Cooking and drying as effective mechanisms in limiting the zoonotic effect of Mycobacterium bovis in beef

Merwe, M. Van Der; Bekker, Johan Leon; Merwe, Paul van der; Michel, Anita Luise

doi:10.4102/jsava.v80i3.189

Cited by 15 publications

(16 citation statements)

References 15 publications

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“…However, in the bovine/bubaline samples of the present study, 37% of the CITT+ animals exhibited NVL, of which 14.9% were positive both in the culture and in the nested-PCR. This raises concerns that zoonotic transmission, such as that of M. bovis , can survive the cooking process [43]. For this reason, sanitary policies involving PCR testing of CITT+/NVL animals should be considered.…”

Section: Discussionmentioning

confidence: 99%

Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

et al. 2014

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In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

et al. 2014

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“…However, in the bovine/bubaline samples of the present study, 40.7% of the CITT+ animals exhibited NVL, from which 22.5% were positive for MTC both in the culture and the nested-PCR. This raises concerns that zoonotic transmission, such as that of M. bovis , the main MTC species found in cattle, can survive the cooking process (Van der Merwe et al , 2009). For this reason, sanitary policies involving PCR testing of CITT+/NVL animals should be considered.…”

Section: Discussionmentioning

confidence: 99%

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

Araújo¹,

Osório²,

Jorge³

et al. 2014

Braz. J. Microbiol.

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Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.

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“…Therefore, it is imperative that cattle owners, traders, butchers and policy makers are made aware of the risks posed by meat-borne zoonoses that are prevalent in their areas. The information provided should also explain how zoonoses are transmitted in order to enable those at risk to make informed decisions as to how they might best protect themselves [8,9] . Frequently detected and reported abattoir diseases or conditions include fascioliasis, cysticercus of Taenia saginata (Cysticercus bovis), tuberculosis and hydatidosis [10,11] .…”

Section: Introductionmentioning

confidence: 99%

A survey of zoonotic diseases in trade cattle slaughtered at Tanga city abattoir: a cause of public health concern

Swai¹,

Schoonman

2012

Asian Pacific Journal of Tropical Biomedicine

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Cooking and drying as effective mechanisms in limiting the zoonotic effect of Mycobacterium bovis in beef

Cited by 15 publications

References 15 publications

Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1

Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR

A survey of zoonotic diseases in trade cattle slaughtered at Tanga city abattoir: a cause of public health concern

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