Controlled Assays for Phenotyping the Effects of Strigolactone-Like Molecules on Arbuscular Mycorrhiza Development

Torabi, Salar; Varshney, Kartikye; Villaécija-Aguilar, José Antonio; Keymer, Andreas; Gutjahr, Caroline

doi:10.1007/978-1-0716-1429-7_13

Cited by 11 publications

(11 citation statements)

References 38 publications

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“…Seed germination and inoculation with R. irregularis strains was performed according to Torabi et al, (2021) 44 . L. japonicus seeds were manually scarified with sand paper and surface sterilized with a solution containing 0.3% NaClO and 0.1% SDS for 5 minutes and then washed five time with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

Resolving the haplotypes of arbuscular mycorrhizal fungi highlights the role of two nuclear populations in host interactions

Sperschneider

Yildirir

et al. 2023

Preprint

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Arbuscular mycorrhizal fungi (AMF) are prominent root symbionts with a multinucleate cytoplasm that can carry thousands of nuclei deriving from two parental strains and varying in relative abundance in a large syncytium. Here, we set out to improve our understanding of such remarkable genetics by resolving the nuclear genomes of all publicly available AMF heterokaryons using PacBio HiFi and Hi-C sequencing. We find that all AMF heterokaryons carry two sets of homologous chromosomes, where genes associated with plant colonization reside in gene-sparse, repeat-rich compartments. The co-existing nuclear genomes are phylogenetically related but differ significantly in content and epigenetics, resulting in nucleus-specific regulatory programs during mycorrhizal interactions. AMF heterokaryons carry signatures of past genetic exchange indicative of sexual reproduction, followed by clonal haplotype evolution. This work uncovers the contribution and origin of nuclear genomes present in AMF heterokaryons and opens avenues for improvement and environmental application of these strains.

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Section: Methodsmentioning

confidence: 99%

Resolving the haplotypes of arbuscular mycorrhizal fungi highlights the role of two nuclear populations in host interactions

Sperschneider

Yildirir

et al. 2023

Preprint

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“…Therefore, B. distachyon is an ideal model to study monocotyledonous root system formation in grasses as it possesses all root types of domesticated cereals but tends to be less complex and smaller allowing efficient imaging and root growth analysis. Furthermore, B. distachyon is able to functionally associate with arbuscular mycorrhiza, which requires substantial transcriptional reprogramming in root cortex cells and the development of symbiotic interfaces between plant and fungi (Buendia et al, 2019b;Hong et al, 2012;Müller et al, 2020;Torabi et al, 2021). It was shown, for example, that microbial symbiotic signals like Lipo-chitooligosaccharides stimulate lateral root formation in B. distachyon much like in legumes (Buendia et al, 2019a).…”

Section: Development Of Monocotyledonous Root Systems and Root Hair P...mentioning

confidence: 99%

The wild grass Brachypodium distachyon as a developmental model system

Raissig

Woods

2022

Current Topics in Developmental Biology

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“…For AM analysis, the tissue was incubated for 4 h in EtOH (70%) at room temperature, then transferred to KOH (20%) for 2-3 days and washed three times with water. Later, the root segments were treated with HCl (0.1 M) for 1-2 h, and the solution was replaced by PBS containing 1 µg/ml -1 of WGA-Alexa Fluor 488 (Torabi et al ., 2021). The root segments inoculated with the fluorescent bacteria, the transgenic roots harbouring the pAldolase::tYFP-nls and pAldolase::Aldolase1-YFP constructs, and the tissue stained with WGA-Alexa Fluor 488 were mounted onto microscope slides for observation.…”

Section: Methodsmentioning

confidence: 99%

Aromatic amino acid biosynthesis by aLotusAldolase impacts root hair development and symbiotic associations

Montiel

James

García-Soto³

et al. 2022

Preprint

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Legume roots can be symbiotically colonized by arbuscular mycorrhizal (AM) fungi and nitrogen-fixing bacteria. InLotus japonicus, the latter occurs intracellularly by the cognate rhizobial partnerMesorhizobium lotior intercellularly with theAgrobacterium pusenseIRBG74 strain. Although these symbiotic programs show distinctive cellular and transcriptome signatures, some molecular components are shared. In this study, we demonstrate thatAldolase1, the first enzyme in the biosynthetic pathway of aromatic amino acids (AAA), plays a critical role in root hair development and for AM and rhizobial symbioses inLotus. Two homozygous mutants affected in Aldolase1 (ald1-1 andald1-2), show drastic alterations in the root hair morphology, associated with a progressive disruption of the actin cytoskeleton: a phenotype prevented through pharmacological and genetic complementation. Bothald1-1 andald1-2 show significant reductions in rhizobial infection (intracellular and intercellular), nodule organogenesis and AM colonization. RNAseq analysis ofald1-2roots suggested that these phenotypes are associated with downregulation of several cell wall related genes, and with an attenuated symbiotic signalling. This work provides robust evidence that links AAA metabolism to root hair development and successful symbiotic associations.

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Controlled Assays for Phenotyping the Effects of Strigolactone-Like Molecules on Arbuscular Mycorrhiza Development

Cited by 11 publications

References 38 publications

Resolving the haplotypes of arbuscular mycorrhizal fungi highlights the role of two nuclear populations in host interactions

Resolving the haplotypes of arbuscular mycorrhizal fungi highlights the role of two nuclear populations in host interactions

The wild grass Brachypodium distachyon as a developmental model system

Aromatic amino acid biosynthesis by aLotusAldolase impacts root hair development and symbiotic associations

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