Control of germinal vesicle breakdown in bovine x murine hybrid oocytes

Fulka, J.; Leibfried-Rutledge, ML; First, N.L.

doi:10.1051/rnd:19930501

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…Sendai-virus-mediated fusion of GV-stage oocyte was used to investigate the interaction between the germinal vesicle and cytoplasm 27 28 29 . After cell fusion, it was found that the MPF (maturation-promoting factor) function was not species-specific.…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model

Wang

Zhang

Schatten

et al. 2016

Sci Rep

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Spindle sizes are different in diverse species and cell types. In frogs, the meiotic spindle size is positively correlated with the egg cell volume. Across species, relatively small mouse oocytes (70–80 μm) have a relatively large spindle while larger pig oocytes (about 120 μm) have a considerably smaller spindle. In this study we investigated whether species-specific oocyte spindle size was determined by cytoplasmic or nuclear factors. By exchanging the germinal vesicle between mouse and pig oocytes, we obtained two kinds of reconstructed oocytes: one with mouse ooplasm and pig GV (mCy-pGV oocyte), and the other with pig ooplasm and mouse GV (pCy-mGV oocyte). We show that the MII spindle size of the mCy-pGV oocyte is similar to that of the mouse meiotic spindle and significantly larger than that of the pig meiotic spindle. The timing of oocyte maturation also followed that of the species from which the oocyte cytoplasm arose, although some impact of the origin of the GV was observed. These data suggest that spindle size and the timing of meiotic progression are governed by cytoplasmic components rather than cytoplasmic volume and GV materials.

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Section: Discussionmentioning

confidence: 99%

Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model

Wang

Zhang

Schatten

et al. 2016

Sci Rep

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“…Follicular components also play an important role in the maintenance of the GV stage (Sirard et al, 1992). On the other hand, the cyclic nucleotides, which are known as potent inhibitors of GVBD in rodents (Tornell et al, 1991), do not block GVBD in bovine oocytes (Fulka et al, 1993).…”

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confidence: 99%

Cytoplasmic argyrophilic protein(s) in cumulus-oocyte complexes: a preliminary study

Antalíková

Fulka²,

Horská³

et al. 1994

Reprod. Nutr. Dev.

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Summary ― The presence of acidic argyrophilic protein(s) in cumulus cells and oocytes was examined in bovine, ovine, porcine and murine cumulus-oocyte complexes (COCs) using a specific silverstaining method. The COCs were isolated from small (1 mm) or large (3-5 mm) antral follicles; they were fixed immediately after slaughter (first group) or after transfer to the laboratory (ca 60 min, second group) and then silver stained according to Likovsky'and Smetana

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Effects of ethanol and dimethylsulphoxide on nuclear and cytoplasmic maturation of bovine cumulus-oocyte complexes

Avery¹,

Greve²

2000

Mol. Reprod. Dev.

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Control of germinal vesicle breakdown in bovine x murine hybrid oocytes

Cited by 6 publications

References 11 publications

Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model

Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model

Cytoplasmic argyrophilic protein(s) in cumulus-oocyte complexes: a preliminary study

Effects of ethanol and dimethylsulphoxide on nuclear and cytoplasmic maturation of bovine cumulus-oocyte complexes

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