Contusion Models of Spinal Cord Injury in Rats

Dunham, Kelly A.; Floyd, Candace L.

doi:10.1007/978-1-61779-301-1_18

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Based on the fact that any therapeutic strategy in the treatment of SCI has to be examined in animal models that closely mimic the human SCI condition, currently there are various models that all have aimed to recreate features of human SCI (Dunham and Floyd, 2011;Zhang et al, 2014). The in vitro model of injury in spi nal cord organotypic slice culture is one of the models that has been identified as a robust approach for SCI-related investigations (Pandamooz et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Epidermal Neural Crest Stem Cells in Organotypic Spinal Cord Slice Culture Platform

Pandamooz,

Saied,

Nabiuni

et al. 2016

Fol. Biol.

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Among various strategies employed for spinal cord injury, stem cell therapy is a potential treatment. So far, a variety of stem cells have been evaluated in animal models and humans with spinal cord injury, and epidermal neural crest stem cells represent one of the attractive types in this area. Although these multipotent stem cells have been assessed in several spinal cord injury models by independent laboratories, extensive work remains to be done to ascertain whether these cells can safely improve the outcome following human spinal cord injury. Among the models that closely mimic human spinal cord injury, the in vitro model of injury in organotypic spinal cord slice culture has been identified as one of the faithful platforms for injury-related investigations. In this study, green fluorescent protein-expressing stem cells were grafted into injured organotypic spinal cord slice culture and their survival was examined by confocal microscope seven days after transplantation. Data obtained from this preliminary study showed that these stem cells can survive on top of the surface of injured slices, as observed on day seven following their transplantation. This result revealed that this in vitro model of injury can be considered as a suitable context for further evaluation of epidermal neural crest stem cells before their application in large animals.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Epidermal Neural Crest Stem Cells in Organotypic Spinal Cord Slice Culture Platform

Pandamooz,

Saied,

Nabiuni

et al. 2016

Fol. Biol.

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“…TARLOV et al (1953) introduziram a técnica de compressão medular em cães por implantação de um balão infl ável no espaço extradural. O desenvolvimento de balões infl áveis menores tornou possível estudar tais modelos em animais de laboratório (RIVLIN & TATOR, 1978). Guner & Young em 1992 desenvolveram o Impactor NYU, respeitando os preceitos de Allen (MEYER et al, 2003), porém as variações nos resultados, devido à melhora motora espontânea parcial (SOUZA et al, 2009) ou total (ŠEDÝ et al, 2009, após alguns dias de lesão medular, justifi cam mais estudos que visem a um modelo mais fi dedigno.…”

Section: Introductionunclassified

Padronização e avaliação histológica de um modelo experimental de lesão medular

et al. 2014

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“…1 Following acute SCI from blunt trauma, thrombin, a serine protease and key component of the coagulation cascade, rapidly increases from basal picomolar levels 2 at injury sites and elevated levels are sustained for several days due to expression by endothelial and astrocytic cells. 3, 4 Thrombin administered to healthy rat brains induces histological changes resembling inflammation and glial scarring, 5 indicating it may impede recovery of damaged neural networks.…”

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confidence: 99%

MMP9-sensitive polymers mediate environmentally-responsive bivalirudin release and thrombin inhibition

et al. 2015

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MMP9-responsive bivalirudin-HPMA copolymers were synthesized for direct, local administration in rat spinal cord contusion injury models. Polymer-conjugated bivalirudin peptides maintained activity while demonstrating enzyme-mediated release upon MMP9 exposure and prolonged release from hyaluronic acid/methylcellulose (HAMC) hydrogels compared to free bivalirudin peptide. Localized administration of bivalirudin copolymers in vivo at the site of rat spinal cord injury decreased cellular proliferation and astrogliosis, suggesting the bivalirudin copolymer and HAMC hydrogel system are a promising therapeutic intervention for reducing immediate inflammatory responses and long term scarring.

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Contusion Models of Spinal Cord Injury in Rats

Cited by 5 publications

References 31 publications

Evaluation of Epidermal Neural Crest Stem Cells in Organotypic Spinal Cord Slice Culture Platform

Evaluation of Epidermal Neural Crest Stem Cells in Organotypic Spinal Cord Slice Culture Platform

Padronização e avaliação histológica de um modelo experimental de lesão medular

MMP9-sensitive polymers mediate environmentally-responsive bivalirudin release and thrombin inhibition

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