Plant factories that ensure the annual production of vegetable crops have sparked much attention. In the present study, thirty types of common vegetable crops from 25 species and eight families, were grown in a multi-layer hydroponic system in a closed-type plant factory to evaluate the adaptive performance. A total of 20 vegetable crops, belonging to 14 species and 4 families, unexpectedly exhibited different degrees of leaf margin necrosis in lower leaves firstly, then the upper leaves gradually. We defined this new physiological disorder as “leaf burn”. It occurred more commonly and severely in cruciferous leafy vegetables. Two different light intensities (150 and 105 µmol m−2 s−1 photosynthetic photon flux density (PPFD)), three photoperiod conditions (12, 10 and 8 h d−1) and two canopy relative air humidity (RH) (70% and 90% RH) were set to evaluate the suppression effects on leaf burn occurrence in two commercial flowering Chinese cabbage cultivars (‘Sijiu’ and ‘Chixin’), the special cruciferous vegetable in South China. We discovered that changing light conditions did not fully suppress leaf burn occurrence in the cultivar ‘Sijiu’, though lower light intensity and shorter photoperiod partly did. Interestingly, the occurrence of leaf burn was completely restrained by an increased canopy RH from 70% to 90%. Specifically, the low RH-treated seedlings occurred varying degree of leaf burn symptoms, along with rapidly decreased water potential in leaves, while the high RH treatment significantly lessened the drop in leaf water potential, together with increased photosynthetic pigment contents, net photosynthetic rate, stomatal conductance and transpiration rate, decreased leaf stomatal aperture and density, and thus reduced the incidence of leaf burn in ‘Sijiu’ and ‘Chixin’, from 28.89% and 18.52% to zero, respectively. Taken together, high canopy RH may favor maintaining leaf water potential and improving photosynthesis performance, jointly regulating leaf burn incidence and plant growth.