Convergence of sphingolipid desaturation across over 500 million years of plant evolution

Resemann, Hanno Christoph; Herrfurth, Cornelia; Feussner, Kirstin; Hornung, Ellen; Ostendorf, Anna K.; Gömann, Jasmin; Mittag, Jennifer; Gessel, Nico van; Vries, Jan de; Ludwig‐Müller, Jutta; Markham, Jonathan E.; Reski, Ralf; Feußner, Ivo

doi:10.1038/s41477-020-00844-3

Cited by 42 publications

(53 citation statements)

References 75 publications

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“…Lu et al (2019) summarized the fatty acid compositions and contents in several moss and liverwort species from previous studies. Recently, it was reported that the lipidome of P. patens protonema comprising 733 molecular species derived from glycerolipids, sterol lipids and sphingolipids, whereas Arabidopsis plants harbor only about 54% of this diversity (Resemann et al, 2021). Moreover, a sphingolipid-modifying enzyme was identified that contributes to pathogen defence and cold tolerance, but has no homolog in seed plants (Resemann et al, 2021).…”

Section: Lipids and Lipid-derivativesmentioning

confidence: 99%

“…Recently, it was reported that the lipidome of P. patens protonema comprising 733 molecular species derived from glycerolipids, sterol lipids and sphingolipids, whereas Arabidopsis plants harbor only about 54% of this diversity (Resemann et al, 2021). Moreover, a sphingolipid-modifying enzyme was identified that contributes to pathogen defence and cold tolerance, but has no homolog in seed plants (Resemann et al, 2021). Large amounts of polyunsaturated C20 fatty acids in bryophytes imply that they can produce a broad range of oxylipins (Scholz et al, 2012).…”

Section: Lipids and Lipid-derivativesmentioning

confidence: 99%

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Natural Products from Bryophytes: From Basic Biology to Biotechnological Applications

Horn

Pascal

Lončarević

et al. 2021

Critical Reviews in Plant Sciences

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Natural products from plants have served mankind in a wide range of applications, such as medicines, perfumes, or flavoring agents. For this reason, synthesis, regulation and function of plant-derived chemicals, as well as the evolution of metabolic diversity, has attracted researchers all around the world. In particular, vascular plants have been subject to such analyses due to prevalent characteristics such as appearance, fragrance, and ecological settings. In contrast, bryophytes, constituting the second largest group of plants in terms of species number, have been mostly overlooked in this regard, potentially due to their seemingly tiny, simple and obscure nature. However, the identification of highly interesting chemicals from bryophytes with potential for biotechnological exploitation is changing this perception. Bryophytes offer a high degree of biochemical complexity, as a consequence of their ecological and genetic diversification, which enable them to prosper in various, often very harsh habitats. The number of bioactive compounds isolated from bryophytes is growing rapidly. The rapidly increasing wealth of bryophyte genetics opens doors to functional and comparative genomics approaches, including disentangling of the biosynthesis of potentially interesting chemicals, mining for novel gene families and tracing the evolutionary history of metabolic pathways. Throughout the last decades, the moss Physcomitrella (Physcomitrium patens) has moved from being a model plant together with Marchantia polymorpha in fundamental biology into an attractive host for the production of biotechnologically relevant compounds such as biopharmaceuticals. In the future, bryophytes like the moss P. patens might also be attractive candidates for the production of novel bryophytederived chemicals of commercial interest. This review provides a comprehensive overview of natural product research in bryophytes from different perspectives together with biotechnological advances throughout the last decade.

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Section: Lipids and Lipid-derivativesmentioning

confidence: 99%

Section: Lipids and Lipid-derivativesmentioning

confidence: 99%

Natural Products from Bryophytes: From Basic Biology to Biotechnological Applications

Horn

Pascal

Lončarević

et al. 2021

Critical Reviews in Plant Sciences

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“…In fact, subtle changes in sphingolipids can induce radical effects on the development of arabidopsis when exposed to abiotic stress such as cold. For example, it was very recently demonstrated that the VLCFAs content in sphingolipids, precisely that of the monounsaturated C24 and C26 fatty acids, was increased in response to cold, with the enzyme catalyzing the desaturation reaction, AtADS2, being upregulated [ 130 ]. Similarly, LCB desaturation, in particular at the Δ8 position of the LCB by sphingolipid Δ8 LCB desaturases, was shown to play a role in cold tolerance in tomato [ 131 ] and arabidopsis [ 132 ].…”

Section: Vlcfa and Sphingolipids In Plant Responses To Abiotic And Biotic Stressesmentioning

confidence: 99%

Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

Batsale

Bahammou

Fouillen

et al. 2021

Cells

118

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Very-long-chain fatty acids (i.e., fatty acids with more than 18 carbon atoms; VLCFA) are important molecules that play crucial physiological and structural roles in plants. VLCFA are specifically present in several membrane lipids and essential for membrane homeostasis. Their specific accumulation in the sphingolipids of the plasma membrane outer leaflet is of primordial importance for its correct functioning in intercellular communication. VLCFA are found in phospholipids, notably in phosphatidylserine and phosphatidylethanolamine, where they could play a role in membrane domain organization and interleaflet coupling. In epidermal cells, VLCFA are precursors of the cuticular waxes of the plant cuticle, which are of primary importance for many interactions of the plant with its surrounding environment. VLCFA are also major components of the root suberin barrier, which has been shown to be fundamental for nutrient homeostasis and plant adaptation to adverse conditions. Finally, some plants store VLCFA in the triacylglycerols of their seeds so that they later play a pivotal role in seed germination. In this review, taking advantage of the many studies conducted using Arabidopsis thaliana as a model, we present our current knowledge on the biosynthesis and regulation of VLCFA in plants, and on the various functions that VLCFA and their derivatives play in the interactions of plants with their abiotic and biotic environment.

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“…The GT construct pRKO25.2 (Hohe et al, 2004) contains a 1920 bp cDNA fragment of the cold-responsive gene Pp3c21_180V3, encoding sphingolipid fatty acid desaturase (PpSFD) (Beike et al 2015;Resemann et al, 2021). This construct contains the coding sequence for neomycin phosphotransferase (npt II) driven by a nopalinsynthase (NOS) promoter and terminator.…”

Section: Transformationmentioning

confidence: 99%

“…In order to investigate if the DNA-DSB repair is influenced by ploidy level, the rates of GT via the HR pathway were quantified in two haploid lines (Haploid A, Haploid B) and three diploid lines (Diploid A, Diploid B, Diploid C). For this purpose, the lines were transformed with a KO construct containing a 1920 bp cDNA fragment encoded by the cold-responsive gene Pp3c21_180V3, encoding sphingolipid fatty acid desaturase (PpSFD) (Beike et al, 2015;Hohe et al 2004;Resemann et al, 2021). The GT rate was defined as the number of KO plants divided by the total number of transformantsthe latter characterized by survival on selection medium and the presence of the selection marker (npt II; verified via PCR analysis).…”

Section: Gt Rate Is Reduced In Diploid Physcomitrella Cellsmentioning

confidence: 99%

Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

Rempfer

Wiedemann

Schween

et al. 2021

Preprint

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Qualitative changes in gene expression after an autopolyploidization event, a pure duplication of the whole genome, might be relevant for a different regulation of molecular mechanisms between angiosperms growing in a life cycle with a dominant diploid sporophytic stage and the haploid-dominant bryophytes. Whereas angiosperms repair DNA double strand breaks (DSB) preferentially via non-homologous end joining (NHEJ), in bryophytes homologous recombination (HR) is the main DNA-DSB repair pathway facilitating the precise integration of foreign DNA into the genome via gene targeting (GT). Here, we studied the influence of ploidy on gene expression patterns and GT efficiency in the moss Physcomitrella using haploid plants and autodiploid plants, generated via an artificial duplication of the whole genome. Single cells (protoplasts) were transfected with a GT construct and material from different time-points after transfection was analysed by microarrays and SuperSAGE sequencing. In the SuperSAGE data, we detected 3.7% of the Physcomitrella genes as differentially expressed in response to the whole genome duplication event. Among the differentially expressed genes involved in DNA-DSB repair was an upregulated gene encoding the X-ray repair cross-complementing protein 4 (XRCC4), a key player in NHEJ. Analysing the GT efficiency, we observed that autodiploid plants were significantly GT suppressed (p<0.001) attaining only one third of the expected GT rates. Hence, an alteration of global transcript patterns, including genes related to DNA repair, in autodiploid Physcomitrella plants correlated with a drastic suppression of HR.

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Convergence of sphingolipid desaturation across over 500 million years of plant evolution

Cited by 42 publications

References 75 publications

Natural Products from Bryophytes: From Basic Biology to Biotechnological Applications

Natural Products from Bryophytes: From Basic Biology to Biotechnological Applications

Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses

Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

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