Coordination polymer nanoprobe integrated carbon dot and phenol red for turn-on fluorescence detection of urease activity

Li, Sheng‐Hua; Xiao, Lingyu; Xiao, Long-Qian; Tan, Hongliang

doi:10.1007/s00604-023-05644-y

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…5,6 Thus, monitoring of urease is of great importance for both bioanalysis and environmental safety. To date, the main methods commonly used for urease detection are fluorometry, 7 colorimetry, 8 surface-enhanced Raman scattering (SERS), and electrochemistry. 9,10 These methods are based on a single-signal instrument output, which makes it difficult to achieve rapid and accurate in situ detection.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Urease, which is widespread in microorganisms, plants, and animals, specifically catalyzes the hydrolysis of urea to produce NH 3 and CO 2 , , and has important roles in disease diagnosis, the environment, and agricultural production. , While urease in the soil and environment has been linked to the loss of agricultural nitrogen fertilizers and ammonia emissions from microorganisms. , Thus, monitoring of urease is of great importance for both bioanalysis and environmental safety. To date, the main methods commonly used for urease detection are fluorometry, colorimetry, surface-enhanced Raman scattering (SERS), and electrochemistry. , These methods are based on a single-signal instrument output, which makes it difficult to achieve rapid and accurate in situ detection. Therefore, it is crucial to establish a portable urease assay with multiple signal outputs.…”

Section: Introductionmentioning

confidence: 99%

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Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Li,

Xie,

Zhang

et al. 2024

Anal. Chem.

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In this work, a novel nanozyme (Cu@Zr) with all-in-one dual enzyme and fluorescence properties is designed by simple self-assembly. A nanozyme cascade sensor with disodium phenyl phosphate (PPDS) as substrate was first established by exploiting the dual enzymatic activities of phosphatase and laccase. Specifically, phosphatase cleaves the P–O bond of PPDS to produce colorless phenol, which is then oxidized by laccase and complexed with the chromogenic agent 4-aminoantipyrine (4-AP) to produce red quinoneimine (QI). Strikingly, the NH3 produced by the urease hydrolysis of urea can interact with Cu@Zr, accelerating the electron transfer rate and ultimately leading to a significantly improved performance of the cascade reaction. Moreover, the fluorescence at 440 nm of Cu@Zr is further quenched by the inner filter effect (IFE) of QI. Thus, the colorimetric and fluorescence dual-mode strategy for sensitive urease analysis with LODs of 3.56 and 1.83 U/L was established by the proposed cascade sensor. Notably, a portable swab loaded with Cu@Zr was also prepared for in situ urease detection with the aid of a smartphone RGB readout. It also provides a potentially viable analytical avenue for environmental and biological analysis.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Li,

Xie,

Zhang

et al. 2024

Anal. Chem.

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“…A variety of transition metal complexes (TMCs) serving as UIs have been identified in recent years. − Cu-based TMCs show stronger urease inhibitory efficacy than other metal-based TMCs . Meanwhile, coordination polymers (CPs) are a class of hybrid materials composed of organic linkers and bridging metal ions or clusters. − They have variable pore size, a high specific surface area, and strong biocompatibility. − The CP contains both metal and organic ligands and exhibits excellent water stability, making it suitable as a UI .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Substituent-Regulated Two-Dimensional Copper-Based Coordination Polymers as Urease Inhibitors

Duan,

Li,

Yan

et al. 2024

Crystal Growth & Design

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This article reports the design and synthesis of two copper-based coordination polymers (Cu-CPs) constructed from mixed ligands containing a bis-pyridine-bis-amide group and trimellitic acid. By using substituent-regulated acids, the simplified two-dimensional (2D) double layers for the Cu-CP-1 and Cu-CP-2 possess different grid windows, which can be served as a kind of urease inhibitors (UIs) and their inhibition mechanisms were explored in details. The results show that both Cu-CPs exhibited good urease inhibitory activity, and the inhibitory activities of all derivatives were higher than that of the standard thio urea, with UI-2 exhibiting better urease inhibitory performance. To better understand the potential mechanism of urease inhibition by these CPs, enzyme kinetics analysis was performed using Lineweaver− Burk (L−B) plots at different inhibitor concentrations, which indicated that UI-1 and UI-2 exhibited noncompetitive inhibitory nature. Additionally, molecular docking confirmed the binding interactions of UI-1 and UI-2 with the active site of urease. The urease inhibition experiments were successfully verified, and the effects of the structures of CPs on urease inhibition were measured through DFT calculations.

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The colorful world of sulfonephthaleins: Current applications in analytical chemistry for “old but gold” molecules

Magnaghi,

Zanoni,

Alberti

et al. 2023

Analytica Chimica Acta

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Coordination polymer nanoprobe integrated carbon dot and phenol red for turn-on fluorescence detection of urease activity

Cited by 7 publications

References 33 publications

Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Fabrication of Substituent-Regulated Two-Dimensional Copper-Based Coordination Polymers as Urease Inhibitors

The colorful world of sulfonephthaleins: Current applications in analytical chemistry for “old but gold” molecules

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