Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Vastano, Marco; Corrado, Iolanda; Sannia, Giovanni; Solaiman, Daniel K. Y.; Pezzella, Cinzia

doi:10.1038/s41598-019-50278-x

Cited by 42 publications

(16 citation statements)

References 26 publications

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“…Escherichia coli has been used as chassis for the production of PHBHHx by introducing synthase genes specific for mcl-precursors and/or acting on the pathways channeling specific precursors (Taguchi et al, 1999 ; Lu et al, 2003 ). A high mol% HHx (50 mol%) has been achieved in E. coli strain engineered with the PHA biosynthetic operon from Bacillus cereus , using fatty acids as well as complex C-sources such as waste frying oils as substrates (Vastano et al, 2017 , 2019 ).…”

Section: In Vivo Strategies To Modulate Phb Properties: Syntmentioning

confidence: 99%

“…Previously, mcl-PHA production has been achieved by supplying even-chain n-alkanoic acids from C8 to C22 (Ballistreri et al, 2001 ). The use of complex oily sources, including waste frying oils, has been also explored for the synthesis of mcl-PHA from different Pseudomonas strains (Haba et al, 2007 ; Gamal et al, 2013 ; Follonier et al, 2014 ; Vastano et al, 2019 ).…”

Section: In Vivo Strategies To Modulate Phb Properties: Syntmentioning

confidence: 99%

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In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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The transition toward “green” alternatives to petroleum-based plastics is driven by the need for “drop-in” replacement materials able to combine characteristics of existing plastics with biodegradability and renewability features. Promising alternatives are the polyhydroxyalkanoates (PHAs), microbial biodegradable polyesters produced by a wide range of microorganisms as carbon, energy, and redox storage material, displaying properties very close to fossil-fuel-derived polyolefins. Among PHAs, polyhydroxybutyrate (PHB) is by far the most well-studied polymer. PHB is a thermoplastic polyester, with very narrow processability window, due to very low resistance to thermal degradation. Since the melting temperature of PHB is around 170–180°C, the processing temperature should be at least 180–190°C. The thermal degradation of PHB at these temperatures proceeds very quickly, causing a rapid decrease in its molecular weight. Moreover, due to its high crystallinity, PHB is stiff and brittle resulting in very poor mechanical properties with low extension at break, which limits its range of application. A further limit to the effective exploitation of these polymers is related to their production costs, which is mostly affected by the costs of the starting feedstocks. Since the first identification of PHB, researchers have faced these issues, and several strategies to improve the processability and reduce brittleness of this polymer have been developed. These approaches range from the in vivo synthesis of PHA copolymers, to the enhancement of post-synthesis PHB-based material performances, thus the addition of additives and plasticizers, acting on the crystallization process as well as on polymer glass transition temperature. In addition, reactive polymer blending with other bio-based polymers represents a versatile approach to modulate polymer properties while preserving its biodegradability. This review examines the state of the art of PHA processing, shedding light on the green and cost-effective tailored strategies aimed at modulating and optimizing polymer performances. Pioneering examples in this field will be examined, and prospects and challenges for their exploitation will be presented. Furthermore, since the establishment of a PHA-based industry passes through the designing of cost-competitive production processes, this review will inspect reported examples assessing this economic aspect, examining the most recent progresses toward process sustainability.

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Section: In Vivo Strategies To Modulate Phb Properties: Syntmentioning

confidence: 99%

Section: In Vivo Strategies To Modulate Phb Properties: Syntmentioning

confidence: 99%

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Turco

Santagata

Corrado

et al. 2021

Front. Bioeng. Biotechnol.

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“…As mentioned above, we have developed a microbial platform for the production of MCDCAs from palmitic acid and stearic acid as direct feedstocks. To lower the costs of microbial cell factories, the use of cheaper feedstocks, including lignocellulosic biomass, waste oil, and crude glycerol, has been proposed. − ,− Here, we investigated the potential of using waste feedstock for sustainable MCDCAs production.…”

Section: Resultsmentioning

confidence: 99%

“…The toxic chemicals contained in WCO can cause organic or inorganic pollution of water bodies, negatively affecting water quality and aquatic organisms . Moreover, an elevated WCO content can coat the activated sludge, making it difficult to degrade waste through oxygen transfer . Therefore, recycling and reutilization of food waste facilitate circular economy.…”

mentioning

confidence: 99%

Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil

Cheng

Zhao

et al. 2021

ACS Synth. Biol.

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The recycle and reutilization of food wastes is a promising alternative for supporting and facilitating circular economy. However, engineering industrially relevant model organisms to use food wastes as their sole carbon source has remained an outstanding challenge so far. Here, we reprogrammed Escherichia coli metabolism using modular pathway engineering followed by laboratory adaptive evolution to establish a strain that can efficiently utilize waste cooking oil (WCO) as the sole carbon source to produce monomers of bioplastics, namely, medium-chain α,ω-dicarboxylic acids (MCDCAs). First, the biosynthetic pathway of MCDCAs was designed and rewired by modifying the βoxidation pathway and introducing an ω-oxidation pathway. Then, metabolic engineering and laboratory adaptive evolution were applied for improving the pathway efficiency of fatty acids utilization. Finally, the engineered strain E. coli AA0306 was able to produce 15.26 g/L MCDCAs with WCO as the sole carbon source. This study provides an economically attractive strategy for biomanufacturing bioplastics from food wastes, which has a great potentiality to be developed as a wide range of enabling biotechnologies for achieving green revolution.

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“…To decrease the production costs, a PHA producing strain should be able to grow to high cell densities and accumulate large amounts of PHA at the account of inexpensive carbon resources such as glycerol (Gahlawat & Soni, 2017;Poblete-Castro et al, 2014), waste cooking oil (Sangkharak et al, 2020;Vastano et al, 2019), or other low-cost biomass (whey, starch, spent coffee grounds, wastewaters, wheat, and rice straw, lignin, etc. ; Alcântara et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate

et al. 2021

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Conversion of no/low value waste frying oils into biodiesel and polyhydroxyalkanoates

Cited by 42 publications

References 26 publications

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

In vivo and Post-synthesis Strategies to Enhance the Properties of PHB-Based Materials: A Review

Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil

Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate

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