Control of Precocious Gladiolus Corm and Cormel Formation in Tissue Culture

Steinitz, Benjamin; Lilien-Kipnis, H.

doi:10.1016/s0176-1617(89)80110-5

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…'Kinneret' were used as initial explants. Sterilization of the plant material and preparation of culture media were as described by Steinitz & Lilien-Kipnis (1989). A stock of plantlets for experiments was prepared on a proliferation medium which consisted of Murashige & Skoog (1962) major and minor salts, 100mg 1 -I myo-inositol, 0.5 mg 1-1 pyridoxine HC1, 0.1 mg 1-1 thiamine HCI, supplemented with 3% sucrose, 0.3/xM BA and 0.1/zM NAA.…”

Section: Methodsmentioning

confidence: 99%

“…The dose response relations for the influence of BA on gladiolus corm formation were investigated by us before but with a different approach (Steinitz & Lilien-Kipnis 1989): shoots were grown on a proliferation medium including 0.3/xM BA and subsequently transferred to a liquid medium with different concentrations of BA. Although BA was not added to the liquid gladiolus corm growth.…”

Section: The Cooperation Between Paclobutrazol and Bamentioning

confidence: 99%

“…Shoots derived from these plantlets were then incubated for one month in a liquid medium including 60 g 1 1 sucrose and 10 mg I-l paclobutrazol, medium of the control plants (0 BA in Fig. 5, Steinitz & Lilien-Kipnis 1989) these plants received a 0.3/~M BA treatment during the stage of shoot development on the proliferation medium, which is equivalent to a 0.3/xM BA treatment in the dose response curve described in Fig. 7 of the present work.…”

Section: The Cooperation Between Paclobutrazol and Bamentioning

confidence: 99%

“…Recently Steinitz & Lilien-Kipnis (1989) and Ziv (1989) found that in vitro gladiolus corm and cormel formation also can be promoted by treating bud and shoot explants with paclobutrazol. Since precocious paclobutrazol-mediated corm formation could be prevented by addition of GA 3 to the medium, it was suggested that paclobutrazol promotes corm growth by inhibition of gibberellin biosynthesis (Steinitz & Lilien-Kipnis 1989).…”

Section: Introductionmentioning

confidence: 99%

“…2992-E, 1990 series. ed corm formation at the base of the shoot (Steinitz & Lilien-Kipnis 1989). This inconsistency can presumably be resolved by a further search for the appropriate developmental stage of application of the correct cytokinin concentration.…”

Section: Introductionmentioning

confidence: 99%

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Precocious gladiolus corm formation in liquid shake cultures

Steinitz

Cohen

Goldberg

et al. 1991

Plant Cell Tiss Organ Cult

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Conditions were defined for precocious differentiation and improved growth of corms at the base of gladiolus shoots. Shoots were derived from explants cultured on agar solidified media, and corm regeneration was obtained in subsequent liquid shake cultures. Benzyladenine (BA), at 10 .7 M, was found to have a stimulating effect mainly when provided to the shoots prior to manifestation of corm growth. Paclobutrazol and sucrose promoted corm formation when supplemented to the liquid media. Paclobutrazol, at 10mg 1 -l, shifted assimilate allocation towards the growing corm. A differential promotion of corm development by sucrose was not observed, and the concentration of sucrose at which the sugar demand for maximal shoot and corm growth is satisfied (60 g 1-1) was unaltered by the presence of paclobutrazol. The rate of corm growth on shoots cultured in a liquid medium supplemented with paclobutrazol and a saturating sucrose concentration, was a function of the length of the shoot's leaf blades, and was similar in light and in dark.

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Section: Methodsmentioning

confidence: 99%

Section: The Cooperation Between Paclobutrazol and Bamentioning

confidence: 99%

Section: The Cooperation Between Paclobutrazol and Bamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations