Controlling of microbial biofilms formation: Anti- and probiofilm agents

Плакунов, В. К.; Mart’yanov, S. V.; Teteneva, N. A.; Журина, М. В.

doi:10.1134/s0026261717040129

Cited by 27 publications

(8 citation statements)

References 171 publications

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“…Hormones and other humoral regulators without direct antimicrobial effects have been suggested to be useful as components of future complex antimicrobials, where they could serve as pathogen attenuators. For instance, local application of such an agent in an area of skin inflammation in combination with an antibiotic could increase the antimicrobial effect [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Ганнесен

Schelkunov

Geras’kina

et al. 2021

Biofilm

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In this study, the effect of epinephrine on the biofilm formation of Micrococcus luteus C01 isolated from human skin was investigated in depth for the first time. This hormone has a complex effect on biofilms in various systems. In a system with polytetrafluoroethylene (PTFE) cubes, treatment with epinephrine at a physiological concentration of 4.9 × 10 −9 M increased the total amount of 72-h biofilm biomass stained with crystal violet and increased the metabolic activity of biofilms, but at higher and lower concentrations, the treatment had no significant effect. On glass fiber filters, treatment with the hormone decreased the number of colony forming units (CFUs) and changed the aggregation but did not affect the metabolic activity of biofilm cells. In glass bottom plates examined by confocal microscopy, epinephrine notably inhibited the growth of biofilms. RNA-seq analysis and RT–PCR demonstrated reproducible upregulation of genes encoding Fe–S cluster assembly factors and cyanide detoxification sulfurtransferase, whereas genes encoding the co-chaperone GroES, the LysE superfamily of lysine exporters, short-chain alcohol dehydrogenase and the potential c-di-GMP phosphotransferase were downregulated. Our results suggest that epinephrine may stimulate matrix synthesis in M. luteus biofilms, thereby increasing the activity of NAD(H) oxidoreductases. Potential c-di-GMP pathway proteins are essential in these processes.

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Section: Introductionmentioning

confidence: 99%

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Ганнесен

Schelkunov

Geras’kina

et al. 2021

Biofilm

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“…Most studies dedicated to discovering the new antibiofilm drugs are aimed on specific targeting of biofilm-related pathways (8)(9)(10). While this hypothesis-guided approach is potentially promising, it is limited by our poor understanding of the complexity of on November 1, 2020 by guest http://aem.asm.org/ Downloaded from regulation that underlies transition to the biofilm state.…”

Section: Discussionmentioning

confidence: 99%

“…Since cells within biofilms have low sensitivity to many antimicrobial compounds (7), prevention of biofilm formation is important in disease treatment. A number of different alternative approaches are currently explored as the antibiofilm strategies, including surface modification to prevent bacterial adhesion or assembly of the biofilm matrix, specific enzymes to degrade biofilm matrix, and inhibition of bacterial signaling or quorum sensing using small molecules (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Multiple Drug-Induced Stress Responses Inhibit Formation of Escherichia coli Biofilms

Teteneva

Mart’yanov

Esteban-López

et al. 2020

Appl Environ Microbiol

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In most ecosystems, bacteria primarily exist as structured surface-associated biofilms that can be highly tolerant to antibiotics and thus represent an important health issue. Here we explored drug repurposing as a strategy to identify new antibiofilm compounds, screening over 1000 compounds from the Prestwick Chemical Library of approved drugs for specific activities that prevent biofilm formation by Escherichia coli. Most growth-inhibiting compounds, which include known antibacterial but also antiviral and other drugs, had also reduced biofilm formation. However, we also identified several drugs that were biofilm-inhibitory at doses where only weak or no effect on planktonic growth could be observed. Activities of the most specific antibiofilm compounds were further characterized using gene expression analysis, proteomics and microscopy. We observed that most of these drugs acted by repressing genes responsible for production of curli, major component of E. coli biofilm matrix. This repression apparently occurred through induction of several different stress responses, including DNA and cell-wall damage, and homeostasis of divalent cations, demonstrating that biofilm formation can be inhibited through a variety of molecular mechanisms. One tested drug, tyloxapol, inhibited biofilm formation independent of curli expression or growth, by suppressing bacterial attachment at the surface. Importance Prevention of bacterial biofilm formation is one of the major current challenges in microbiology. By systematically screening a large number of approved drugs for their ability to suppress biofilm formation by Escherichia coli, here we identified a number of prospective antibiofilm compounds. We further demonstrated different mechanisms of action for individual compounds, from induction of replicative stress to disbalance of cation homeostasis to inhibition of bacterial attachment to the surface. Our work demonstrates the potential of drug repurposing for the prevention of bacterial biofilm formation and suggests that also for other bacteria the activity spectrum of antibiofilm compounds is likely to be broad.

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“…Survival of microbial populations in the environment depends on the adequacy of the microbial cell metabolism, which is controlled by regulatory systems, environmental conditions, determining homeostasis of a cell, and the population in general. The main form of microbial existence in the natural systems is their growth in biofilms (BFs) [1][2][3]. Biofilms are mono-species or multi-species communities embedded into the polymeric material-matrix produced by microorganisms themselves.…”

Section: Introductionmentioning

confidence: 99%

“…Methods for sanitary treatment applied in food enterprises do not ensure a sufficient level of sterility and, thus, microbiological safety [9][10][11][12]. This situation is explained by the high resistance of BFs to damaging impacts, which is associated with: (1) the protective function of the biofilm matrix [13,14]; (2) increased resistance of the biofilm phenotype of cells in the BF composition [15,16]; (3) an increase in the resistance of cells in multi-species BFs compared to mono-species [6,7,[17][18][19][20]; and (4) age-related heterogeneity of the biofilm population, where old cells constitutively have higher adaptive resistance to sterilization means and other damaging agents than younger cells [12,21,22]. However, the question of physiological heterogeneity of cells in BFs, that is, the presence, proportion, and balance of different cell types (active cells, persisters, and dormant forms), is not investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

Forms of Bacterial Survival in Model Biofilms

et al. 2022

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Bacterial survival upon sharp fluctuations of environmental parameters and exposure to lethal doses of stressors (antibiotics, disinfectants, heat shock, and others) is ensured by the use of different strategies of resistance, an important place among which is occupied by the forms with reduced or stopped metabolism, antibiotic tolerant (AT) persister (P) cells, anabiotic dormant forms (DFs), and viable but non-culturable (VBNC) cells. Elucidating the role of these forms of bacterial resistance to an impact of chemical and biological toxicants and physical stressors is of great fundamental and practical interest. The aim of this research was to study the dynamics of the resistance forms in bacteria developing in biofilms and, for comparison in liquid media, upon exposure to lethal doses of antibiotics and heat shock (80 °C, 15 min). In the trials, the experimental model of the development of monospecies and binary forms of bacterial biofilms including contaminants of meat products (eight strains of genera Pseudomonas, Escherichia, Salmonella, Staphylococcus, and Kocuria) on the fiberglass filters was used. It was established that survival of populations in the presence of lethal doses of antibiotics and upon heating was ensured by persister cells forming in bacterial populations and, at the late stages of the development of biofilm or planktonic cultures (28 days of incubation), by anabiotic DFs. With that, the number of thermoresistant (TR) DFs (103–104 CFU/mL) in dying biofilms (28 days) developing in the standard conditions (composition and volume of a medium, pH, growth temperature) weakly depended on the bacterial taxonomic status. This study demonstrates the heterogeneity of DF populations of biofilm bacterial cultures in terms of the depth of dormancy, as a result of which the number of thermoresistant DFs after heating can exceed their total number before heating (due to the effect of DF revival, resuscitation). When studying the dynamics of TR cells and P cells in bacterial biofilm and planktonic cultures, it was found that their number (CFU/mL) in populations decreased up to the absence of TR cells and P cells on the 21st day of growth and was restored on the 28th day of growth. The revealed phenomenon can be explained only by cardinal changes in the ultrastructural organization of cells, namely, cytoplasm vitrification due to a sharp decrease in an amount of free water in a cell, which, according to the results obtained, occurs in the period between the 21st and 28th days of incubation. A high degree of correlation between the number of AT cells and TR cells (0.5–0.92) confirms the hypothesis that regards P cells as precursors of DFs.

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Controlling of microbial biofilms formation: Anti- and probiofilm agents

Cited by 27 publications

References 171 publications

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Epinephrine affects gene expression levels and has a complex effect on biofilm formation in Micrococcus luteus strain C01 isolated from human skin

Multiple Drug-Induced Stress Responses Inhibit Formation of Escherichia coli Biofilms

Forms of Bacterial Survival in Model Biofilms

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