Controlled Arrangement of Neuronal Cells on Surfaces Functionalized with Micropatterned Polymer Brushes

Pardo-Figuerez, María; Martin, Neil R.W.; Player, Darren J.; Roach, Paul; Christie, S.; Capel, Andrew J.; Lewis, Mark P.

doi:10.1021/acsomega.8b01698

Cited by 24 publications

(13 citation statements)

References 56 publications

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“…In this present work, this material was deposited onto both planar glass substrates as well as poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) polymer brush coated glass. PKSPMA polymer brushes have previously been shown to be non-toxic toward the SH-SY5Y neuroblastoma, and due to their hydrophilicity and charged surfaces shown to inhibit cellular adhesion ( Pardo-Figuerez et al, 2018 ). As such when combined with a secondary material that promotes cellular adhesion this polymer is an ideal selection for creating pre-determined regions of neuronal patterning.…”

Section: Introductionmentioning

confidence: 99%

Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

Capel

Smith

Taccola

et al. 2021

Front. Cell Dev. Biol.

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Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process.

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Section: Introductionmentioning

confidence: 99%

Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

Capel

Smith

Taccola

et al. 2021

Front. Cell Dev. Biol.

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“…Mask-based techniques can generate two or more chemistries via immobilized initiators or graft polymers on uncovered areas . According to these techniques, many chemical patterns have been fabricated.…”

Section: Multichemical Signalsmentioning

confidence: 99%

Decoration of Material Surfaces with Complex Physicochemical Signals for Biointerface Applications

Shi

Liu

Zhang

et al. 2020

ACS Biomater. Sci. Eng.

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The surface properties of biomaterials are crucial at controlling biological interactions. Cells or tissues sense different stimuli from surfaces and respond accordingly. A number of studies have reported that fabricating complex stimuli-presenting surfaces is beneficial for mimicking and understanding the in vivo scenario where multi-physicochemical cues are present. Biological responses toward these surfaces could be either negative, such as immune responses, or positive, such as tissue regeneration. An ideal material surface should, therefore, be multifunctional, triggering the desired biological process and suppressing the unwanted side effects. The methods for material surface decoration can be very sophisticated, depending on the applications such as biosensors, medical devices, and/or implants. To date, decorating material surfaces with complex chemistries and topographies is still challenging, and methods are not straightforward. The majority of the methods require multiple steps and combinational approaches that include mask-based techniques, lithography, wet or dry etching, wet chemistry, or vapor-based coatings, among others. Although these methods have been established in the laboratory, easy-to-access and straightforward approaches need to be explored. This Review summarizes the state-of-the-art techniques for generating patterned multichemical and multitopographic signals on material surfaces, and the potential of having these surfaces in biointerface applications.

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“…Indeed, it is possible to control the freeform morphology of neurons using polymer brushes, which allows control at the micro-level (20µm). 143 Physical patterning methods (such as the use of electrospinning polymers, 3D printing, focussed ion beam and other micro-patterning methods) can also be used to generate surfaces on materials with micro- and nano-patterned features, which alters myogenic differentiation. 144 – 151 These methods could be used to chemically and/or physically pattern the necessary ‘morphological template’ for the differentiation of intrafusal fibre subtypes (i.e.…”

Section: Future Directions and Challengesmentioning

confidence: 99%

Generating intrafusal skeletal muscle fibres in vitro: Current state of the art and future challenges

et al. 2020

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Intrafusal fibres are a specialised cell population in skeletal muscle, found within the muscle spindle. These fibres have a mechano-sensory capacity, forming part of the monosynaptic stretch-reflex arc, a key component responsible for proprioceptive function. Impairment of proprioception and associated dysfunction of the muscle spindle is linked with many neuromuscular diseases. Research to-date has largely been undertaken in vivo or using ex vivo preparations. These studies have provided a foundation for our understanding of muscle spindle physiology, however, the cellular and molecular mechanisms which underpin physiological changes are yet to be fully elucidated. Therefrom, the use of in vitro models has been proposed, whereby intrafusal fibres can be generated de novo. Although there has been progress, it is predominantly a developing and evolving area of research. This narrative review presents the current state of art in this area and proposes the direction of future work, with the aim of providing novel pre-clinical and clinical applications.

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Controlled Arrangement of Neuronal Cells on Surfaces Functionalized with Micropatterned Polymer Brushes

Cited by 24 publications

References 56 publications

Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance

Decoration of Material Surfaces with Complex Physicochemical Signals for Biointerface Applications

Generating intrafusal skeletal muscle fibres in vitro: Current state of the art and future challenges

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