Conversion of 18∶3 Δ9cis, 12cis, 15trans in rat liver microsomes

Chardigny, Jean‐Michel; Blond, Jean Paul; Brétillon, Lionel; Mager, Estelle; Poullain, Didier; Martine, Lucy; Vatèle, Jean‐Michel; Noël, Jean‐Pierre; Sébédio, Jean‐Louis

doi:10.1007/s11745-997-0093-1

Cited by 10 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding physiological effects of a -LAGI [81] c9,c12,t15 is considered to be a weak inhibitor of the D 6-desaturation of C18 : 3n-3 [84]. Further, a -LAGI were reported to alter the biosynthesis of arachidonic acid at the D 5-desaturation step [85].…”

Section: Resultsmentioning

confidence: 99%

C18 : 1, C18 : 2 and C18 : 3trans andcis fatty acid isomers including conjugatedcisδ9,transδ11 linoleic acid (CLA) as well as total fat composition of German human milk lipids

Precht

Molkentin

1999

Nahrung

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

C18 : 1, C18 : 2 and C18 : 3trans andcis fatty acid isomers including conjugatedcisδ9,transδ11 linoleic acid (CLA) as well as total fat composition of German human milk lipids

Precht

Molkentin

1999

Nahrung

View full text Add to dashboard Cite

“…The animals were anesthetized with diethyl ether at 8:30 A.M. in order to avoid variation in enzyme activities due to circadian rhythm (25), exsanguinated, and the livers were quickly excised. Microsomes were prepared using a slight modification of the previously described method (26). Briefly, each liver (12.5 ± 0.33 g, mean ± SEM) was homogenized in sucrose and phosphate buffer (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Effects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro

Brétillon

Chardigny

Grégoire

et al. 1999

Lipids

141

View full text Add to dashboard Cite

The influence of individual conjugated linoleic acid (CLA) isomers on the delta6 desaturation of linoleic and alpha-linolenic acids and on the delta9 desaturation of stearic acid was investigated in vitro, using rat liver microsomes. The delta6 desaturation of 18:2n-6 was decreased from 23 to 38% when the ratio of 9cis,11trans-18:2 to 18:2n-6 increased from 0.5 to 2. The compound 10trans,12cis-18:2 exhibited a similar effect only at the highest concentration. The delta6 desaturation of alpha-linolenic acid was slightly affected by the presence of CLA isomers. The sole isomer to induce an inhibitory effect on the delta9 desaturation of stearic acid was 10trans,12cis-18:2.

show abstract

“…More recently, we have also shown that trans isomers of a-linolenic acid (9trans and 15trans) also inhibit the D6 desaturation of a-linolenic acid itself [47,48].…”

Section: Trans Fa and Bioconversion Of Essential Famentioning

confidence: 99%

Metabolism of trans and conjugated fatty acids

Chardigny

Clouet

Combe

et al. 2007

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

Metabolism of trans and conjugated fatty acidsTrans fatty acids (FA) enter all the metabolic pathways of bioconversion, oxidation and lipid storage and are incorporated into all tissues. They also interfere with the polyunsaturated FA metabolic pathways. Among trans FA, major attention is paid to elaidic and vaccenic acids. The latter is bioconverted into rumenic acid, a conjugated linoleic acid (CLA) isomer, through the D9 desaturase pathway. Considering CLA isomers, they are also bioconverted by desaturases and the elongation pathway, but the significance of this conversion is not fully understood. This review considers these different aspects, with particular attention to human data. Incorporation and bioconversion as well as oxidative metabolism of trans FA (monoenes, polyenes and conjugated fatty acids) are rapidly overviewed. Furthermore, their impact on the metabolism of other FA is also considered. Keywords Incorporation of trans FA into tissues 2.1 Trans 18:1 isomersOhlrogge et al.[1] extensively studied the occurrence of trans 18:1 FA in tissues after death of nine subjects. These trans FA were reported in all the lipid classes from liver, heart, red blood cells and plasma. Later, Rocquelin et al.[2] reported that human cardiac biopsies contained 0.7-0.8% of total FA as trans 18:1. Elaidic acid was suggested as being the major one in phosphatidylcholine, but the relevance has to be confirmed, considering the GC method available.Generally, analyses are performed on tissues obtained from less invasive experiments (blood samples, adipose tissue, milk). For example, data from the EURAMIC study, which was carried out in the 1990s, showed that trans 18:1 isomers in adipose tissue ranged from 0.4 to 2.4% of total FA [3,4]. These values are similar to those reported by Boue et al. [5] in adipose tissue of French women, which contained about 2% of trans 18:1 isomers. Generally, it is demonstrated that the trans FA content in adipose tissue is related to the dietary intake [6]. However, a recent study failed to demonstrate such a correlation [7]. In human milk, trans 18:1 isomers have been reported for several years. In the early report of Picciano and Perkins [8], trans 18:1 was reported to represent 2-4% of total FA. More recently, it has been reported that samples from US women contain 5.1 6 2.0% 18:1 trans isomers (range 1.5-11.6%), with D10t being the most abundant isomer [9]. In French samples, the mean content in 1995 was lower (1.9% of total FA), with vaccenic acid being the major isomer [10]. Such differences illustrate that the trans FA sources are different according to the countries. Trans polyunsaturated FA Review ArticleEur. J. Lipid Sci. Technol. 109 (2007) 930-934 TFAs and CLA metabolism 931 of n-3 trans isomers is lower than that of trans 18:1, as the intake is lower (,200 mg/day).

show abstract

Conversion of 18∶3 Δ9cis, 12cis, 15trans in rat liver microsomes

Cited by 10 publications

References 17 publications

C18 : 1, C18 : 2 and C18 : 3trans andcis fatty acid isomers including conjugatedcisδ9,transδ11 linoleic acid (CLA) as well as total fat composition of German human milk lipids

C18 : 1, C18 : 2 and C18 : 3trans andcis fatty acid isomers including conjugatedcisδ9,transδ11 linoleic acid (CLA) as well as total fat composition of German human milk lipids

Effects of conjugated linoleic acid isomers on the hepatic microsomal desaturation activities in vitro

Metabolism of trans and conjugated fatty acids

Contact Info

Product

Resources

About