Controlled release of vascular endothelial growth factor enhances intestinal adaptation in rats with extensive small intestinal resection

Li, Nan; Ma, Garret; Zupekan, Tatiana; Stark, Rebecca; Puder, Mark; Dunn, James C.Y.

doi:10.1016/j.surg.2011.05.003

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…Some of these associations are supported by the published literature. As shown in Table 4, 8 of the top 20 significant biochemical/metabolic parameter-disease associations were verified by previous studies (Rasmuson et al, 2001; Lei et al, 2011; Sunose et al, 2011; Karki et al, 2015; Baldissarelli et al, 2016; Kim et al, 2017; Zhang Y. et al, 2018).…”

Section: Resultssupporting

confidence: 77%

Integrated Analysis of DNA Methylation and Biochemical/Metabolic Parameter During the Long-Term Isolation Environment

et al. 2019

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Numerous studies have shown that changes in the epigenome are an important cause of human biochemical or metabolic parameter changes. Biochemical/metabolic parameter disorders of the human body are usually closely related to the occurrence of disease. Therefore, constructing credible DNA methylation site-biochemical/metabolic parameter associations are key in interpreting the pathogenesis of diseases. However, there is a lack of research on systematic integration analysis of DNA methylation with biochemical/metabolic parameter and diseases. In this study, we attempted to use the four-people, multiple time point detected data from the long-term isolation experiment to conduct a correlation analysis. We used the biclustering algorithm FABIA to cluster the DNA methylation site-parameter correlation matrixes into 28 biclusters. The results of the biological function analysis for these biclusters were consistent with the biochemical/metabolic parameter change characteristics of the human body during long-term isolation, demonstrating the reliability of the biclusters identified by our method. In addition, from these biclusters, we obtained highly credible biochemical/metabolic parameter-disease associations, which is supported by several studies. Our results indicate that there is an overlap of biochemical/metabolic parameter-disease associations derived from a small sample, multiple time point data in healthy populations and the associations obtained from a large sample data in patients during disease development. These findings provide insights into understanding the role of the epigenome in biochemical/metabolic parameter change and disease development and has potential applications in biology and medicine research.

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Section: Resultssupporting

confidence: 77%

Integrated Analysis of DNA Methylation and Biochemical/Metabolic Parameter During the Long-Term Isolation Environment

et al. 2019

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“…GLP-2 functions to maintain the integrity of the intestinal epithelium through enhanced mesenteric blood flow, resulting in increased digestion and absorption of luminal nutrients from an enhanced absorptive surface area (6,11,18,19,30,53). The mechanism by which GLP-2 functions still remains unknown, but promising work has demonstrated multiple downstream mediators of action, including insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), vascular endothelial growth factor, keratinocyte growth factor, nitric oxide, vasoactive intestinal peptide, and ErbB ligands (12,15,18,19,27,30,35,36,49,50). Phase III clinical trials have recently demonstrated that, in a randomized placebo-controlled trial, patients given teduglutide, a DPP-IV-resistant GLP-2 analog, had a statistically significant decrease in parenteral nutrition (PN) volume and in some cases were liberated from PN (21).…”

mentioning

confidence: 99%

Enteral nutrients potentiate glucagon-like peptide-2 action and reduce dependence on parenteral nutrition in a rat model of human intestinal failure

Brinkman

Murali

Hitt

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Glucagon-like peptide-2 (GLP-2) is a nutrient-dependent, proglucagon-derived gut hormone that shows promise for the treatment of short bowel syndrome (SBS). Our objective was to investigate how combination GLP-2 + enteral nutrients (EN) affects intestinal adaption in a rat model that mimics severe human SBS and requires parenteral nutrition (PN). Male Sprague-Dawley rats were assigned to one of five groups and maintained with PN for 18 days: total parenteral nutrition (TPN) alone, TPN + GLP-2 (100 μg·kg(-1)·day(-1)), PN + EN + GLP-2(7 days), PN + EN + GLP-2(18 days), and a nonsurgical oral reference group. Animals underwent massive distal bowel resection followed by jejunocolic anastomosis and placement of jugular catheters. Starting on postoperative day 4, rats in the EN groups were allowed ad libitum access to EN. Groups provided PN + EN + GLP-2 had their rate of PN reduced by 0.25 ml/day starting on postoperative day 6. Groups provided PN + EN + GLP-2 demonstrated significantly greater body weight gain with similar energy intake and a safe 80% reduction in PN compared with TPN ± GLP-2. Groups provided PN + EN + GLP-2 for 7 or 18 days showed similar body weight gain, residual jejunal length, and digestive capacity. Groups provided PN + EN + GLP-2 showed increased jejunal GLP-2 receptor (GLP-2R), insulin-like growth factor-I (IGF-I), and IGF-binding protein-5 (IGFBP-5) expression. Treatment with TPN + GLP-2 demonstrated increased jejunal expression of epidermal growth factor. Cessation of GLP-2 after 7 days with continued EN sustained the majority of intestinal adaption and significantly increased expression of colonic proglucagon compared with PN + EN + GLP-2 for 18 days, and increased plasma GLP-2 concentrations compared with TPN alone. In summary, EN potentiate the intestinotrophic actions of GLP-2 by improving body weight gain allowing for a safe 80% reduction in PN with increased jejunal expression of GLP-2R, IGF-I, and IGFBP-5 following distal bowel resection in the rat.

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“…Animal experiments were conducted with the approval of the local ethic committee (Luebeck University, V 312-72241.122-24 (43-3/06)) on adult Wistar rats (260–360 g). Twelve rats underwent a massive resection of the small intestine to induce SBS (rSBS) as described by Lei et al ( 2011 ). Rats were anesthetized with an intraperitoneal injection of ketamine (40 mg/kg) and Rompun (4 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

Muscle hypertrophy and neuroplasticity in the small bowel in short bowel syndrome

Khasanov

Svoboda

Tapia-Laliena

et al. 2023

Histochem Cell Biol

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Short bowel syndrome (SBS) is a severe, life-threatening condition and one of the leading causes of intestinal failure in children. Here we were interested in changes in muscle layers and especially in the myenteric plexus of the enteric nervous system (ENS) of the small bowel in the context of intestinal adaptation. Twelve rats underwent a massive resection of the small intestine to induce SBS. Sham laparotomy without small bowel transection was performed in 10 rats. Two weeks after surgery, the remaining jejunum and ileum were harvested and studied. Samples of human small bowel were obtained from patients who underwent resection of small bowel segments due to a medical indication. Morphological changes in the muscle layers and the expression of nestin, a marker for neuronal plasticity, were studied. Following SBS, muscle tissue increases significantly in both parts of the small bowel, i.e., jejunum and ileum. The leading pathophysiological mechanism of these changes is hypertrophy. Additionally, we observed an increased nestin expression in the myenteric plexus in the remaining bowel with SBS. Our human data also showed that in patients with SBS, the proportion of stem cells in the myenteric plexus had risen by more than twofold. Our findings suggest that the ENS is tightly connected to changes in intestinal muscle layers and is critically involved in the process of intestinal adaptation to SBS.

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Controlled release of vascular endothelial growth factor enhances intestinal adaptation in rats with extensive small intestinal resection

Cited by 9 publications

References 19 publications

Integrated Analysis of DNA Methylation and Biochemical/Metabolic Parameter During the Long-Term Isolation Environment

Integrated Analysis of DNA Methylation and Biochemical/Metabolic Parameter During the Long-Term Isolation Environment

Enteral nutrients potentiate glucagon-like peptide-2 action and reduce dependence on parenteral nutrition in a rat model of human intestinal failure

Muscle hypertrophy and neuroplasticity in the small bowel in short bowel syndrome

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