Control of microbial biofilm formation as an approach for biomaterials synthesis

Chaves, Silvina; Longo, Marianella; López, Azucena Gómez; Loto, Flavia del Valle; Mechetti, Magdalena; Romero, Cintia Mariana

doi:10.1016/j.colsurfb.2020.111201

Cited by 8 publications

(8 citation statements)

References 41 publications

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“…The formation of biofilm by microorganisms is an essential feature from which it is possible to analyze their binding to the surface of roots for different functions in their community (Chaves et al, 2020). The selected strains were grown on LB liquid culture in order to know the effect of various salt concentrations on biofilm formation.…”

Section: Biofilm Formation Assay Under Salt Stressmentioning

confidence: 99%

Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress

Ali

Ayaz

Mu³

et al. 2022

Front. Plant Sci.

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Soil salinity is a major environmental stress that has been negatively affecting the growth and productivity of rice. However, various salt-resistant plant growth-promoting rhizobacteria (PGPR) have been known to promote plant growth and alleviate the damaging effects of salt stress via mitigating physio-biochemical and molecular characteristics. This study was conducted to examine the salt stress potential of Bacillus strains identified from harsh environments of the Qinghai-Tibetan plateau region of China. The Bacillus strains NMTD17, GBSW22, and FZB42 were screened for their response under different salt stress conditions (1, 4, 7, 9, 11, 13, and 16%). The screening analysis revealed strains NMTD17, GBSW22, and FZB42 to be high-salt tolerant, moderate-salt tolerant, and salt-sensitive, respectively. The NMTD17 strain produced a strong biofilm, followed by GBSW22 and FZB42. The expression of salt stress-related genes in selected strains was also analyzed through qPCR in various salt concentrations. Further, the Bacillus strains were used in pot experiments to study their growth-promoting ability and antioxidant activities at various concentrations (0, 100, 150, and 200 mmol). The analysis of growth-promoting traits in rice exhibited that NMTD17 had a highly significant effect and GSBW22 had a moderately significant effect in comparison with FZB42. The highly resistant strain NMTD17 that stably promoted rice plant growth was further examined for its function in the composition of rhizobacterial communities. The inoculation of NMTD17 increased the relative abundance and richness of rhizobacterial species. These outcomes propose that NMTD17 possesses the potential of PGPR traits, antioxidants enzyme activities, and reshaping the rhizobacterial community that together mitigate the harmful effects of salinity in rice plants.

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Section: Biofilm Formation Assay Under Salt Stressmentioning

confidence: 99%

Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress

Ali

Ayaz

Mu³

et al. 2022

Front. Plant Sci.

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“… 1 Bacteria attach to biomaterial surfaces and form biofilm. 2 Pathogenic biofilms are involved in ≥65% of nosocomial infections. 3 A mature biofilm is more difficult to eliminate than planktonic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Plasma SiOx:H Nanocoatings to Enhance the Antibacterial and Anti-Inflammatory Properties of Biomaterials

Ye¹,

Yu²,

Dong³

et al. 2022

IJN

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Purpose To evaluate the antibacterial and anti-inflammatory properties of SiOx:H nanocoatings using a plasma-deposition technique. Materials and Methods Four groups of SiOx:H nanocoatings were prepared by plasma nanocoating technique using different deposition gases and durations, specifically trimethylsilane (TMS) for groups A1 and A2 and a mixture of TMS and oxygen for groups B1 and B2. Changes in surface chemistry and physical properties were measured. Staphylococcus aureus and Streptococcus mutans were cultured on plasma SiOx:H nanocoatings to evaluate antibacterial and antibiofilm formation activities. Human gingival fibroblasts (HGFs) and HaCaT human keratinocytes were cultured and stimulated with tumor necrosis factor-α (TNF-α). Cell viability was measured using a Cell Counting Kit-8 (CCK-8) assay. Quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to evaluate anti-inflammatory properties, including the mRNA and protein levels of inflammatory mediators and proinflammatory cytokines. Results The carbon content was dominant in group A nanocoatings and the oxygen and silicon elements were dominant in group B nanocoatings. Groups A2 and B2 were approximately threefold thicker than groups A1 and B1. The plasma SiO x :H nanocoatings decreased bacterial growth and biofilm formation by 30–70% ( p < 0.05). Scanning electron microscopy (SEM) revealed damaged biofilm structures. Moreover, the antibacterial properties of group B were greater than group A, and the antibacterial properties of groups A2 and B2 were more effective than A1 and B1, respectively. CCK-8 assays revealed the plasma SiOx:H nanocoatings had good biocompatibility. Furthermore, under TNF-α-induced inflammation, the mRNA and protein levels of interleukin-6, interleukin-8, cyclooxygenase-2, and monocyte chemoattractant protein-1 were downregulated in the plasma SiOx:H nanocoating groups ( p < 0.05). Conclusion Plasma SiOx:H nanocoatings exerted antibacterial and anti-inflammatory effects with excellent biocompatibility. Therefore, the plasma SiOx:H nanocoating technique has potential for implant materials and other medical devices.

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“…The nutrient concentration and more generally the composition of the substrate influence the composition of the biofilm matrix and subsequently biofilm properties. 4,9,11 In B. subtilis biofilms for example, the composition of the growth medium was shown to actively contribute 16 to the production, the distribution and the characteristics of the amyloid fibers found in the extracellular matrix. 4 V. fischeri biofilms also acquire different morphologies and resistance to mechanical disruption depending on the nutrients available to the bacteria.…”

Section: 35mentioning

confidence: 99%

“…2 The availability of nutrients is also proposed to play a major role in the growth, phenotype, mechanical properties and matrix composition. [2][3][4] For instance, nutrient limitations in the substrate might lead to spatial gradients of activities and molecules influencing the functioning of biofilms. 5 Variation of nutrients availability and their effect on different biofilm aspects have been studied for different bacteria such as Bacillus sp., 4,6 P. fluorescence 7,8 and V. fischeri 9 .…”

Section: Introductionmentioning

confidence: 99%

Nutrient availability influencesE. colibiofilm properties and the structure of purified curli amyloid fibers

Siri,

Vázquéz-Dávila,

Bidan

2023

Preprint

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Bacterial biofilms are highly adaptable and resilient to challenges. Nutrient availability can induce changes in biofilm growth, biomass, morphology, architecture and mechanical properties. Bacterial extracellular matrix plays a major role in achieving biofilm stability under different environmental conditions. Curli amyloid fibers are determining for the architecture and stiffness ofE. colibiofilms, but how this major matrix component adapts to different environmental cues remains unclear.Here, we investigated the effect of nutrient availability on both i) biofilm materials properties and ii) the structure and properties of curli amyloid fibers extracted from the biofilms. For this, we culturedE. coliW3110, which main matrix component is curli fibers. We quantified the size, mass and water content of the resulting biofilms and estimated their mechanical properties by microindentation. The curli amyloid fibers were then purified from the biofilms and their molecular structure and properties were studied by spectroscopic techniques. Our results show that the availability of nutrients in the substrate influences the yield of curli fibers, their structural composition and chemical stability, and suggest that these molecular features contribute to the stiffness of the biofilms. Biofilms grown on substrates with high nutrient concentration are softer, contain less curli fibers, and these fibers exhibit low β-sheet content and chemical stability.Our multiscale study sheds new light on the relationship between the molecular structure of bacterial matrix and the macroscopic properties of biofilms. This knowledge will benefit the development of both anti-biofilm strategies and biofilm-based materials.Graphical abstract

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Control of microbial biofilm formation as an approach for biomaterials synthesis

Cited by 8 publications

References 41 publications

Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress

Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress

Plasma SiOx:H Nanocoatings to Enhance the Antibacterial and Anti-Inflammatory Properties of Biomaterials

Nutrient availability influencesE. colibiofilm properties and the structure of purified curli amyloid fibers

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