Controlled presentation of recombinant proteins via a zinc-binding peptide-linker in two and three dimensional formats

Doran, Michael; Markway, Brandon D.; Croll, Tristan I.; Sergio, Sara; Munro, Trent P.; Cooper-White, Justin J.

doi:10.1016/j.biomaterials.2009.08.033

Cited by 11 publications

(9 citation statements)

References 26 publications

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“…Creation of a fusion protein containing the signaling protein along with binding motif [38,[206][207][208][209][210][211] The most likely reaction pathway of several available is shown, which involves ring expansion followed by reaction with an amine or other nucleophile. However, other reactions also occur which do not require a nucleophile to be present or do not involve ring expansion.…”

Section: Dienementioning

confidence: 99%

“…Hexahistidine (His6) motifs are commonly used in the purification of recombinant proteins, but can also be used to bind proteins onto cell culture substrates. Typically a surface activated with nickel-nitriloacetic acid (Ni-NTA) is used [37,241,242], although one study used zinc due to concerns with the potential cytotoxicity of nickel [206]. Dissociation constants down to 10 −13 have been reported [124].…”

Section: Affinity Interactions With Modified Ligandsmentioning

confidence: 99%

“…A final method of ligand co-presentation uses a protein fragment, such as the 9-10th [23,329] or 7-10th [22,142,206] type III domains of fibronectin, rather than short peptides. Consequently, this method requires recombinant production of the protein fragment, rather than the simpler solid-phase peptide synthesis, but is more likely to control both the spatial and orientational relationship between RGD and PHSRN and lead to higher ␣ 5 ␤ 1 integrin activation than the previous methods [22].…”

Section: Spatially Controlled Co-presentation Of Different Ligandsmentioning

confidence: 99%

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Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates

Rodda

Meagher

Nisbet

et al. 2014

Progress in Polymer Science

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Cells respond to their environment in complex and sometimes poorly understood ways. Protein, peptide and synthetic peptidomimetic ligands may all be used to stimulate cells via receptor signaling, using interactions that are often highly specific. Polymer substrates that present these ligands provide a promising way to control cell development, both for applications in biotechnology and for fundamental studies of cell biology. Here we review a large range of techniques that have been employed to create and characterize ligandfunctionalized substrates, with a particular focus on techniques that allow specific and consistent stimulation.

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Section: Dienementioning

confidence: 99%

Section: Affinity Interactions With Modified Ligandsmentioning

confidence: 99%

Section: Spatially Controlled Co-presentation Of Different Ligandsmentioning

confidence: 99%

See 1 more Smart Citation

Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates

Rodda

Meagher

Nisbet

et al. 2014

Progress in Polymer Science

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“…Recombinant proteins (eGFP and mCherry) or ECM peptides (fibronectin and vitronectin) were produced as previously described, 5,26 and sequences shown in Supplementary Table S2…”

Section: Synthesis Of Poly(nipam 43 )-Protein (Egfp Mcherry Fibronementioning

confidence: 99%

Thermoresponsive Worms for Expansion and Release of Human Embryonic Stem Cells

et al. 2014

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The development of robust suspension cultures of human embryonic stem cells (hESCs) without the use of cell membrane disrupting enzymes or inhibitors is critical for future clinical applications in regenerative medicine. We have achieved this by using long, flexible, and thermoresponsive polymer worms decorated with a recombinant vitronectin subdomain that bridge hESCs, aiding in hESC's natural ability to form embryoid bodies (EBs) and satisfying their inherent requirement for cell-cell and cell-extracellular matrix contact. When the EBs reached an optimal upper size where cytokine and nutrient penetration becomes limiting, these long and flexible polymer worms facilitated EB breakdown via a temperature shift from 37 to 25 °C. The thermoresponsive nature of the worms enabled a cyclical dissociation and propagation of the cells. Repeating the process for three cycles (over eighteen days) provided a >30-fold expansion in cell number while maintaining pluripotency, thereby providing a simple, nondestructive process for the 3D expansion of hESC.

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“…The method of biofunctionalisation depends on the property and chemistry of scaffolds and the nature of binding molecules. An appropriate presentation method should maximise the bioactivity of the scaffold 102,103 and minimise chronic inflammatory reactions after implantation. 27 For instance, covalently immobilised growth factors may amplify their trophic effect compared to soluble analouges due to multivalency, prevention of protein internalisation, as well as the comparatively high local concentrations of the growth factor on the scaffold surface compared to delivery in solution.…”

Section: Biofunctionalisation Of Scaffoldsmentioning

confidence: 99%

Biofunctionalisation of polymeric scaffolds for neural tissue engineering

et al. 2012

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Patients who experience injury to the central or peripheral nervous systems invariably suffer from a range of dysfunctions due to the limited ability for repair and reconstruction of damaged neural tissue. Whilst some treatment strategies can provide symptomatic improvement of motor and cognitive function, they fail to repair the injured circuits and rarely offer long-term disease modification. To this end, the biological molecules, used in combination with neural tissue engineering scaffolds, may provide feasible means to repair damaged neural pathways. This review will focus on three promising classes of neural tissue engineering scaffolds, namely hydrogels, electrospun nanofibres and self-assembling peptides. Additionally, the importance and methods for presenting biologically relevant molecules such as, neurotrophins, extracellular matrix proteins and protein-derived sequences that promote neuronal survival, proliferation and neurite outgrowth into the lesion will be discussed.

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Controlled presentation of recombinant proteins via a zinc-binding peptide-linker in two and three dimensional formats

Cited by 11 publications

References 26 publications

Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates

Specific control of cell–material interactions: Targeting cell receptors using ligand-functionalized polymer substrates

Thermoresponsive Worms for Expansion and Release of Human Embryonic Stem Cells

Biofunctionalisation of polymeric scaffolds for neural tissue engineering

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