Controlling of growth performance, lipid deposits and fatty acid composition of chicken meat through a probiotic, Lactobacillus johnsonii during subclinical Clostridium perfringens infection

Wang, Hesong; Liu, Lei; Zeng, Dong; Lai, Jing; Qing, Xiaodan; Li, Guangyao; Pan, Kangcheng; Jing, Bo

doi:10.1186/s12944-017-0408-7

Cited by 37 publications

(33 citation statements)

References 48 publications

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“…38 Infected broilers with CP showed non-significant decrease in total lipid, cholesterol and triglyceride levels throughout the experimental period after infection, while treatment of infected broilers by bacitracin and/or lincomycin restored the lipid profile compared with control broilers. Similar results were recorded previously in broilers 39 and in turkeys 40 suffering from NE.…”

Section: Discussionsupporting

confidence: 91%

“…Infected broilers with CP resulted in weakening the antioxidant defense mechanism as evidenced by decreases in SOD and catalase associated with an elevation in MDA. 39 It could be concluded that the combination of lincomycin and bacitracin at the tested doses was of considerable value for the control of NE in broiler chickens than using them separately.…”

Section: Discussionmentioning

confidence: 94%

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Comparative studies on the efficacy of lincomycin and bacitracin for the control of necrotic enteritis in broiler chickens

Elkomy¹,

Farag²,

Gharbawy³

et al. 2019

Int J Basic Clin Pharmacol

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Background: The present study was conducted to evaluate the efficacy of lincomycin and/or bacitracin for control of experimentally-induced Clostridium perfringens (CP) infection in broiler chickens.Methods: A total of 100 one-day-old Cobb-mixed chicks were divided into five groups (A, B, C, D and E, each of 20 bird). At the 15th day of age, all birds (except group A) were inoculated orally with CP broth culture (109 CFU/mL). Two days later, drugs were orally administered once daily for five consecutive days as follow; Group A and B were left untreated. Group C, D, and E were treated with lincomycin (0.5 g/l), bacitracin (100 mg/l), lincomycin and bacitracin, respectively. The efficacy of used drugs was estimated based on clinical symptoms, body weight, weight gain, feed conversion rate. Hematobiochemical changes were also determined.Results: Necrotic enteritis in broiler chickens induced a significant decrease in body weight, weight gain, erythrocytic count, hemoglobin content, PCV %, serum proteins, catalase, and superoxide dismutase. Additionally, a marked decrease in serum lipids was obtained. Furthermore, a significant increase in feed conversion rate, leukocytic count, phagocytic activity, phagocytic index, serum total globulin, γ globulin and malondialdehyde coupled with a marked increase in β and α globulins were determined. Medication of infected broilers with lincomycin and/or bacitracin improved clinical signs and reduced mortality rate to 8, 6 and 2%, respectively, as well as restored the performance and hematobiochemical alterations.Conclusions: a combination of lincomycin and bacitracin was of considerable value for the control of necrotic enteritis in broiler chickens.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 94%

Comparative studies on the efficacy of lincomycin and bacitracin for the control of necrotic enteritis in broiler chickens

Elkomy¹,

Farag²,

Gharbawy³

et al. 2019

Int J Basic Clin Pharmacol

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“…The correlation between increased IL-17A expression and L. johnsonii abundance parallels the intestinal induction of IL-17A observed in response to tightly adherent symbiotic species (60)(61)(62)(63)(64). L. johnsonii is an established probiotic that has been demonstrated to have beneficial effects when applied in poultry production (48)(49)(50)(51)(52)(53). Several modes of action have been proposed for probiotic strains of L. johnsonii but underlying these is the multi-modal ability of the species to affect epithelial gut cell adherence (65)(66)(67)(68), which we propose will induce the expression of IL-17A.…”

Section: Resultsmentioning

confidence: 69%

“…In broiler chickens, administration of a L. johnsonii isolate has been reported to improve growth performance (48). Subsequently it was reported that meat from L. johnsonii treated birds had higher nutritional value and the birds showed resistance to the development of necrotic enteritis (49,50). Similar results were obtained by administering L. johnsonii LB1 to piglets to improve performance and reduce diarrhea (51).…”

Section: Discussionmentioning

confidence: 77%

Galacto-oligosaccharides modulate the juvenile gut microbiome and innate immunity to improve broiler chicken performance

Richards

Lafontaine

Connerton

et al. 2019

Preprint

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Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides in broiler feed enhanced the growth rate and feed conversion of chickens relative to a calorie-matched control diet. Comparison of the cecal microbiota identified key differences in abundance of Lactobacillus spp. Increased levels of L. johnsonii in GOS-fed juvenile birds at the expense of L. crispatus was linked to improved performance (growth rate and market weight). Investigation of the innate immune responses highlighted increases of ileal and cecal IL-17A gene expression counterposed to a decrease in IL-10 and IL-17F. Quantification of the autochthonous Lactobacillus ssp. revealed a correlation between bird performance and L. johnsonii abundance. Shifts in the cecal populations of key Lactobacillus spp. of juvenile birds primed intestinal innate immunity without harmful pathogen challenge. IMPORTANCEImprovements in the growth rate of broiler chickens can be achieved through dietary manipulation of the naturally occurring bacterial populations whilst mitigating the withdrawal of antibiotic growth promoters. Prebiotic galacto-oligosaccharides (GOS) are manufactured as a by-product of dairy cheese production, which can be incorporated in the diets of juvenile chickens to improve their health and performance. This study investigates the key mechanisms behind this progression and pin points L. johnsonii as a key species that facilitates the enhancements in growth rate and gut health. It also relates the role of the innate immune system in the response to the GOS diet.in the PCR reaction and converted into genome copy number per gram of cecal content based on the mass of cecal material and elution volume applied for DNA extraction.Data and Statistical Analysis. For the microbiota -diversity analysis Bray Curtis distances were tested for significance using AMOVA implemented within Mothur (79). Linear discriminant analysis effect size (LEfSe) was used to identify differentially abundant OTUs (82) and implemented within Mothur (79). Data processing and ordination were performed using R (R Development Core Team, 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0; URL http://www.R-project.org ; 83). With the exception of Figure S2 all figures were drawn using R version 3.5.3 (2019-03-11) in Rstudio 1.1 (84,85). R scripts used to draw figures are available at: https://github.com/PJRichards/Richards_GOS_broiler. Significance tests of heterophil counts, villus and crypt measurements were performed using single-factor ANOVA with p < 0.05 used as the level significance. Non-normally distributed gene expression data were compared using the non-parametric Kruskal-Wallis test. Data Availability. The genome DNA sequences of L. crispatus DC21.1 appear in the NCBI database under the accession numbers CP039266-CP039267.The genome DNA sequences L. johnsonii DC22.2 appear in the NCBI database under the access...

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“…As chicks show no clinical sign of the disease, most economic losses result from the presence of subclinical forms ( 3 , 4 ). Aggravating the condition, meat containing affected nutrients or toxins may be purchased and consumed by customers ( 5 ). Therefore, an effective alternative to antibiotics must be developed for the prevention of SNE in broilers.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Enhanced Intestinal Immunity in Broilers against Subclinical Necrotic Enteritis

Wang

Qing

et al. 2017

Front. Immunol.

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Along with banning of antibiotics, necrotic enteritis (NE), especially subclinical NE (SNE) whereby no clinical signs are present in chicks, has become one of the most threatening problems in poultry industry. Therefore, increasing attention has been focused on research and application of effective probiotic strains, as an alternative to antibiotics, to prevent SNE in broilers. In the present study, we evaluated the effects of Lactobacillus johnsonii BS15 on the prevention of SNE in broilers. Specifically, assessment determined the growth performance and indexes related to intestinal mucosal immunity in the ileum and cecal tonsil of broilers. A total of 300 1-day-old Cobb 500 chicks were randomly distributed into the following 5 groups: control group (fed with basal diet + de Man, Rogosa, and Sharpe liquid medium [normal diet]), SNE group (normal diet), BS15 group (basal diet + 1 × 106 colony-forming units BS15/g as fed [BS15 diet]), treatment group (normal diet [days 1–28] + BS15 diet [days 29–42]), and prevention group (BS15 diet [days 1–28] + normal diet [days 29–42]) throughout a 42-day experimental period. SNE infection was treated for all chicks in the SNE, BS15, treatment, and prevention groups. The present results demonstrated that BS15 supplementation of feeds in BS15 and prevention groups exerted a positive effect on preventing negative influences on growth performance; these negative influences included low body weight gain and increased feed conversion ratio caused by SNE. Although no changes were detected in all determined indexes in cecal tonsils, BS15-treated broilers were free from SNE-caused damage in villi in the ileum. BS15 inhibited SNE-caused decrease in immunoglobulins in the ileum. In the lamina propria of ileum, T cell subsets of lymphocytes influenced by SNE were also controlled by BS15. BS15 affected antioxidant abilities of the ileum and controlled SNE-induced mitochondrion-mediated apoptosis by positively changing contents and/or mRNA expression levels of apoptosis-related proteins. These findings indicate that BS15 supplementation may prevent SNE-affected growth decline mainly through enhancing intestinal immunity in broilers.

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Controlling of growth performance, lipid deposits and fatty acid composition of chicken meat through a probiotic, Lactobacillus johnsonii during subclinical Clostridium perfringens infection

Cited by 37 publications

References 48 publications

Comparative studies on the efficacy of lincomycin and bacitracin for the control of necrotic enteritis in broiler chickens

Comparative studies on the efficacy of lincomycin and bacitracin for the control of necrotic enteritis in broiler chickens

Galacto-oligosaccharides modulate the juvenile gut microbiome and innate immunity to improve broiler chicken performance

Probiotic Enhanced Intestinal Immunity in Broilers against Subclinical Necrotic Enteritis

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