Objective: To characterize the relationship between serum carotenoids, retinol and anaemia among pre-school children. Design: A cross-sectional study was conducted in two groups: anaemic and non-anaemic. Serum levels of retinol, a-carotene, b-carotene, b-cryptoxanthin, lycopene, lutein and zeaxanthin were measured in the study subjects. Setting: Six rural communes of Dinh Hoa, a rural and mountainous district in Thai Nguyen Province, in the northern mountainous region of Vietnam. Subjects: A total of 682 pre-school children, aged 12-72 months, were recruited. Results: Geometric mean serum concentrations of carotenoids (mmol/l) were 0?056 for a-carotene, 0?161 for b-carotene, 0?145 for b-cryptoxanthin, 0?078 for lycopene, 0?388 for lutein and 0?075 for zeaxanthin. The mean levels of Hb and serum retinol were 108?8 g/l and 1?02 mmol/l, respectively. The prevalence of anaemia and vitamin A deficiency was 53?7 % and 7?8 %, respectively. After adjusting for sex and stunting, serum retinol concentrations (mmol/l; OR 5 2?06, 95 % CI 1?10, 3?86, P 5 0?024) and total provitamin A carotenoids (mmol/l; OR 5 1?52, 95 % CI 1?01, 2?28, P 5 0?046) were independently associated with anaemia, but non-provitamin A carotenoids (mmol/l; OR 5 0?93, 95 % CI 0?63, 1?37, P 5 0?710) were not associated with anaemia. Conclusions: Among pre-school children in the northern mountainous region of Vietnam, the prevalences of vitamin A deficiency and anaemia are high, and serum retinol and provitamin A carotenoids are independently associated with anaemia. Further studies are needed to determine if increased consumption of provitamin A carotenoids will reduce anaemia among pre-school children.