta imunitária de animais naturalmente infectados

Contact Info

Product

Resources

About