Control ofVibrioparahaemolyticus(AHPND strain) and improvement of water quality using nanobubble technology

Abstract

Nanobubble technology is used in wastewater treatment, but its disinfectant properties in aquaculture have not been clearly demonstrated. This study investigated the ability of nanobubbles to reduce Vibrio parahaemolyticus (AHPND strain) and to improve water quality. Two laboratory experiments were conducted over a one‐week period, that is (a) assessing the effects of air and oxygen nanobubbles for 60 minutes per day and (b) comparing effects of ozone nanobubble treatments for 2, 4 and 6 minutes per day. Experiments were done in triplicate 100 L tanks with 15‰ saline water, inoculated with an initial bacterial concentration of 106 CFU/ml. At the end of experiment 1, the bacterial concentration of the air and oxygen nanobubble groups was counted for 69% and 46% of the control group respectively. At the end of experiment 2, the bacterial concentration of the 2‐, 4‐ and 6‐minute ozone nanobubble groups were counted for 23%, 2.2% and 0% of the control group respectively. Oxygen and ozone nanobubbles significantly increased oxygen reduction potential and oxygen values. Results indicate that under effective dosages nanobubbles can be used in the production farms to control V.parahaemolyticus and increase oxygen levels.

Molecular Hydrogen Increases Quantitative and Qualitative Traits of Rice Grain in Field Trials

“…Importantly, the possible negative influence achieved by other magnesium metabolites and/or possible pH alteration in the metallic magnesium-produced HRW could not be easily ruled out. A similar disadvantage was recently reported when magnesium hydride (MgH 2 ) was used as a preservative for prolonging the vase life of cut flowers [22].…”

“…Hydrogen-rich water (HRW) or saline is a typical form of molecular hydrogen used in plants and medicine, due to its feasibility and safety in laboratory [14]. While the low solubility and short residence time of H 2 limit its wide application in practice, especially in paddy field, previous results have revealed that nano-bubble technology could be applied in aquaculture [22] and environment [23], since this approach could increase the content of targeted gas in water, prolong the time of the gas remaining in liquid, and improve the utilization efficiency of targeted gas. A recent study further discovered that the hydrogen nanobubble water (HNW) could alleviate copper toxicity in Daphnia magna since it could increase the solubility and the residence time of H 2 in water, therefore efficiently enhancing antioxidant capacity [24].…”

CRISPR/Cas12a Based Rapid Molecular Detection of Acute Hepatopancreatic Necrosis Disease in Shrimp

“…While improvement of water quality and oxygen levels can reduce the concentration of AHPND-causing Vibrio ( 39 ), and farmers have used broad-spectrum antibiotics to inhibit gram-negative bacteria and parasites ( 40 ), these measures cannot prevent outbreaks of pathogenic bacteria. Furthermore, inappropriate use of broad-spectrum antibiotics may result in multidrug-resistant bacteria that exacerbate the risk of outbreaks.…”

Effective Purification of Eutrophic Wastewater from the Beverage Industry by Microbubbles

“…Joni et al [17] also reported that the application of MiBs in an aquaculture system increased the DO from 4.5 mg L −1 to 7.9 mg L −1 . In addition to their use in aquaculture facilities, MiBs have been used to improve the water quality of wastewater contaminated with various hazardous materials, such as azo dye [18], methylene blue [19], phenol [6,20], palm oil [8], diesel oil [21], trichloroethylene [22], surfactants [3], pesticides [23], and pathogenic bacteria [24]. MiBs have also been used to remove microalgal cells [25] and planktonic crustaceans [26].…”