PCR cloning and heterologous expression of chitinase gene of endophytic Streptomyces aureofaciens CMUAc130

Taechowisan, Thongchai; Peberdy, John F.; Lumyong, Saisamorn

doi:10.2323/jgam.50.177

Cited by 7 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The confirmation of this protective effect in soil is now in process. As reported by other studies, this protective effect might be increased by the ability of the selected Actinomycete strains to excrete chitinases (Taechowisan et al 2004), IAA (Martinez-Noel et al 2001), siderophores (Matthijs et al 2007;Hamdali et al 2008) and/or to other anti-fungal substances (Xiao et al 2002;Errakhi et al 2007). Furthermore, the strains having the most important stimulatory effect on plant growth and protective effect against the phytopathogenic fungi were also those able to colonize the external surface of the roots as well as to develop inside the roots.…”

Section: Discussionmentioning

confidence: 79%

Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities

Hamdali

Hafidi

Virolle

et al. 2008

World J Microbiol Biotechnol

163

View full text Add to dashboard Cite

In order to select Actinobacteria that could improve plant growth and thus agricultural yield, we assessed different plant growth promoting (PGP) abilities of eight rock phosphate (RP)-solubilizing Actinomycete isolates originating from Moroccan phosphate mines. Six of these strains were able to grow on root exudates of the wheat plant as sole nutrient sources and were efficiently releasing soluble phosphate from RP. These strains also inhibited the growth of potentially phytopathogenic fungi, bacteria (Gram +/-) or yeasts. Five of these strains produced indoleacetic acid and four showed endophytic properties. When these strains were grown, in the presence of the wheat plant, in a synthetic minimum medium (SMM) containing insoluble RP as unique phosphate source or in soil experiment, the most active RP-solubilizing strains had the highest stimulatory effect on the production of plant biomass. The most efficient strain Streptomyces griseus-related strain (BH 7 ), stimulated aerial growth of the plant more than 70% in test tubes and more than 30% in RP soil compared to the non-inoculated control treatment. This study demonstrated that our selected Actinomycete strains could be used for the development of novel, non-polluting; farming practices by entering in the formulation of novel biofertilizer and biocontrol products constituted by spores and/or mycelium of the ad hoc Actinobacteria in association with pulverized RP.

show abstract

Section: Discussionmentioning

confidence: 79%

Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities

Hamdali

Hafidi

Virolle

et al. 2008

World J Microbiol Biotechnol

163

View full text Add to dashboard Cite

show abstract

“…The confirmation of this protective effect in soil is now in process. As reported by other studies, this protective effect might be increased by the ability of the selected Actinomycete strains to excrete chitinases (Taechowisan et al 2004), IAA (Martinez-Noel et al 2001), siderophores (Matthijs et al 2007;Hamdali et al 2008a;Cao et al 2005) a Sample from the untreated (control) grapevine leaves; b leaves inoculated with actinomycetes (isolate SS38); c leaves inoculated with B. cinerea and exhibiting the symptoms of gray mold disease; The hyphae of the pathogen abundantly colonize the epidermis and the parenchyma. Fungal growth occurs both intracellularly and intercellularly.…”

Section: Discussionmentioning

confidence: 89%

Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold

Loqman

Barka

Clément

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

One hundred and forty-two different actinomycete strains were isolated from rhizosphere soil of Vitis vinifera L. sampled from four Moroccan areas. To evaluate the antifungal effect of the different collected actinomycete isolates, five fungi known to be phytopathogens (Pythium ultimum, Fusarium oxyysporum f. sp. albedinis, Sclerotium rolfsii, Verticillium dahliae and Botrytis cinerea) were used. Results showed that 24 isolates had an in vitro inhibitory effect toward at least 4 of the indicator fungi, but only 9 inhibited all these phytopathogens. These nine isolates were subsequently evaluated individually using in vitro grapevine plantlets for their ability to protect against plant gray mold. We demonstrate here that pre-inoculation of plantlets with these isolates allow them to withstand Botrytis cinerea. Six of these strains were shown to belong to the genus Streptomyces and three to the genus Micromonospora. These findings indicate the potential of developing effective actinomycetes from Moroccan habitats for the biological control of Botrytis cinerea.

show abstract

“…Two out of the eleven selected actinobacterial isolates were shown to be efficiently able to limit growth, at least on Petri dishes, of the phytopathogenic Fusarium sp. Several recent studies demonstrated that this protective effect might be increased by the ability of the selected actinobacterial strains to excrete chitinases (Taechowisan et al 2004), IAA (Martinez-Noel et al 2001), siderophores (Matthijs et al 2007;Hamdali et al 2008) and/or to other anti-fungal substances (Xiao et al 2002;Errakhi et al 2007). This significant feature is interesting since they are able to contribute in the biological control of soil-born diseases and they can also protect plant root surfaces from colonization by pathogenic microbes through direct competitive effects and production of antimicrobial agents.…”

Section: Discussionmentioning

confidence: 97%

Isolation and characterization of non-Frankia actinobacteria from root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia

et al. 2009

View full text Add to dashboard Cite

Actinobacterial isolates randomly obtained on nitrogen-free BAP medium from surface sterilized root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia sampled from fields were reported. They were assigned on the basis of partial 16S rRNA sequences to Micromonospora, Nocardia and Streptomyces genera. The isolates have been screened for hydrolytic activities, indole acetic acid (IAA) and siderophores production, phosphate solubilization and antagonistic activities. Results suggest putative traits as plant growth promoting bacteria proprieties of the isolates that occur in unique association in root nodules of the three analysed actinorhizal host species.

show abstract

PCR cloning and heterologous expression of chitinase gene of endophytic Streptomyces aureofaciens CMUAc130

Cited by 7 publications

References 21 publications

Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities

Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities

Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold

Isolation and characterization of non-Frankia actinobacteria from root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia

Contact Info

Product

Resources

About