Mustopa AZ, Puspitasari IF, Fatimah, Triratna L, Kartina G. 2018. Genetic diversity of mastitis cow’s milk bacteria based onRAPD-PCR. Biodiversitas 19: 1714-1721. Mastitis in cow is caused by several pathogenic bacteria, including antibiotic-resistantbacteria. Identification of the pathogenic bacteria’s diversity that is contaminating cow’s milk needs to be done. The aim of this researchwas to conduct molecular identification in mastitis cow’s milk bacteria through RAPD-PCR (Random Amplified Polymorphism DNAPolymeraseChain Reaction) analysis. Bacteria from mastitis cow’s milk were enumerated using selective media. Based on the result ofmedia selection, there were 72 isolates of bacteria from mastitis cows in Ciguha, Guranteng, Cikarenceng Village (Pagerageung,Tasikmalaya), and Warnasari Village (Pangalengan, Bandung), West Java, Indonesia. The genomes from these isolates were extractedand then subjected to RAPD-PCR analysis. The results of RAPD-PCR analysis showed 8 clusters of dendrogram which 4 dominantclusters were selected. Identification of 4 dominant clusters, which contained representative strains, using 16s rRNA showed the isolatesBPA-12, MHA-6, L-4, and XLDA-8 were identified as Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, andEnterobacter bugandensis.