CoO Nanozymes with Multiple Catalytic Activities Regulate Atopic Dermatitis

Abstract

Herein, we prepared CoO nanozymes with three types of enzyme catalytic activities for the first time, which have SOD-like, CAT-like, and POD-like catalytic activities. This is the first study to report the preparation of CoO nanoparticles with three types of enzyme catalytic activities by the one-pot method. By modifying the surface of CoO nanozymes with a carboxyl group, its biocompatibility enhanced, so it can be used in the field of life sciences. In vitro cytotoxicity and anti-H2O2-induced ROS experiments proved that CoO nanozymes can protect HaCaT cells against ROS and cytotoxicity induced by H2O2. In addition, an atopic dermatitis (AD) mouse model was established by topical application of MC903, which verified the anti-inflammatory effect of CoO nanozymes on the AD mouse model. Traditional drugs for the treatment of AD, such as dexamethasone, have significant side-effects. The side-effects include skin burns, telangiectasias, and even serious drug dependence. CoO nano-enzymes have a low cytotoxicity and its multiple enzyme-like catalytic activities can effectively protect cells and tissues in ROS environments, which proves that CoO nano-enzymes have high application potential in the field of anti-inflammation.

Intrinsic Multienzyme-like Activities of the Nanoparticles of Mn and Fe Cyano-Bridged Assemblies

“…The search for efficient nanozymes that functionally mimic the major antioxidant enzymes-catalase, superoxide dismutase, and peroxidase represents a possible approach to control and use reactions mediated by reactive oxygen species (ROS). Since researchers had established a peroxidase mimetic Fe 2 O 3 nanozyme in optical assay [1,2], investigations of transition metal oxides [3][4][5][6][7][8][9][10][11], macroheterocycles [12][13][14][15][16][17][18], and metal organic frameworks [19] have led to the discovery of further antioxidant (multi)enzyme mimetic compounds and electrocatalysts in chemical and electrochemical reactions mediated by ROS. One of these antioxidant enzyme mimetic compounds is Prussian blue (PB), which is referred to as an "artificial peroxidase" [20,21].…”

Green synthesis of M-type manganese-substituted strontium hexaferrite SrMnxFe12−xO19 nanoparticles with intrinsic antibacterial activity against human pathogenic bacteria

“…33,35,36 Similarly, they were used for water purification, 26 cancer hyperthermia treatment, 37 cytotoxic chemotherapy, 21 drug delivery, 21,38 and detecting biomolecules with catalase peroxidase enzyme mimetic activity. 39,40…”

“…ROS can be produced by SFs and HFs either directly or indirectly through a variety of mechanisms. 4,40,41 The presence of ROS significantly influences cell toxicity by causing cell rupture, nucleic acid damage, 42 and cytoplasmic leakage. 43 The absorption and uptake of HFs and SFs by cellular microorganisms play a critical role in cell damage, particularly when an excessive level of ROS are present.…”

Recent theranostic applications of hydrogen peroxide-responsive nanomaterials for multiple diseases

“…82 In addition to Mn, Co-based metal oxides have been proven that possessed SOD-like, CAT-like, and POD-like catalytic activities. 83,84 Based on those, cobaltosic oxide (Co 3 O 4 ) nanoparticles have been designed as biosensing platforms to detect H 2 O 2 . 85 In another study, Ce-based metal oxides have been reported that the ratio of Ce 3+ /Ce 4+ determined the enzyme-like properties, such as CAT mimic.…”