Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin

McGhie, Emma J.; Hayward, Richard; Koronakis, Vassilis

doi:10.1093/emboj/20.9.2131

Cited by 169 publications

(169 citation statements)

References 34 publications

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“…After fusion of macrophage lysosomes with phagosomes, the S. enteritidis appears in the phagocytotic vesicle of the cytoplasm [29] , and may destroy the cell invagination. In the present study, there are different reports about the S. enteritidis entering the cell, the bulk of the investigations [30,31] . Scientists report recently that the dendritic cell (DC) residing in the mucosa of the small intestine can use the dendrite to open the tight junction of the cells, and directly ingest the bacteria from the mucosa [32] .…”

Section: The Value Of S Enteritidis Infections From Other Viral Infementioning

confidence: 79%

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Yan

Cheng²,

Wang³

et al. 2008

WJG

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CONCLUSION:IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis . We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium. INTRODUCTIONSalmonella enteritidis (S. enteritidis) is the infectious disease causing Zoonoses. S. enteritidis is one of the primary causes of human food poisoning throughout the world, and has become a pointed public health problem [1][2][3][4] . China is the biggest country in the raising and consumption of duck in the world, but the S. enteritidis bacillus infection is a severely important infectious disease in the duck industry [5] . S. enteritidis outbreaks have been found to be associated with the consumption of contaminated and undercooked poultry products, such as eggs and egg-containing products, and have become a serious economic and public health problem [6] . Conventional methods for isolation of S. enteritidis are too laborious, not sufficiently sensitive or correct [7,8] . To our knowledge, a serum method for detection and antigen location of S. enteritidis has not been reported. Thus, to establish a rapid, sensitive and highly specific in paraffin slices and antigen location in infected duck tissues. METHODS:Rabbits were immunized with purified bacillus to obtain S. enteritidis -specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. S. enteritidis was detected in each organ tissue of ducklings experimentally infected with S. enteritidis . RESULTS:The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen was mainly distributed in the infected cell cytoplasm.

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Section: The Value Of S Enteritidis Infections From Other Viral Infementioning

confidence: 79%

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Yan

Cheng²,

Wang³

et al. 2008

WJG

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“…Considering that two InvB can bind per SipA (26,27), we estimated that SipA can reach local concentrations sufficient for remodeling the host cell actin cytoskeleton in vitro and in vivo within 16-25 sec after docking (5,7,18,28). SopE is injected with kinetics similar to those of SipA, but it does not accumulate in foci (Fig.…”

Section: Resultsmentioning

confidence: 99%

Real-time imaging of type III secretion: Salmonella SipA injection into host cells

Schlumberger¹,

Müller²,

Ehrbar³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

182

200

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Many pathogenic and symbiotic Gram-negative bacteria employ type III secretion systems to inject ''effector'' proteins into eukaryotic host cells. These effectors manipulate signaling pathways to initiate symbiosis or disease. By using time-lapse microscopy, we have imaged delivery of the Salmonella type III effector protein SipA͞SspA into animal cells in real time. SipA delivery mostly began 10 -90 sec after docking and proceeded for 100 -600 sec until the bacterial SipA pool (6 ؎ 3 ؋ 10 3 molecules) was exhausted. Similar observations were made for the effector protein SopE. This visualization of type III secretion in real time explains the efficiency of host cell manipulation by means of this virulence system. effector protein ͉ time-lapse microscopy

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“…At least six effectors induce remodeling of actin cytoskeleton: SipA, SipC, SopB, SopE, SopE2, and SptP. SipA and SipC directly bind to actin, cooperate to promote the formation of actin filaments at the site of bacterial adhesion, and prevent filament disassembly by host factors [20]. SipA modulates the actin-bundling activity of T-plastin [21] and inhibits the filament-depolymerizing factor ADF/cofilin and the filament capping and severin protein gelsolin [22].…”

Section: Actin Cytoskeleton and Invasionmentioning

confidence: 99%

Impact ofSalmonella entericaType III Secretion System Effectors on the Eukaryotic Host Cell

Ramos‐Morales

2012

ISRN Cell Biology

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Type III secretion systems are molecular machines used by many Gram-negative bacterial pathogens to inject proteins, known as effectors, directly into eukaryotic host cells. These proteins manipulate host signal transduction pathways and cellular processes to the pathogen's advantage. Salmonella enterica possesses two virulence-related type III secretion systems that deliver more than forty effectors. This paper reviews our current knowledge about the functions, biochemical activities, host targets, and impact on host cells of these effectors. First, the concerted action of effectors at the cellular level in relevant aspects of the interaction between Salmonella and its hosts is analyzed. Then, particular issues that will drive research in the field in the near future are discussed. Finally, detailed information about each individual effector is provided.

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Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin

Cited by 169 publications

References 34 publications

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues

Real-time imaging of type III secretion: Salmonella SipA injection into host cells

Impact ofSalmonella entericaType III Secretion System Effectors on the Eukaryotic Host Cell

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