Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration

Chen, Xiaofeng; Liu, Yu; Miao, Leiying; Wang, Yangyang; Ren, Xiaobing; Yang, Xuebin; Hu, Yong; Sun, Weibin

doi:10.2147/ijn.s104324

Cited by 37 publications

(31 citation statements)

References 37 publications

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“…The interaction between BSA and metallic cations also resulted in a downshift of amide band I signal at 1646-1633 cm −1 as per HSAB Lewis's theory of binding (Bronze-Uhle et al, 2016). The formation of BSA-NF also led to the crystallization of phosphate ions from PBS and this was confirmed by the appearance of new peaks at 1033 and 561 cm −1 (Sun et al, 2016) (Figure S1a).…”

Section: Structural and Molecular Basis Of Selfassembled Bsa-nfmentioning

confidence: 72%

Reverse engineering of an anatomically equivalent nerve conduit

Ramesh

Dhandapani

Bagewadi

et al. 2021

J Tissue Eng Regen Med

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Reconstruction of peripheral nervous tissue remains challenging in critical-sized defects due to the lack of Büngner bands from the proximal to the distal nerve ends. Conventional nerve guides fail to bridge the large-sized defect owing to the formation of a thin fibrin cable. Hence, in the present study, an attempt was made to reverse engineer the intricate epi-, peri-and endo-neurial tissues using Fused Deposition Modeling based 3D printing. Bovine serum albumin protein nanoflowers (NF) exhibiting Viburnum opulus 'Roseum' morphology were ingrained into 3D printed constructs without affecting its secondary structure to enhance the axonal guidance from proximal to distal ends of denuded nerve ends. Scanning electron micrographs confirmed the uniform distribution of protein NF in 3D printed constructs. The PC-12 cells cultured on protein ingrained 3D printed scaffolds demonstrated cytocompatibility, improved cell adhesion and extended neuronal projections with significantly higher intensities of NF-200 and tubulin expressions.Further suture-free fixation designed in the current 3D printed construct aids facile implantation of printed conduits to the transected nerve ends. Hence the protein ingrained 3D printed construct would be a promising substitute to treat longer peripheral nerve defects as its structural equivalence of endo-and perineurial organization along with the ingrained protein NF promote the neuronal extension towards the distal ends by minimizing axonal dispersion.

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Section: Structural and Molecular Basis Of Selfassembled Bsa-nfmentioning

confidence: 72%

Reverse engineering of an anatomically equivalent nerve conduit

Ramesh

Dhandapani

Bagewadi

et al. 2021

J Tissue Eng Regen Med

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“…The application of this protein as an agent promoting cementum regeneration would also be interesting. The effects of bioresorbable scaffold loaded with rhCEMP-1 on the attachment, proliferation, and osteogenic and cementogenic differentiations of human periodontal ligament cells have been investigated histologically in a critical size defect on rodent, and the potential of generating cementum-like tissue in vitro and in vivo has been demonstrated [21].…”

Section: Discussionmentioning

confidence: 99%

CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial

Dellavia

Canciani

Rasperini

et al. 2019

Mediators of Inflammation

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Objectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair regeneration of this tissues. The aim of the present study was to assess if surgical procedures designed to regenerate periodontium might affect levels of cementum protein-1 (CEMP-1) in periodontal wound fluid during early phase of healing. Materials and Methods. In 36 patients, 18 intrabony periodontal defects were treated with regenerative therapy (REG group) and 18 suprabony periodontal defects were treated with open flap debridement (OFD group). In the experimental sites, gingival crevicular fluid was collected immediately before surgery, and periodontal wound fluid was collected 4, 7, 14, and 21 days after surgery. CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. At the analysis, it resulted that there was a significant average difference in CEMP-1 values between the REG and OFD groups at baseline (p=0.041), the CEMP-1-modeled average in the OFD group was lower by 0.45 ng/ml. There was a significant trend in CEMP-1 over time, and this trend was different among the 2 groups: the REG group showed a statistically significant rising CEMP-1 trend (0.18 ng/ml a week p=0.012), while the OFD had a trend that was significantly lower (-0.22 ng/ml a week compared to the REG group trend p=0.023), the OFD group lost on average 0.05 ng/ml a week. In REG sites, GCF protein levels resulted also related to clinical parameters. Conclusions. During the initial inflammatory phase of periodontal healing, CEMP-1 levels decrease regardless of the surgical protocol applied. The surgical procedures used to regenerate periodontal tissue are able to reverse this trend and to induce significant increase of CEMP-1 in periodontal wound fluid after the first week postop.

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“…Several approaches have been used and tested to regenerate teeth [ 67 ]; these approaches have focused on targeted regeneration of individual tooth components such as dental pulp and dentin [for reviews see 68 – 70 ; for functional studies see 70 – 73 ], cementum [for reviews see 74 , 75 ; for functional studies see 76 – 78 ], periodontium [for reviews see 79 , 80 ; for functional studies see 81 – 83 ], and enamel [for reviews see 84 ; for functional studies see 85 – 88 ]. However, combining these strategies does not guarantee the successful regeneration of a viable tooth.…”

Section: Overview Of Different Types Of Dental Stem Cellsmentioning

confidence: 99%

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

Baena

Casasco

Monti

2022

Stem Cell Rev and Rep

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One of the most exciting advances in life science research is the development of 3D cell culture systems to obtain complex structures called organoids and spheroids. These 3D cultures closely mimic in vivo conditions, where cells can grow and interact with their surroundings. This allows us to better study the spatio-temporal dynamics of organogenesis and organ function. Furthermore, physiologically relevant organoids cultures can be used for basic research, medical research, and drug discovery. Although most of the research thus far focuses on the development of heart, liver, kidney, and brain organoids, to name a few, most recently, these structures were obtained using dental stem cells to study in vitro tooth regeneration. This review aims to present the most up-to-date research showing how dental stem cells can be grown on specific biomaterials to induce their differentiation in 3D. The possibility of combining engineering and biology principles to replicate and/or increase tissue function has been an emerging and exciting field in medicine. The use of this methodology in dentistry has already yielded many interesting results paving the way for the improvement of dental care and successful therapies. Graphical abstract

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Controlled release of recombinant human cementum protein 1 from electrospun multiphasic scaffold for cementum regeneration

Cited by 37 publications

References 37 publications

Reverse engineering of an anatomically equivalent nerve conduit

Reverse engineering of an anatomically equivalent nerve conduit

CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial

Hypes and Hopes of Stem Cell Therapies in Dentistry: a Review

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