Conversion of ortho-para H2O isomers in water and a jump in erythrocyte fluidity through a microcapillary at a temperature of 36.6±0.3°C

Першин, С. М.

doi:10.3103/s1541308x09040025

Cited by 33 publications

(41 citation statements)

References 32 publications

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“…In the paper by Pershin et al [33], it is noted that under the action of a pipette (and in our case, under the action of the peristaltic pump), a change in the hydration surface layer can be observed. In [33], it is stated that there is a hydration shell near the surface of proteins, which represents an ice-like structure. At this point, it is known that the ortho/para ratio of 3:1, which is valid for gases at room temperature, shifts in water towards a more than twofold increase in the amount of para-H 2 O isomers.…”

Section: Discussionsupporting

confidence: 48%

“…In our previous studies, we demonstrated that under certain conditions, upon the pumping of water through an injector with a peristaltic pump in the drop-by-drop mode at 0.9 mL/min flowrate and a temperature of 38 • C, an accumulation of charge in the measuring cell has a linear-stepwise character [30]. The temperature of 38 • C is interesting, owing to the fact that it is near the viscosity-associated characteristic temperature point of water [33,34]. In the present work, the research on charge accumulation in the flow-based system for atomic force microscopy (AFM)-based fishing has been continued, and the influence of flow rate has been studied in a wide range from 0.9 to 7.2 mL/min has been studied.…”

Section: Discussionmentioning

confidence: 90%

“…This can be particularly sharply pronounced within the peristatic pump, where the pump rollers significantly squeeze the feeding pipe, thereby significantly distorting the fluid flow near the boundary layer. Another cause of the charge jumps is connected with the ortho-para conversion of water, and leads to a shift in the equilibrium in the boundary layer under the action of the peristaltic pump-this can be explained in a way analogous to [33]. In the paper by Pershin et al [33], it is noted that under the action of a pipette (and in our case, under the action of the peristaltic pump), a change in the hydration surface layer can be observed.…”

Section: Discussionmentioning

confidence: 99%

“…Another cause of the charge jumps is connected with the ortho-para conversion of water, and leads to a shift in the equilibrium in the boundary layer under the action of the peristaltic pump-this can be explained in a way analogous to [33]. In the paper by Pershin et al [33], it is noted that under the action of a pipette (and in our case, under the action of the peristaltic pump), a change in the hydration surface layer can be observed. In [33], it is stated that there is a hydration shell near the surface of proteins, which represents an ice-like structure.…”

Section: Discussionmentioning

confidence: 99%

“…Ortho-para conversion in water, associated with the anomalous properties of water (which has a complex heterostructure), is described in [33,34]. Since the studied range of supply flow rates is typical not only for systems for AFM-based fishing, but also for other proteomic detection systems (chromatographic, chromato-mass-spectrometric, nanowire-based ones, etc.…”

Section: Discussionmentioning

confidence: 99%

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The Dependence of Spontaneous Charge Generation in Water on its Flow Rate in a Flow-Based Analytical System

et al. 2020

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Highly sensitive biosensor systems are particularly sensitive to the charge state of an analyte. This charge state can have either a positive (for instance, in case of increasing the efficiency of fishing of low-abundant proteins) or negative effect (for instance, in case of the appearance of charge jumps upon the injection of analyte solution into a measuring cell, what can cause undesirable parasitic signals). Previously, it was demonstrated that upon the pumping of analyte solution through polymeric communications of biosensors with a peristaltic pump at a low (~1 mL/min) flow rate, an accumulation of charge, transferred by the liquid drops from the feeding system into the measuring cell, is observed. At this point, the time dependence of charge accumulation has a linear-stepwise form. In the present study, the influence of the flow rate of water on the parameters of the time dependence of the accumulation of charge in such a system—including the influence on the stepwise charge accumulation—has been investigated. The measurements have been performed with a highly sensitive electrometer sensor at 38 °C, which corresponds to a pathological state of a human body. It has been found that a linear-stepwise time dependence of charge accumulation is observed in a wide range of water flow rates (V= 0.9 to 7.2 mL/min). At that point, upon increasing the flow rate with the transition from the drop-by-drop mode of water supply (0.9 mL/min) to the jet flow (7.2 mL/min), an increase in the absolute value of accumulated charge is observed, but the magnitude of the charge jumps does not change significantly. Thus, the amount of charge accumulated in the cell ambiguously depends on the water flow rate—i.e., this dependence can be non-linear. Accounting for the discovered phenomenon is important in the development of new, more accurate models describing physicochemical properties of aqueous solutions and hemodynamics. This effect should also be taken into account in the development of highly sensitive diagnostic systems intended for the detection of single biomarkers of pathologies in humans and crops, as well as in other living systems. In low-concentration systems, the occurrence of a charge can become a significant factor affecting the efficiency of detection of biomolecules and the reliability of the data obtained. The detection of biomolecules present in the solution at low concentrations is in high demand in medical diagnostics for the revelation of biomarkers at the early asymptomatic stage of various diseases, including aggressive forms of cancer.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 90%