Controlling the simultaneous production of laccase and lignin peroxidase from Streptomyces cinnamomensis by medium formulation

Abstract

BackgroundUse of crude ligninase of bacterial origin is one of the most promising ways to improve the practical biodegradation of lignocellulosic biomass. However, lignin is composed of diverse monolignols with different abundance levels in different plant biomass and requires different proportions of ligninase to realize efficient degradation. To improve activity and reduce cost, the simultaneous submerged fermentation of laccase and lignin peroxidase (LiP) from a new bacterial strain, Streptomyces cinnamomensis, was studied by adopting formulation design, principal component analysis, regression analysis and unconstrained mathematical programming.ResultsThe activities of laccase and LiP from S. cinnamomensis cultured with the optimal medium formulations were improved to be five to eight folders of their initial activities, and the measured laccase:LiP activity ratios reached 0.1, 0.4 and 1.7 when cultured on medium with formulations designed to produce laccase:LiP complexes with theoretical laccase:LiP activity ratios of 0.05 to 0.1, 0.5 to 1 and 1.1 to 2.ConclusionBoth the laccase and LiP activities and also the activity ratio of laccase to LiP could be controlled by the medium formulation as designed. Using a crude laccase-LiP complex with a specially designed laccase:LiP activity ratio has the potential to improve the degradation of various plant lignins composed of diverse monolignols with different abundance levels.

Application of Central Composite Designs Utilized for Scale-up Strategies in the Optimization Process for Development Laccase Production and for the Bioremediation of Various Industrial Pollutants

“…The media supplement with corn cob and sugarcane pith characterized by tiny induction effect, where laccase level slightly climbed up by 7.9 and 6.6% more than control trial. However, the rice straw and rice bran had not significant effect on laccase production; about 1.9 and 0.9% more increase than the control trial with observations reported by other investigators [9,17,25] who found that rice straw, wheat bran, peanut shell, banana peel, and black grapes were identified as promising substrates for laccase production, while the other substrates like corn cops, rice straw, and sugarcane bagasse caused low laccase production. However, the observation documented by other investigators [26,27] are compatible with the results of this study in term of apple and wheat bran caused decline in laccase production.…”

Actinomycetes: A Source of Lignocellulolytic Enzymes

“…isolated from termite Amitermes hastatus have indicated production of laccases, lignin peroxidases, or manganese peroxidases enzymes by them [ 118 ]. Laccase and lignin peroxidase activities have also been observed in Streptomyces cinnamomeus [ 119 ]. Laccase enzyme studies were carried out including their structural elucidation in Streptomyces lavendulae , Streptomyces psammoticus , Streptomyces ipomoeae , and Streptomyces sviceus [ 61 ].…”

Assessing water quality by ratio of the number of dominant bacterium species between surface/subsurface sediments in Haihe River Basin

“…), and obligate anaerobic species (such as Clostridium sp.) have been identified as the dominant species (Brück et al, 2010;Jing, 2010;Jing and Wang, 2012). Although the difference in microbial community composition can be observed in electrophoretic profiles obtained by DGGE (Kent and Triplett, 2002), no definite correlation was found between the diversity of bacterial communities (Supplementary Data 3-a-e) and the comprehensive G average (Supplementary Data 4-a-d).…”