Controlled Transport of Magnetic Particles and Cells Using C-Shaped Magnetic Thin Films in Microfluidic Chips

Abstract

Single-cell analysis is an emerging discipline that has shown a transformative impact in cell biology in the last decade. Progress in this field requires systems capable of accurately moving the cells and particles in a controlled manner. Here, we present a microfluidic platform equipped with C-shaped magnetic thin films to precisely transport magnetic particles in a tri-axial rotating magnetic field. This innovative system, compared to the other rivals, offers numerous advantages. The magnetic particles repel each other to prevent undesired cluster formation. Many particles move synced with the external rotating magnetic field, which results in highly parallel controlled particle transport. We show that the particle transport in this system is analogous to electron transport and Ohm’s law in electrical circuits. The proposed magnetic transport pattern is carefully studied using both simulations and experiments for various parameters, including the magnetic field characteristics, particle size, and gap size in the design. We demonstrate the appropriate transport of both magnetic beads and magnetized living cells. We also show a pilot mRNA-capturing experiment with barcode-carrying magnetic beads. The introduced chip offers fundamental potential applications in the fields of single-cell biology and bioengineering.

A Microfluidic Chip for Single-Cell Capture Based on Stagnation Point Flow and Boundary Effects

“…Yu et al used a microfluidic chip bonded with PDMS and glass to perform biochemical stimulation on single cells [15]. In previous investigations, diverse methodologies have been proposed for capturing single cells within microfluidic chips, including electrodynamic trapping [16], magnetic trapping [17], optical trapping [18], and hydrodynamic capture [19]. These methodologies have been extensively employed to explore an array of both physical and biological phenomena.…”