Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Hassanein, A. M.; Ibraheim, Ibrahim A.; Galal, Abel-Naser; Salem, Jehan

doi:10.4236/ajps.2011.23055

Cited by 11 publications

(20 citation statements)

References 25 publications

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“…In this study, the diameters of the synthetic seed with a single and double alginate coating were about 5-6 mm and 7-8 mm, respectively (Figure 1). These were similar to those reported in other studies [12,[17][18][19][20]. The diameter of the synthetic Persian violet fruit (10-11 mm) was higher than the synthetic seed.…”

Section: Alginate Encapsulationsupporting

confidence: 91%

“…Interestingly, there was no significant difference in the post-storage germination rates of the microshoots encapsulated individually in a single layer and the synthetic fruit. However, the apple and banana synthetic seeds encapsulated in a single alginate coating did not exhibit substantially different germination percentages than synthetic seeds with double alginate coating [11,12,17]. The effect of double coating on the storability of these synthetic seeds was also not the focus of these studies.…”

Section: Germination and Viability Assessmentmentioning

confidence: 92%

“…If required, the encapsulated propagules or commonly called "synthetic seeds," could possibly be used to facilitate short-term storage required for germplasm exchange or delayed sowing [5,6]. In most studies on synthetic seeds, a plant propagule is often enclosed within a single alginate coating [7,8], while variations of this approach, including double coating layers, have also been investigated [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Phanomchai

Bodhipadma

Noichinda

et al. 2022

Plants

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Microshoots have been widely used for micropropagation. It may be necessary to store microshoots for a short period of time, for example in germplasm exchange needing transport to other research groups. Here, we investigated the short-term storability of alginate-encapsulated Persian violet (Exacum affine Balf. f. ex Regel) microshoots at 4 °C and 25 °C. After storage, the encapsulated microshoots were sown on basal Murashige and Skoog medium for germination and viability determination using tetrazolium chloride staining. The results showed that one or five microshoots encapsulated with a single alginate layer could be stored at 4 °C for up to 30 days, while the percentages of germination and viability of the microshoots encapsulated with two layers of alginate were greatly reduced upon storage. This is the first report on the storability of alginate-encapsulated multiple microshoots, which could be a more efficient way to encapsulate microshoots used for short-term cold storage.

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Section: Alginate Encapsulationsupporting

confidence: 91%

Section: Germination and Viability Assessmentmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Phanomchai

Bodhipadma

Noichinda

et al. 2022

Plants

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“…The synthetic seed technology which involves the encapsulation of small sized plant propagules with protective nutrient gel provides another promising option for mass propagation because of higher scale up capacity, possibility of automation of whole product process and direct delivery to field. Though synthetic seeds have been developed for potato (Patel et al, 2000), apple (Piccioni, 1997), Catharanthus roseus (Maqsood et al, 2012), banana (Hassanein et al, 2011), eggplant (Huda et al, 2007), black pepper (Nair and Gupta, 2007) and orchid (Mohanraj et al, 2009;Nagananda et al, 2011), reports on synthetic seed production in tomato are very scarce. The present study was conducted with an aim of establishing efficient and reproducible in vitro regeneration protocol for large scale propagation of tomato through seeds and shoot-tip culture.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Oceania¹,

Doni²,

Tikendra³

et al. 2014

J. of Plant Sciences

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Tomato is a highly popular crop for its rich nutritional values and constitutes an integral part of daily diets for substantial portion of human population of the world. Because of limitations in conventional breeding methods and increasing demand of this important crop for fast growing population, large scale propagation of tomato through plant tissue culture techniques becomes highly significant. Efficient in vitro generation protocols for tomato have been established successfully by employing seed and tissue culture derived shoot-tip explants. 6-benzyl amino purine (BAP) was highly effective in inducing seed germination and rapid leaf multiplication though presence of 0.4% activated charcocal (AC) had negative influence on culture development. The used of in vitro derived shoot-tips as explants removed the deleterious effect of AC resulting in the production of highest number of leaves in MS+3 mg LG NAA generated highest root formation when shoot-tip propagules were used for culture initiation. Preliminary investigation of synthetic seed development of tomato revealed the gel matrix produced from the complexation between 2% sodium alginate and 100 mM CaCl 2 for 20-40 min as the most favourable for efficient encapsulation of in vitro derived shoot-tip propagules. The in vitro protocols developed from the present investigation can be utilized for propagation of tomato in larger scale for commercial purposes as well as local domestic consumption.

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“…pode diminuir os custos de produção pela eliminação das etapas de enraizamento e aclimatação; além da possibilidade do estabelecimento dos propágulos diretamente a campo num estágio mais precoce do que em sistemas convencionais de propagação (SAIPRASAD, 2001). Essa técnica foi utilizada com sucesso por Sandoval-Yugar et al (2009), na cultivar 'Grande Naine', e Hassanein et al (2011), na cultivar Hindi, obtendo altas taxas de conversão, 75% e 98.34%, respectivamente. Apesar dos resultados obtidos com esta tecnologia, há necessidade de inovações e melhorias quanto à adequação dos tipos de concentrações dos constituintes a serem introduzidos à matriz de encapsulamento.…”

Section: Influence Of the Composition Of Encapsulation Matrix Of Micrunclassified

Influência da composição da matriz de encapsulamento de microbrotos de banana (Musa sp.) cv. Prata-Anã clone Gorutuba

et al. 2014

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RESUMO -A banana é a principal fruta no comércio internacional e a mais popular no mundo. A produção de sementes sintéticas vem destacando-se como uma importante técnica para a micropropagação e conservação in vitro de várias espécies. O objetivo deste estudo foi avaliar a influência da composição da matriz de encapsulamento na conversão de microbrotos de banana cv. Prata-Anã clone Gorutuba. Os microbrotos de banana cv. Prata-Anã clone Gorutuba foram encapsulados, empregando-se na matriz alginato de sódio: água destilada e MS (50% e 100%), acrescidos ou não com carvão ativado (1,5 g L -1 ) em duas épocas de avaliação (15 e 30 dias). Foram testados três níveis de BAP (0; 3; 6 mg L -1 ) adicionados ou não a 2,5 mg L -1de ANA, em duas épocas de avaliação (15 e 30 dias). Os dois experimentos foram inteiramente casualizados, sendo um fatorial 3 x 2 x 2, com seis repetições e cinco unidades encapsuláveis por parcela. Aos quinze dias, avaliou-se a conversão, e aos 30 dias, avaliou-se conversão, altura e enraizamento das plantas. A constituição da cápsula quando se utilizaram diferentes concentrações de meio MS, com ou sem a presença de carvão ativado, não influenciou na taxa de conversão e na altura das plantas. As cápsulas sem adição de reguladores de crescimento resultaram em melhor conversão dos microbrotos; entretanto, a concentração de 3 mg L -1 de BAP+2,5 mg L -1 de ANA proporcionaram maior altura de plantas. A maior taxa de enraizamento ocorre nas cápsulas contendo MS 100% e carvão ativado, e nas cápsulas com 0 e 3 mg L -1 de BAP, independentemente da adição de ANA. Aos 30 dias, é obtida maior conversão das sementes sintéticas. Termos para indexação: Semente sintética, Musa sp., fitorreguladores,carvão ativado. INFLUENCE OF THE COMPOSITION OF ENCAPSULATION MATRIX OF MICROSHOOTS OF BANANA (Musa sp.) CV. PRATA-ANÃ GORUTUBA CLONEABSTRACT -Banana is the main fruit in the international trade and the most popular one in the world. The production of synthetic seeds comes highlighting itself as an important technique for the micropropagation and in vitro conservation of several species. The aim of this study was to evaluate the influence of the composition of the encapsulation matrix in the conversion of microshoots of banana cv. Prata Anã Gorutuba clone. The microshoots of banana cv. Prata-Anã Gorutuba clone were encapsulated being used in the sodium alginate matrix: water and MS (50 % and 100 %), added or not with active charcoal (1.5 g L -1 ) in two evaluation periods (15 and 30 days). Three levels of BAP (0, 3, 6 mg L -1 ) added or not to 2.5 mg L -1 of NAA were tested in two evaluation periods (15 and 30 days). The two experiments were entirely at random, being a factorial 3 x 2 x 2, with six repetitions and five encapsulated units per plot. On the fifteenth day evaluated the conversion, on the 30th day evaluated the conversion, the height and the rooting of the plants. The capsule constitution, when different MS medium concentrations were used, with or without the presence of active charcoal, did not influence the...

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Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Abstract: ABSTRACT

Cited by 11 publications

References 25 publications

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Influência da composição da matriz de encapsulamento de microbrotos de banana (Musa sp.) cv. Prata-Anã clone Gorutuba

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