2015
DOI: 10.1128/jvi.00438-15
|View full text |Cite
|
Sign up to set email alerts
|

Cooperativity of HIV-Specific Cytolytic CD4 T Cells and CD8 T Cells in Control of HIV Viremia

Abstract: CD4؉

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

9
79
1

Year Published

2016
2016
2018
2018

Publication Types

Select...
5
1
1

Relationship

1
6

Authors

Journals

citations
Cited by 75 publications
(90 citation statements)
references
References 38 publications
9
79
1
Order By: Relevance
“…ANOVA (A-D and H), paired t test (F), *P < 0.05, **P < 0.005, ***P < 0.0005. jci.org Volume 126 Number 10 October 2016 expression was quantified in both CD107a + and CD107 -cells as an expression threshold above background. These degranulatory CD107a + HIV-responding (IFN-γ + ) CD4 T cells highly expressed granzyme B mRNA, further confirming their cytolytic function ( Figure 7C), similar to previously published data associating CD4 CTL with CD107, granyzme, and EOMES protein expression (43). To determine whether TGF-β signaling similarly regulated granzyme and EOMES in HIV-specific CD4 T cells, HIV-responsive CD4 T cells were expanded with GAG peptide pools in the presence or absence of recombinant human TGF-β (rhTGF-β) for 5 days, rested, and restimulated with peptides and anti-CD28 and anti-CD49d mAbs in the presence of monensin for 5 hours and then analyzed for cytolytic markers.…”
Section: Cd4 T Cells From Ezh2supporting
confidence: 76%
See 2 more Smart Citations
“…ANOVA (A-D and H), paired t test (F), *P < 0.05, **P < 0.005, ***P < 0.0005. jci.org Volume 126 Number 10 October 2016 expression was quantified in both CD107a + and CD107 -cells as an expression threshold above background. These degranulatory CD107a + HIV-responding (IFN-γ + ) CD4 T cells highly expressed granzyme B mRNA, further confirming their cytolytic function ( Figure 7C), similar to previously published data associating CD4 CTL with CD107, granyzme, and EOMES protein expression (43). To determine whether TGF-β signaling similarly regulated granzyme and EOMES in HIV-specific CD4 T cells, HIV-responsive CD4 T cells were expanded with GAG peptide pools in the presence or absence of recombinant human TGF-β (rhTGF-β) for 5 days, rested, and restimulated with peptides and anti-CD28 and anti-CD49d mAbs in the presence of monensin for 5 hours and then analyzed for cytolytic markers.…”
Section: Cd4 T Cells From Ezh2supporting
confidence: 76%
“…Therefore, we aimed to investigate whether TGF-β could also regulate cytotoxicity-associated molecules in purified human CD4 T cells from HIV-infected patients (Supplemental Table 1). After in vitro stimulation with a pool of GAG peptides, HIV-responding IFN-γ + CD107a + CD4 + T cells were isolated using a previously described capture method (43). Using single-cell index sorting and Fluidigm Biomark Technology, we found that Eomes mRNA was highly associated with HIV-responsive degranulatory activity (CD107a protein) in CD4 T cells ( Figure 7C).…”
Section: Cd49dmentioning
confidence: 98%
See 1 more Smart Citation
“…For example, factors including sex, age [11,12], race [5,10], diurnal rhythms [8,22], physical and psychological stress, pregnancy, drug administration [9], tuberculosis, viral infections [16], presence of anti-lymphocyte auto antibodies and procedures such as splenectomy or strenuous physical activities [13,23] have been shown to influence CD4 T lymphocyte counts in individuals. In addition, the role and characteristics of CD4 T lymphocyte cells in varied human viral infections including HIV [14] and hepatitis viruses [2, 3, 15-17, 19, 20] have been reported.…”
Section: Discussionmentioning
confidence: 99%
“…Specifically, infection with some viral pathogens including HIV [14] and hepatitis viruses [2,[15][16][17] havebeennoted to influencecharacteristics ofCD4 T lymphocyte cells.…”
Section: Cc-by-nc-ndmentioning
confidence: 99%