Controllable Molecule Transport and Release by a Restorable Surface-tethered DNA nanodevice

Abstract

In this paper, we report a novel surface-tethered DNA nanodevice that may present three states and undergo conformational changes under the operation of pH. Besides, convenient regulation on the electrode surface renders the construction and operation of this DNA nanodevice restorable. To make full use of this DNA nanodevice, ferrocene (Fc) has been further employed for the fabrication of the molecular device. On one hand, the state switches of the DNA nanodevice can be characterized conveniently and reliably by the obtained electrochemical signals from Fc. On the other hand, β-cyclodextrin-ferrocene (β-CD-Fc) host-guest system can be introduced by Fc, which functionalizes this molecular device. Based on different electrochemical behaviors of β-CD under different states, this DNA nanodevice can actualize directional loading, transporting and unloading of β-CD in nanoscale. Therefore, this DNA nanodevice bares promising applications in controllable molecular transport and release, which are of great value to molecular device design.

Mix‐and‐match nanobiosensor design: Logical and spatial programming of biosensors using self‐assembled DNA nanostructures

“…This system detects miRNA using a gold-plated electrode in the picomolar regime, indicating the potential of DNA origami electrochemical sensors for highly sensitive clinical cancer diagnosis applications. Similarly, other DNA nanostructure-based electrochemical sensors have been developed to detect pH, proteins, and small molecules (Lubin & Plaxco, 2010;Morales et al, 2015;Wang et al, 2016).…”

“…The binding of the bioanalyte (left) with the ssDNA‐associated bioreceptor (center) on the surface of the DNA origami is transduced as a measurable change in properties (right) that can be recognized and quantified by a detector. Figures in the right column are adapted from Chai, Xie, & Grotewold, ; Chakraborty, Veetil, Ja rey, & Krishnan, ; Michelotti, Johnson‐Buck, Manzo, & Walter, ; Ranjbar & Hafezi‐Moghadam, ; Wang et al, , respectively…”

“…ProteinsRinker et al, 2008Hariadi et al, 2015Lubin et al, 2010Douglas et al, 2012 IonsKuzuya et al, 2014Kuzyk et al, 2017Wang et al, 2016Kuzyk et al, 2017 Small molecules Walter et al, 2017 Walter et al, 2017 Walter et al, 2017 Morales et al, 2015 Douglas et al, 2012 Note. It should be noted that several of the references repeat within the table to indicate the versatility of a singular architecture to be adapted to multiple analytes or transduction mechanisms.…”

Reconfigurable Nanopolygons Made of DNA Catenanes

“…In this context, catenanes made of DNA − have come into focus as switchable supramolecular devices that are held together by noncovalent, interlocked, mechanical bonds and thus exhibit unique dynamic properties. Considerable research efforts were directed toward the synthesis of artificial nanomachines based on catenanes. , External triggers such as changes in pH, − strand displacing ODNs as fuel/antifuel sequences, ,, and metal ions/ligands , have been used to mechanically reconfigure catenated nanostructures which were then used as reversible switches or motors. The ability of DNA catenanes to precisely control the motion of their interlocking components is an essential prerequisite for the development of highly complex and controllable dynamic DNA nanomachines. − We have previously described the efficient synthesis of dsDNA catenanes containing single-stranded (ss) gaps in each ring, as well as other mechanically interlocking DNA nanostructures. ,− The ss gap in the catenane allows for the interlocking rings to be controlled by hybridization (Cat hyb ) or release (Cat mec ) with a complementary sequence (cs), changing from an immobile to a dynamic state in which the two rings can move freely within the interlocked space.…”