Control of Plant Diseases by Chitinase Expressed from Cloned DNA inEscherichia coli

PCR cloning and heterologous expression of chitinase gene of endophytic Streptomyces aureofaciens CMUAc130

“…The results presented in this work confirm the role of chitinases in the antifungal activity of various microbial antagonists (Lorito et al, 1993;Ordentlich et al, 1988;Shapira et al, 1989;Tsujibo et al, 2000). The S. aureofaciens CMUAc130 chitinase gene could therefore be used to transform other bacteria or plants and provide them with the ability to control fungal phytopathogens.…”

Current Advances in the Fusarium Wilt Disease Management in Banana with Emphasis on Biological Control

“…These compounds have direct effect on the growth of the pathogens. Biocontrol bacteria producing chitinase (Shapira et al, 1989;Dunne et al, 1996;Ross et al, 2000), protease (Dunlap et al, 1997;Dunne et al, 1998), cellulase (Chatterjee et al, 1995) or glucanases (RuizDuenas & Martinez, 1996;Jijakli & Lepoivre, 1998) were shown to suppress plant diseases as these enzymes are involved in the breakdown of fungal cell walls by degrading cell wall constituents such as glucans and chitins, resulting in the destruction of pathogen structures or propagules. The bacteria also play a major role in growth promotion by producing phytohormones such as auxins, gibberellins, cytokinins and ethylene (García de Salamone et al, 2001;Remans et al, 2008).…”

Chitinases from the Plant Disease Biocontrol Agent, Stenotrophomonas maltophilia C3

“…Application of chitin to soils or foliage increased numbers of chitinolytic bacteria along with providing pathogen control (10,17). In addition, bacterial chitinase genes, when introduced into heterologous strains, provided antifungal activity (26,29), or when introduced into plants, conferred increased disease resistance (15). However, few studies on bacterial agents have provided direct evidence, through analysis of mutants lacking chitinase activity or through assessment of extracted chitinase for biological activity, that chitinase had a role (4,14,26).…”

“…In addition, bacterial chitinase genes, when introduced into heterologous strains, provided antifungal activity (26,29), or when introduced into plants, conferred increased disease resistance (15). However, few studies on bacterial agents have provided direct evidence, through analysis of mutants lacking chitinase activity or through assessment of extracted chitinase for biological activity, that chitinase had a role (4,14,26). We recently reported Stenotrophomonas maltophilia strain C3 to be a biocontrol agent, active in part through chitinase activity (38).…”