Controlled release of nerve growth factor enhances sciatic nerve regeneration

Lee, Annie C; Yu, Vivian M.; Lowe, James B.; Brenner, Michael; Hunter, Daniel A.; Mackinnon, Susan E.; Sakiyama‐Elbert, Shelly E.

doi:10.1016/s0014-4886(03)00258-9

Cited by 348 publications

(278 citation statements)

References 30 publications

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“…Specifically, a longer gap length at longer survival times (Cai et al, 2004;Zhang et al, 2005;Haastert et al, 2006) with a different cell carrier and a known amount/duration of growth factor delivery must be studied and compared with the gold standard nerve autograft (Lee et al, 2003;Ahmed et al, 2005;Bellamkonda, 2006;Keilhoff et al, 2006). Although the nerve autograft still provides the best support for nerve regeneration followed by a conduit seeded with Schwann cells (Stang et al, 2005), both differentiated and undifferentiated MSCs are more supportive than a cell-free conduit (Tohill et al, 2004;Keilhoff et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Certain studies have reported that regeneration was improved when glial growth factor was added (Bryan et al, 2000), when the conduit covering the lesion gap was linked with brain-derived neurotrophic factor and ciliary neurotrophic factor (Ho et al, 1998), or when there is a slow release delivery of nerve growth factor (Lee et al, 2003;Chalfoun et al, 2006). Vascular endothelial growth factor (VEGF) may also improve nerve regeneration.…”

mentioning

confidence: 99%

“…The rationale is that Matrigel may provide a scaffold for axonal outgrowth and act as a matrix improving the retention of homologous bone marrow stem cells with or without VEGF transfection. An early time point of 21 days postoperative was selected for study to maximize the ability to detect group differences (Schroder et al, 1993;Lee et al, 2003;Mosahebi et al, 2003;Lundborg, 2004;Tohill et al, 2004).…”

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confidence: 99%

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Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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The early events associated with axonal growth into 10-mm nerve gaps were studied histologically in the rat sciatic nerve model to determine if the outgrowth of blood vessels, Schwann cells, and axons could be enhanced. In the first two experimental groups, collagen nerve guides were filled with either saline or Matrigel. Marrow-derived mesenchymal stem cells (MSCs) were added to Matrigel in two other groups, one of which contained cells transfected with VEGF (MSC/VEGF). After 21 days, the injury site was exposed, fixed, sectioned, and volume fractions of the conduit contents were determined by point counting. The bioresorbable collagen conduits appropriately guided the axons and vessels in a longitudinal direction. The volume fraction of axons was significantly greater in the group with saline when compared with all three groups with Matrigel. This measure had a significant positive correlation with actual counts of myelinated axons. The blood vessel volume fraction in the Matrigel group decreased compared with the saline group, but was restored in the MSC/VEGF group. All Matrigel groups had comparable cellularity and showed a distribution of residual Matrigel in acellular zones. The saline group, by contrast, sustained a network of delicate fibroblastic processes that compartmentalized the nerve and its natural matrix as it became infiltrated by axons as minifascicles. In conclusion, the reduction of axonal outgrowth in the Matrigel groups, when compared with the saline group, suggests that Matrigel may impede the early regenerative process even when enriched by the addition of MSCs or VEGF-transfected cells.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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“…The adaptability of hydrogel synthesis allows one to introduce signalling molecules via covalent linkage, non-covalent tethering or physical entrapment 42 , or as localized depots 40,41,43 , leading to spatial morphogen gradients that mimic a common paradigm in tissue development and regeneration. Non-covalent tethering in a hydrogel can be used as a mechanism to control diffusivity of general classes of growth factors such as heparin binders 44 , or specific growth factors such as nerve growth factor 45 .…”

Section: Hydrogels That Degrade With Timementioning

confidence: 99%

Moving from static to dynamic complexity in hydrogel design

2012

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Hydrogels are water-swollen polymer networks that have found a range of applications from biological scaffolds to contact lenses. Historically, their design has consisted primarily of static systems and those that exhibit simple degradation. However, advances in polymer synthesis and processing have led to a new generation of dynamic systems that are capable of responding to artificial triggers and biological signals with spatial precision. These systems will open up new possibilities for the use of hydrogels as model biological structures and in tissue regeneration.H ydrogels are water-swollen polymer networks that have been used for many decades, with applications as varied as contact lenses and super-absorbant materials. As the field of biomedical engineering has developed, hydrogels have become a prime candidate for application as molecule delivery vehicles and as carriers for cells in tissue engineering, owing to their ability to mimic many aspects of the native cellular environment (for example, high water content, mechanical properties that match soft tissues). Traditional hydrogels, formed through the covalent and non-covalent crosslinking of polymer chains, were regarded as relatively inert materials, providing a simple biomimetic three-dimensional (3D) environment, either for tissue production by local resident cells or for positioning of cells delivered in vivo. However, the simplicity of these materials may have in fact hindered their application, restricting cellular interactions with the environment and preventing uniform extracellular matrix (ECM) production and proper tissue development. In addition, these materials were limited to modelling static environments and lacked the spatiotemporal dynamic properties relevant for complex tissue processes. Fortunately, during the last decade the concepts of hydrogel design and cellular interaction have evolved, shedding light on how they may control cell behaviour, particularly for tissue engineering applications.Hydrogels with a range of mechanical properties, and capable of incorporating a wide range of biologically relevant molecules, from individual functional groups to multidomain proteins, are currently in development 1 . In addition, hydrogels are being designed with spatial heterogeneity, to either replicate properties in native tissue structures or to produce constructs with distinct regionally specific cell behavior 2 . As a result, studies to date have clearly demonstrated the possibility of creating well-defined microenvironments with control over the 3D presentation of signals to cells 3 . However, recently there has been a focus on the concept of hydrogels that exhibit dynamic complexity. These materials should evolve with time and in response to

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“…More recently, research has been focused mainly on improving the single lumen nerve tube to bridge larger nerve gaps (de Ruiter, Malessy, Yaszemski, Windebank, & Spinner, 2009;de Ruiter, Spinner, Yaszemski, Windebank, & Malessy, 2009). The artificial conduit may be implanted empty, or it may be filled with collagen and laminin-containing gels (Labrador, Buti, & Navarro, 1998;Madison, Da Silva, & Dikkes, 1988;Verdu et al, 2002), internal frameworks (de Ruiter, Spinner et al, 2009;Francel, Francel, Mackinnon, & Hertl, 1997;Lundborg & Kanje, 1996;Meek et al, 2001;Nakamura et al, 2004;Yoshii & Oka, 2001;Yoshii, Oka, Shima, Taniguchi, & Akagi, 2003), supportive cells (Ansselin, Fink, & Davey, 1997;Evans et al, 2002;Guenard, Kleitman, Morrissey, Bunge, & Aebischer, 1992;Kim et al, 1994;Rodriguez, Verdu, Ceballos, & Navarro, 2000;Sinis et al, 2005), growth factors (Derby et al, 1993;Fine, Decosterd, Papaloizos, Zurn, & Aebischer, 2002;Hollowell, Villadiego, & Rich, 1990;Lee et al, 2003;Midha, Munro, Dalton, Tator, & Shoichet, 2003;Sterne, Brown, Green, & Terenghi, 1997), and conductive polymers, but combinations have also already been used (Figure 2). An artificial graft can meet many of the needs of regenerating fibres by concentrating neurotrophic factors, reducing cellular invasion and providing directional neuritis outgrowth to prevent neuroma formation.…”

Section: Nrg1 To Promote Nerve Repair Peripheral Nerve Injury and Repairmentioning

confidence: 99%

Neuregulin 1 Role in Schwann Cell Regulation and Potential Applications to Promote Peripheral Nerve Regeneration

Gambarotta

Fregnan

Gnavi

et al. 2013

International Review of Neurobiology

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Neuregulin 1 (NRG1) is a multifunctional and versatile protein: its numerous isoforms can signal in a paracrine, autocrine or juxtacrine manner, playing a fundamental role during the development of the peripheral nervous system and during the process of nerve repair, suggesting that the treatment with NRG1 could improve functional outcome following injury. Accordingly, the use of NRG1 in vivo has already yielded encouraging results.The aim of this review is to focus on the role played by the different NRG1 isoforms during peripheral nerve regeneration and remyelination and to identify good candidates to be used for the development of tissue engineered medical devices delivering NRG1, with the final goal to promote better nerve repair.

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Controlled release of nerve growth factor enhances sciatic nerve regeneration

Cited by 348 publications

References 30 publications

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Moving from static to dynamic complexity in hydrogel design

Neuregulin 1 Role in Schwann Cell Regulation and Potential Applications to Promote Peripheral Nerve Regeneration

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