Control of lipolysis by natriuretic peptides and cyclic GMP

Lafontan, Max; Moro, Cédric; Berlan, Michel; Crampes, F.; Sengenès, Coralie; Galitzky, Jean

doi:10.1016/j.tem.2007.11.006

Cited by 213 publications

(169 citation statements)

References 68 publications

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“…As mentioned in the introduction to this paper, it has been reported that in human fat cells, lipolysis can be stimulated by atrial natriuretic peptide (ANP) through a cyclic GMP/PKG signalling pathway independent of cyclic AMP production and PKA activity [2]. In the following experiments, we tested whether biguanides and thiazolidinediones can inhibit lipolysis induced by ANP.…”

Section: Resultsmentioning

confidence: 97%

“…Conversely, insulin is the most potent anti-lipolytic hormone, acting through the activation of phosphodiesterase 3B, which induces the degradation of cyclic AMP. In humans it has been reported that natriuretic peptides, particularly atrial natriuretic peptide (ANP), produced by the heart during exercise, could also stimulate lipolysis in adipocytes through a cyclic GMP-dependent protein kinase (PKG) signalling pathway independently of cyclic AMP pathways [2].…”

Section: Introductionmentioning

confidence: 99%

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Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

et al. 2009

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Aims/hypothesis In rodent adipocytes, activated AMPactivated protein kinase reduces the lipolytic rate. As the hypoglycaemic drugs metformin and thiazolidinediones activate this enzyme in rodents, we tested the hypothesis that in addition to their known actions they could have an anti-lipolytic effect in human adipocytes. Methods Adipose tissue was obtained from individuals undergoing plastic surgery. Adipocytes were isolated and incubated with lipolytic agents (isoprenaline, atrial natriuretic peptide) and biguanides or thiazolidinediones. Lipolysis was quantified by the glycerol released in the medium. AMPactivated protein kinase activity and phosphorylation state were determined using standard procedures. Results In human adipocytes, isoprenaline and atrial natriuretic peptide stimulated the lipolytic rate three-to fourfold. Biguanides and thiazolidinediones activated AMPactivated protein kinase and inhibited lipolysis by 30-40%, at least in part by inhibiting hormone-sensitive lipase translocation to the lipid droplet. Inhibition of AMPactivated protein kinase by compound C precluded this inhibitory effect on lipolysis. Stimulation of lipolysis also induced an activation of AMP-activated protein kinase concomitant with a drop in ATP concentration. Conclusions/interpretation We show for the first time in human adipocytes that biguanides and thiazolidinediones activate AMP-activated protein kinase, thus counteracting lipolysis induced by lipolytic agents. In addition, β-agonistor ANP-stimulated lipolysis increases AMP-activated protein kinase activity. This is because of an increase in the AMP/ATP ratio, linked to activation of some of the released fatty acids into acyl-CoA. AMP-activated protein kinase activation could represent a physiological means of avoiding a deleterious drain of energy during lipolysis but could be used to restrain pharmacological release of fatty acids.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

et al. 2009

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“…BNP/NP has been shown to activate lipolysis and increase FFA release [39,41]. Accordingly, a decrease in BNP/NP production in obesity may serve to reduce the excessive release of FFA and thereby attenuate the deleterious effects of toxic FA derivatives [12,42,43].…”

Section: Resultsmentioning

confidence: 99%

Cardiac production of B-type natriuretic peptide is inversely related to the plasma level of free fatty acids in obese individuals - possible involvement of the insulin resistance -

Mizuno¹,

Harada²,

Katoh

et al. 2013

Endocr J

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“…ANP and BNP, but not CNP, have been reported to induce potent lipolytic effects in human adipocytes similar to those exerted by the b-adrenoceptor agonist isoproterenol (360,424) . Moreover, ANP inhibits human visceral adipocyte growth in culture at physiological concentrations (425) .…”

Section: Atrial Natriuretic Peptidesmentioning

confidence: 96%

Peripheral signalling involved in energy homeostasis control

2012

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The alarming prevalence of obesity has led to a better understanding of the molecular mechanisms controlling energy homeostasis. Regulation of energy intake and expenditure is more complex than previously thought, being influenced by signals from many peripheral tissues. In this sense, a wide variety of peripheral signals derived from different organs contributes to the regulation of body weight and energy expenditure. Besides the well-known role of insulin and adipokines, such as leptin and adiponectin, in the regulation of energy homeostasis, signals from other tissues not previously thought to play a role in body weight regulation have emerged in recent years. The role of fibroblast growth factor 21 (FGF21), insulin-like growth factor 1 (IGF-I), and sex hormone-binding globulin (SHBG) produced by the liver in the regulation of body weight and insulin sensitivity has been recently described. Moreover, molecules expressed by skeletal muscle such as myostatin have also been involved in adipose tissue regulation. Better known is the involvement of ghrelin, cholecystokinin, glucagon-like peptide 1 (GLP-1) and PYY 3 -36 , produced by the gut, in energy homeostasis. Even the kidney, through the production of renin, appears to regulate body weight, with mice lacking this hormone exhibiting resistance to diet-induced obesity. In addition, the skeleton has recently emerged as an endocrine organ, with effects on body weight control and glucose homeostasis through the actions of bone-derived factors such as osteocalcin and osteopontin. The comprehension of these signals will help in a better understanding of the aetiopathology of obesity, contributing to the potential development of new therapeutic targets aimed at tackling excess body fat accumulation.

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Control of lipolysis by natriuretic peptides and cyclic GMP

Cited by 213 publications

References 68 publications

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

Biguanides and thiazolidinediones inhibit stimulated lipolysis in human adipocytes through activation of AMP-activated protein kinase

Cardiac production of B-type natriuretic peptide is inversely related to the plasma level of free fatty acids in obese individuals - possible involvement of the insulin resistance -

Peripheral signalling involved in energy homeostasis control

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