Control of growth of mammalian cells in cell culture

Holley, Robert W.

doi:10.1038/258487a0

Cited by 527 publications

(137 citation statements)

References 67 publications

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“…Minowada (1978)). The cultured cell lines studied are shown assigned to their apparent stage of differentiation on the basis of marker profiles (Minowada, 1978 (Holley, 1975). Results reported here are reproducible; however, we also found spectral charges attributable to cell density and the time of medium change before inserting samples into the NMR probe.…”

supporting

confidence: 66%

High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Mountford¹,

Grossman²,

Gatenby³

et al. 1980

Br J Cancer

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supporting

confidence: 66%

High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Mountford¹,

Grossman²,

Gatenby³

et al. 1980

Br J Cancer

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“…Nevertheless, there is no question that increased nutrient uptake -by whatever mechanism -gives tumor cells a selective advantage for growth (21). The fact that normal cells in suspension do not grow and their uptake rate is very low, and the fact tumor cells have increased uptake capacity even in the rounded state and do grow may have implications for the avascular phase of tumor survival and metastasis in vivo.…”

Section: Discussionmentioning

confidence: 99%

Cell shape and hexose transport in normal and virus‐transformed cells in culture

1977

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The rate of hexose transport was compared in normal and virus‐transformed cells on a monolayer and in suspension. It was shown that: (1) Both trypsin‐removed cells and those suspended for an additional day in methyl cellulose had decreased rates of transport and lower available water space when compared with cells on a monolayer. Thus, cell shape affects the overall rate of hexose transport, especially at higher sugar concentrations. (2) Even in suspension, the initial transport rates remained higher in transformed cells with reference to normal cells. Scanning electron micrographs of normal and transformed chick cells revealed morphological differences only in the flat state. This indicates that the increased rate of hexose transport after transformation is not due to a difference in the shape of these cells on a monolayer.

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“…Upper layers consisted of 1 ml volumes of a mixture of minimal essential medium (GIBCO, Grand Island, NY) and 15% FBS, in 0.3% agar. Conditioned medium was obtained from the supernatants of three breast tumour cell lines (Hug et al, 1984 (Lippman & Bolan, 1975;Holley, 1975;Gross et al, 1984). Nine tumours were also assayed for the responsiveness to 17-beta-oestradiol alone.…”

Section: Patientsmentioning

confidence: 99%

Predictors of hormone response for patients with ER-unknown breast tumours

Hug

Bentzen²

1989

Br J Cancer

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Summary Characteristics of cells that are associated with the hormonal dependence of tumours are described, and it is shown that clonogenicity and hormone-induced proliferative response of breast tumours are as good markers of hormonal dependence as is oestrogen receptor. Thus tumours that formed less than 150 colonies per 500,000 cells seeded and that increased their proliferative activity 1.8-fold or more in response to hormones were the tumours that were likely to respond to endocrine treatments, whereas all other tumours were likely to be refractory to endocrine treatments. These two criteria (clonogenicity and proliferative response to growth hormones) correctly identified the response to subsequent endocrine treatments in 15 out of 17 patients with oestrogen receptor-unknown tumours. It is proposed that they may constitute a substitute for the oestrogen receptor status in patients with non-biopsiable tumours, and an additional discriminant where the oestrogen receptor assay is available.

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Control of growth of mammalian cells in cell culture

Cited by 527 publications

References 67 publications

High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

High-resolution proton nuclear magnetic resonance: application to the study of leukaemic lymphocytes

Cell shape and hexose transport in normal and virus‐transformed cells in culture

Predictors of hormone response for patients with ER-unknown breast tumours

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