Controlling enzyme function through immobilisation on graphene, graphene derivatives and other two dimensional nanomaterials

Abstract

Controlling enzyme function through immobilisation on graphene, graphene derivatives and other two dimensional nanomaterials.

Synthesis of lutein esters using a novel biocatalyst of Candida antarctica lipase B covalently immobilized on functionalized graphitic carbon nitride nanosheets

“…As an essential participator in the immobilization process, supporting material deeply affects lipase activity. Two-dimensional nanomaterials such as graphene oxide 18 and carbon nanosheets 19,20 have gained extensive attention 21 due to their large surface area, abundant active sites, and good biocompatibility. As a superior material, 2D carbon nitride nanosheets exhibit excellent performance in many elds.…”

From Graphite to Laccase Biofunctionalized Few-Layer Graphene: A “One Pot” Approach Using a Chimeric Enzyme

“…Like other nanomaterials, graphene is a very suitable platform for enzyme immobilization thanks to its high surface area, dispersion in solution, and tunable surface chemistry. Intense efforts have been devoted to this research field in the last five years, resulting in the immobilization of different enzymes for various applications [18]. However, the hydrophobic interactions driving the direct immobilization of active proteins on graphene surface are often difficult to achieve, and modifications of the protein 3D structures can occur with detrimental effects on their functionality [19].…”

“…However, the hydrophobic interactions driving the direct immobilization of active proteins on graphene surface are often difficult to achieve, and modifications of the protein 3D structures can occur with detrimental effects on their functionality [19]. Two main approaches have been implemented, and often combined, to overcome this issue, such as the use of graphene oxide (GO) whose surface is more hydrophilic, or the exploitation of graphene-based composites, such as microcellulose, chitosan, and various metal oxides [18].…”

Recombinant Escherichia coli BL21-pET28a-egfp Cultivated with Nanomaterials in a Modified Microchannel for Biofilm Formation

“…Carbon nanomaterials, as a new type of highly efficient adsorption carrier, have the advantages of larger specific surface area, higher loading capacity, ordered nanopore structure, excellent chemical stability, and biocompatibility [ 17 ]. Carbon nanomaterials such as carbon nanotubes and graphene have attracted widespread attention in the field of biocatalysis [ 18 ]. Multiwalled carbon nanotubes were used for the immobilization of lipase in the conversion of Jatropha oil to fatty acid methyl esters, which showed excellent adsorption capacity and biocompatibility [ 19 ].…”