Cooperative clinical trial of photodynamic therapy with photofrin II and excimer dye laser for early gastric cancer

Mimura, Seishiro; Ito, Yoshiaki; Nagayo, Takeo; Ichii, Makoto; Kato, Harubumi; Sakai, Harumasa; Gotō, Kazuo; Noguchi, Yasuki; Tanimura, Hiroshi; Nagai, Yugo; Suzuki, Shigeru; Hiki, Yoshiki; Hayata, Yoshihiro

doi:10.1002/(sici)1096-9101(1996)19:2<168::aid-lsm7>3.0.co;2-q

Cited by 68 publications

(17 citation statements)

References 4 publications

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“…5-ALA, is effective in several types of cancers (12)(13)(14). These porphyrin compounds of HpD and photofrin have been shown to act as radiosensitizers, yet the radiosensitizing effect of 5-ALA is controversial (15)(16)(17).…”

Section: -Aminolevulinic Acid Strongly Enhances Delayed Intracellulamentioning

confidence: 99%

5-Aminolevulinic acid strongly enhances delayed intracellular production of reactive oxygen species (ROS) generated by ionizing irradiation: Quantitative analyses and visualization of intracellular ROS production in glioma cells in vitro

et al. 2014

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Abstract. Postoperative adjuvant radiotherapy has important roles in multimodal treatment for highly aggressive malignant gliomas. Previously, we demonstrated that multi-dose ionizing irradiation with repetitive administration of 5-aminolevulinic acid (5-ALA) enhanced the host antitumor response and strongly inhibited tumor growth in experimental glioma. However, the mechanism of the radiosensitizing effect of 5-ALA is not known. Ionizing irradiation not only causes reactive oxygen species (ROS) formation initially by water radiolysis but also induces delayed production of mitochondrial ROS for mediating the long-lasting effects of ionizing irradiation on tumor cells. 5-ALA leads to high accumulation of protoporphyrin IX (PpIX) in the mitochondria of tumor cells, yet can also improve dysfunction of the mitochondrial respiratory chain in tumor cells. Here, we assessed the effect of 5-ALA-induced PpIX synthesis and delayed production of intracellular ROS after ionizing irradiation with 5-ALA in glioma cells in vitro. Temporal changes in intracellular 5-ALA-induced PpIX synthesis after ionizing irradiation in glioma cell lines were evaluated using flow cytometry (FCM). Then, the effect of 5-ALA on delayed production of intracellular ROS 12 h after ionizing irradiation in glioma cells was evaluated by FCM and confocal laser scanning microscopy. Ionizing irradiation had no effect on 5-ALA-induced PpIX synthesis in glioma cells. Delayed intracellular production of ROS was significantly higher than that just after ionizing irradiation, but 5-ALA pretreatment strongly enhanced the delayed intracellular production of ROS, mainly in the cytoplasm of glioma cells. This 5-ALA-induced increase in the delayed production of ROS tended to be higher in the case of 5-ALA treatment before rather than after ionizing irradiation. These results suggest that 5-ALA can affect tumor cells under ionizing irradiation, and greatly increase secondary intracellular production of ROS long after ionizing irradiation, thereby causing a radiosensitizing effect in glioma cells.

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Section: -Aminolevulinic Acid Strongly Enhances Delayed Intracellulamentioning

confidence: 99%

5-Aminolevulinic acid strongly enhances delayed intracellular production of reactive oxygen species (ROS) generated by ionizing irradiation: Quantitative analyses and visualization of intracellular ROS production in glioma cells in vitro

et al. 2014

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“…Photodynamic therapy (PDT) is an effective treatment method for several kinds of neoplasms and is based on the use of different photosensitizers (3)(4)(5)(6). In general, the effectiveness of a photosensitizer depends on two important properties, namely the production of cytotoxic oxygen and fluorescence excited by laser irradiation, which are important for PDT and fluorescence-guided resection in neurosurgery (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Radiosensitizing effect of 5-aminolevulinic acid-induced protoporphyrin IX in glioma cells inï¿½vitro

et al. 2012

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Abstract. 5-Aminolevulinic acid (ALA) is a prodrug used in photodynamic therapy and fluorescence-guided resection of malignant gliomas due to its high cellular uptake in tumours. Porphyrin compounds act not only as photosensitizers but also as radiosensitizers. In the present study, the possible use of 5-ALA as a radiosensitizer for malignant gliomas was examined in vitro. Rat glioma cell lines (9L, C6) were pre-treated with 5-ALA and exposed to ionizing irradiation. The radiosensitizing effect of 5-ALA was evaluated by colony-forming assay. Intracellular reactive oxygen species (ROS) produced by 5-ALA and irradiation were evaluated by confocal laser scanning microscopy. Pre-treatment with 5-ALA enhanced the radiosensitivity of 9L cells to single-dose ionizing irradiation compared with controls (D 0 value, 4.35±0.20 and 4.84±0.23 Gy, respectively, P≤0.05). Exposure to multi-dose ionizing irradiation revealed high radiosensitivity in both 9L and C6 cells pre-treated with 5-ALA compared to controls. Production of intracellular ROS increased in 9L cells pretreated with 5-ALA after ionizing irradiation compared to control cells. Thus, 5-ALA functions as a specific radiosensitizer for malignant gliomas. Intracellular 5-ALA-induced PpIX plays an important role in the production of ROS and the radiosensitizing effect under ionizing irradiation conditions.

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“…In several studies in Japan, The largest series by Mimura et al 17 included 27 patients with early gastric cancer: After PDT using photoprin administration two days before procedure, the complete remission rate was 88%, whereas a smaller study including seven patients using same phortsensitizer, showed 100% of complete remission rate. Another study using 2nd generation photosensitizer, the rate of complete remission was 73%.…”

Section: Clinical Datamentioning

confidence: 99%

The Effects of Photodynamic Therapy in Upper-Gastrointestinal Malignant Diseases

Kim

Park

2010

Gut Liver

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Photodynamic therapy (PDT) is a promising new modality that utilizes the combination of a photosensitizing chemical and visible light for the management of various solid malignancies, including gastrointestinal (GI) cancer. PDT has some advantages over chemotherapy in terms of its greater safety and lower toxicity in the treatment of malignant lesions. However, PDT has not been used widely for treating upper GI cancer due to its relatively low cost-effectiveness and anatomical characteristics of the GI system. Nevertheless, PDT may be an effective alternative therapy for early upper-GI cancer patients who are at a high risk of curative surgical resection or systemic chemotherapy. In some clinical studies, PDT for various upper GI cancer showed positiveresults. To improve the efficacy of PDT for upper GI cancer, development of photosensitezer and light delivery system is needed.

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Cooperative clinical trial of photodynamic therapy with photofrin II and excimer dye laser for early gastric cancer

Cited by 68 publications

References 4 publications

5-Aminolevulinic acid strongly enhances delayed intracellular production of reactive oxygen species (ROS) generated by ionizing irradiation: Quantitative analyses and visualization of intracellular ROS production in glioma cells in vitro

5-Aminolevulinic acid strongly enhances delayed intracellular production of reactive oxygen species (ROS) generated by ionizing irradiation: Quantitative analyses and visualization of intracellular ROS production in glioma cells in vitro

Radiosensitizing effect of 5-aminolevulinic acid-induced protoporphyrin IX in glioma cells inï¿½vitro

The Effects of Photodynamic Therapy in Upper-Gastrointestinal Malignant Diseases

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