Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need

Zohny, Mona H.; Cavalu, Simona; Youssef, Mahmoud E.; Kaddah, Mohamed M.Y.; Mourad, Ahmed A.E.; Gaafar, Ahmed Gaafar Ahmed; El‐Ahwany, Eman; Amin, Noha A.; Arakeep, Heba M.; Shata, Ahmed; Saleh, Safaa; Hafez, Mohamed M.; Elazab, Sara T.; Abdelhady, Rasha; Shahat, Rehab Mohamed El; Yahya, Galal; Saber, Sameh

doi:10.1016/j.biopha.2022.112723

Cited by 24 publications

(10 citation statements)

References 76 publications

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Section: Methodsmentioning

confidence: 99%

“…Inflammation score was assessed in the H&E slides according to the following criteria: 0, no inflammation; 1, marginal inflammatory-cell infiltration; 2, mild inflammatory-cell infiltration; 3, moderate inflammatory-cell infiltration; 4, severe inflammatory-cell infiltration; and 5, very extreme inflammatory-cell infiltration. 23 Sections of 4 µm thickness were mounted on slides and deparaffinized with xylene and ethanol solutions of different grades. Citrate buffer ( pH = 6) was added for antigen retrieval and slides were blocked using 1% bovine serum albumin.…”

Section: Histopathological and Immunohistochemical Analysesmentioning

confidence: 99%

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Betanin protects against bleomycin-induced pulmonary fibrosis by regulating the NLRP3/IL-1β/TGF-β1 pathway-mediated epithelial-to-mesenchymal transition

Abd Elrazik,

Helmy

2024

Food Funct.

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Section: Methodsmentioning

confidence: 99%

Section: Histopathological and Immunohistochemical Analysesmentioning

confidence: 99%

Betanin protects against bleomycin-induced pulmonary fibrosis by regulating the NLRP3/IL-1β/TGF-β1 pathway-mediated epithelial-to-mesenchymal transition

Abd Elrazik,

Helmy

2024

Food Funct.

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“…The PCR amplification steps were 10 min/95 °C followed by 40 repeated cycles of 20 s/95 °C, 20 s/62 °C, and 65 s/72 °C. The housekeeping ropD gene was used as the reference gene to normalize the expression of quorum sensing genes, and the relative gene expression was calculated by the comparative threshold cycle (ΔΔCt) method, as described earlier [ 60 , 66 , 67 , 68 ]. The experiment was completed in triplicate, and the expression of caraway oil-treated bacterial genes was presented as mean relative to untreated PAO1 controls.…”

Section: Methodsmentioning

confidence: 99%

GC-MS Analysis and Microbiological Evaluation of Caraway Essential Oil as a Virulence Attenuating Agent against Pseudomonas aeruginosa

et al. 2022

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The emergence of resistant microbes threatens public health on our planet, and the emergence of resistant bacteria against the most commonly used antibiotics necessitates urgent alternative therapeutic options. One way to fight resistant microbes is to design new antimicrobial agents, however, this approach takes decades of research. An alternative or parallel approach is to target the virulence of bacteria with natural or synthetic agents. Active constituents from medicinal plants represent a wide library to screen for natural anti-virulence agents. Caraway is used as a traditional spice and in some medicinal applications such as carminative, antispasmodic, appetizer, and expectorant. Caraway essential oil is rich in terpenes that were previously reported to have antimicrobial activities. In our study, we tested the caraway essential oil in sub-inhibitory concentration as a virulence agent against the Gram-negative bacteria Pseudomonas aeruginosa. Caraway essential oil in sub-inhibitory concentration dramatically blocked protease activity, pyocyanin production, biofilm formation, and quorum sensing activity of P. aeruginosa. The gas chromatography–mass spectroscopy (GC-MS) profile of caraway fruit oil identified 13 compounds representing 85.4% of the total oil components with carvone and sylvestrene as the main constituents. In conclusion, caraway essential oil is a promising virulence-attenuating agent that can be used against topical infections caused by P. aeruginosa.

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“…Primer sequences were as follows: RANKL, F 5′-CGCTCTGTTCCTGTACTTTCGAGCG-3′ and R 5′-TCGTGCTCCCTCCTTTCATCAGGTT-3′; TLR4, F 5′-CGCTTTCACCTCTGCCTTCACTACAG-3′ and R 5′-ACACTACCACAATAACCTTCCGGCTC-3′; β-actin, F 5′-GATTACTGCTCTGGCTCCTAGC-3′ and R 5′-GACTCATCGTACTCCTGCTTGC-3′. Relative mRNA expression levels were normalized to the housekeeping β-actin mRNA in the same sample using the 2 −ΔΔCq method according to [ 65 , 66 ].…”

Section: Methodsmentioning

confidence: 99%

Ameliorative Effect of Dabigatran on CFA-Induced Rheumatoid Arthritis via Modulating Kallikrein-Kinin System in Rats

Youssef

Abdel-Reheim

Morsy

et al. 2022

IJMS

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Rheumatoid arthritis is an autoimmune disease that affects joints, leading to swelling, inflammation, and dysfunction in the joints. Recently, research efforts have been focused on finding novel curative approaches for rheumatoid arthritis, as current therapies are associated with adverse effects. Here, we examined the effectiveness of dabigatran, the antithrombotic agent, in treating complete Freund’s adjuvant (CFA)-induced arthritis in rats. Subcutaneous injection of a single 0.3 mL dosage of CFA into the rat’s hind leg planter surface resulted in articular surface deformities, reduced cartilage thickness, loss of intercellular matrix, and inflammatory cell infiltration. There were also increased levels of the Anti-cyclic citrullinated peptide antibody (ACPA), oxidative stress, and tissue Receptor activator of nuclear factor–kappa B ligand (RANKL). Proteins of the kallikrein-kinin system (KKS) were also elevated. The inhibitory effects of dabigatran on thrombin led to a subsequent inhibition of KKS and reduced Toll-like receptor 4 (TLR4) expression. These effects also decreased RANKL levels and showed anti-inflammatory and antioxidant effects. Therefore, dabigatran could be a novel therapeutic strategy for arthritis.

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Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need

Cited by 24 publications

References 76 publications

Betanin protects against bleomycin-induced pulmonary fibrosis by regulating the NLRP3/IL-1β/TGF-β1 pathway-mediated epithelial-to-mesenchymal transition

Betanin protects against bleomycin-induced pulmonary fibrosis by regulating the NLRP3/IL-1β/TGF-β1 pathway-mediated epithelial-to-mesenchymal transition

GC-MS Analysis and Microbiological Evaluation of Caraway Essential Oil as a Virulence Attenuating Agent against Pseudomonas aeruginosa

Ameliorative Effect of Dabigatran on CFA-Induced Rheumatoid Arthritis via Modulating Kallikrein-Kinin System in Rats

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