Convex vs concave surface nano-curvature of Ta2O5 thin films for tailoring the osteoblast adhesion

Vaidulych, Mykhailo; Pleskunov, Pavel; Kratochvíl, Jiří; Mašková, Hana; Kočová, Pavlína; Nikitin, Daniil; Hanuš, Jan; Kylián, Ondřej; Štěrba, Ján; Biederman, Hynek; Choukourov, Andrei

doi:10.1016/j.surfcoat.2020.125805

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…Another important characteristic of the c-TIRF platform is biocompatibility. HeLa cells have previously been seen to grow on Ta 2 O 5 waveguides 17 , and a study of osteoblasts on various topologies of Ta 2 O 5 showed that there was no decrease in cell viability compared to glass or plastic cell culture plates, although there were some differences in cell adhesion 39,40 . HeLa cells could be easily grown on our transparent waveguide chips, and showed minimal cell death after 1.5 days.…”

Section: Resultsmentioning

confidence: 99%

A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy

et al. 2021

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Total internal reflection fluorescence (TIRF) microscopy is an imaging technique that, in comparison to confocal microscopy, does not require a trade-off between resolution, speed, and photodamage. Here, we introduce a waveguide platform for chip-based TIRF imaging based on a transparent substrate, which is fully compatible with sample handling and imaging procedures commonly used with a standard #1.5 glass coverslip. The platform is fabricated using standard complementary metal-oxide-semiconductor techniques which can easily be scaled up for mass production. We demonstrate its performance on synthetic and biological samples using both upright and inverted microscopes, and show how it can be extended to super-resolution applications, achieving a resolution of 116 nm using super resolution radial fluctuations. These transparent chips retain the scalable field of view of opaque chip-based TIRF and the high axial resolution of TIRF, and have the versatility to be used with many different objective lenses, microscopy methods, and handling techniques. We see this as a technology primed for widespread adoption, increasing both TIRF’s accessibility to users and the range of applications that can benefit from it.

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Section: Resultsmentioning

confidence: 99%

A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy

et al. 2021

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“…Kita considered L to be ~100nm [14] and Schmid's measured value is 180nm, with which a good experimental and analytical agreement is achieved [25]. For deposited Ta 2 O 5 thin film, the measured correlation length are ranging from ~100nm [37], 400nm [38] and 49 to 282nm [39]. Due to the rather wide range of the correlation length reported, the relationship between scattering loss and correlation length is first investigated by calculating the loss of fundamental modes as a function of correlation length and core thickness using Eqns.…”

Section: Modal Field Coupling Efficiency and Scattering Loss Calculat...mentioning

confidence: 93%

Effect of Scattering Loss on Optimization of Waveguide Enhanced Raman Spectroscopy

Liu,

Ettabib,

Wilkinson

et al. 2024

J. Lightwave Technol.

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Waveguide enhanced Raman spectroscopy (WERS) is a promising sensing technology requiring accurate waveguide optimization to increase the pump/signal surface intensity. Traditionally, WERS has been focused on single-mode operation, which results in stringent waveguide parameter control and increased propagation losses. In this work, we have studied theoretically and experimentally the impact of planar waveguide thickness on surface scattering losses and the waveguide propagation losses. In our study, we consider radiation from a Raman-emitting dipole on a waveguide can be captured back into the waveguide in polarization and spatial modes different from the pump mode, provided the waveguide can support them. In the case of randomly-distributed dipoles, we consider the Raman gain coefficients corresponding to all possible combinations of pump and signal polarization and spatial modes. We have introduced a new generalized FOM to optimize planar waveguidebased WERS sensors under multimode excitation/collection operation and waveguidethickness-and mode-dependent propagation losses. The FOM is shown to increase with the mode order as a result of the combination of substantial reduction in propagation loss, increased number of collecting modes, longer optimum sensing lengths, and this occurs despite the concomitant surface intensity reduction. This implies that in the case of randomly distributed dipoles, the single-mode pump excitation is not strictly required, and multimode pump excitation will give superior conversion efficiencies.

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“…Kita considered L to be ~100nm [14] and Schmid's measured value is 180nm, with which a good experimental and analytical agreement is achieved [25]. For deposited Ta2O5 thin film, the measured correlation length are ranging from ~100nm [37], 400nm [38] and 49 to 282nm [39]. Due to the rather wide range of the correlation length reported, the relationship between scattering loss and correlation length is first investigated by calculating the loss of fundamental modes as a function of correlation length and core thickness using Eqns.…”

Section: Modal Field Coupling Efficiency and Scattering Loss Calculat...mentioning

confidence: 94%

Effect of scattering loss on optimization of waveguide enhanced Raman spectroscopy

Liu¹,

Ettabib²,

Wilkinson³

et al. 2023

Preprint

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Waveguide enhanced Raman spectroscopy (WERS) is a promising sensing technology requiring accurate waveguide optimization to increase the pump/signal surface intensity. Traditionally, WERS has been focused on single-mode operation, which results in stringent waveguide parameter control and increased propagation losses. In this work, we have studied theoretically and experimentally the impact of planar waveguide thickness on surface scattering losses and the waveguide propagation losses. In our study, we consider radiation from a Raman-emitting dipole on a waveguide can be captured back into the waveguide in polarization and spatial modes different from the pump mode, provided the waveguide can support them. In the case of randomly-distributed dipoles, we consider the Raman gain coefficients corresponding to all possible combinations of pump and signal polarization and spatial modes. We have introduced a new generalized FOM to optimize planar waveguide-based WERS sensors under multimode excitation/collection operation and waveguide-thickness- and mode-dependent propagation losses. The FOM is shown to increase with the mode order as a result of the combination of substantial reduction in propagation loss, increased number of collecting modes, longer optimum sensing lengths, and this occurs despite the concomitant surface intensity reduction. This implies that in the case of randomly distributed dipoles, the single-mode pump excitation is not strictly required, and multimode pump excitation will give superior conversion efficiencies.

show abstract

Convex vs concave surface nano-curvature of Ta2O5 thin films for tailoring the osteoblast adhesion

Cited by 8 publications

References 54 publications

A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy

A transparent waveguide chip for versatile total internal reflection fluorescence-based microscopy and nanoscopy

Effect of Scattering Loss on Optimization of Waveguide Enhanced Raman Spectroscopy

Effect of scattering loss on optimization of waveguide enhanced Raman spectroscopy

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