Copine1 Enhances Neuronal Differentiation of the Hippocampal Progenitor HiB5 Cells

Park, Nammi; Yoo, Jae Cheal; Ryu, Jiwon; Hong, Seong Geun; Hwang, Eun Mi; Park, Jae Yong

doi:10.1007/s10059-012-0235-7

Cited by 31 publications

(29 citation statements)

References 23 publications

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“…We previously reported a screening system for genes involved in neuronal differentiation and found that CPNE1 enhanced the neuronal differentiation of HiB5 cells [14,16]. Therefore, we questioned which domain of CPNE1 was related with neuronal differentiation.…”

Section: Discussionmentioning

confidence: 97%

“…We previously reported that CPNE1 increases Akt phosphorylation, neuronal marker protein (Tuj1) levels, and neurite outgrowth in HiB5 cells, indicating neuronal differentiation [14]. To determine which region of CPNE1 is related with neuronal differentiation, we constructed adenoviruses containing GFP-CPNE1, -C2A, -C2B, and -A domain mutants (Fig.…”

Section: Effect Of Truncated Cpne1 Mutants On Neuronal Progenitor Hibmentioning

confidence: 99%

“…HiB5 cells are a hippocampal progenitor cell line derived from the hippocampal anlagen of embryonic day 16 rat embryos that was conditionally immortalized with a temperature-sensitive mutant allele of SV40 T antigen [15]. In our previous study, we found that CPNE1 enhanced HiB5 neuronal differentiation not through the phosphoinositide 3-kinase (PI3K)-Akt pathway but through another Akt activated signaling pathway [14]. Here, we provide the first evidence that neuronal differentiation of HiB5 cells induced by CPNE1 is related to its C2 domains and not its A domain.…”

Section: Introductionmentioning

confidence: 98%

“…Recently, Akt was reported to be a key mediator of neurite outgrowth and also promotes neuronal survival [13]. CPNE1 phosphorylates Akt on serine residue 473 (S473) during HiB5 cell neuronal differentiation [14]. HiB5 cells are a hippocampal progenitor cell line derived from the hippocampal anlagen of embryonic day 16 rat embryos that was conditionally immortalized with a temperature-sensitive mutant allele of SV40 T antigen [15].…”

Section: Introductionmentioning

confidence: 99%

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Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

Park

Yoo

Choi

et al. 2014

Biochemical and Biophysical Research Communications

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Section: Discussionmentioning

confidence: 97%

Section: Effect Of Truncated Cpne1 Mutants On Neuronal Progenitor Hibmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

Park

Yoo

Choi

et al. 2014

Biochemical and Biophysical Research Communications

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“…The rat hippocampal progenitor cell line, HiB5, was grown as previously reported (Park et al, 2012). Briefly, the cells were maintained at 33°C (their permissive temperature) in DMEM (GIBCO BRL, USA) containing penicillin G (100 U/ml; GIBCO BRL), streptomycin sulfate (100 μg/ml; GIBCO BRL), and 10% fetal bovine serum (GIBCO BRL).…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

The Molecular Mechanism of NELL2 Movement and Secretion in Hippocampal Progenitor HiB5 Cells

Hwang

Kim

et al. 2013

Molecules and Cells

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Neural epidermal growth factor-like protein-like 2 (NELL2) is a secreted glycoprotein that is predominantly expressed in the nervous system, but little is known about the intracellular movement and secretion mechanism of this protein. By monitoring the localization and movements of enhanced green fluorescent protein (EGFP)-labeled NELL2 in living cultured hippocampal neuroprogenitor HiB5 cells, we determined the subcellular localization of NELL2 and its intracellular movement and secretion mechanism. Cterminal EGFP-fused NELL2 showed a typical expression pattern of secreted proteins, especially with respect to its localization in the endoplasmic reticulum, Golgi apparatus, and punctate structures. Vesicles containing NELL2 exhibited bidirectional movement in HiB5 cells. The majority of the vesicles (70.1%) moved in an anterograde direction with an average velocity of 0.454 μm/s, whereas some vesicles (28.7%) showed retrograde movement with an average velocity of 0.302 μm/s. The movement patterns of NELL2 vesicles were dependent upon the presence of microtubules in HiB5 cells. Anterograde movement of NELL2 did not lead to a detectable accumulation of NELL2 in the peripheral region of the cell, indicating that it was secreted into the culture medium. We also showed that the N-terminal 29 amino acids of NELL2 were important for secretion of this protein. Taken together, these results strongly suggest that the N-terminal region of NELL2 determines both the pattern of its intracellular expression and transport of NELL2 vesicles by high-velocity movement. Therefore, NELL2 may affect the cellular activity of cells in a paracrine or autocrine manner.

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Role of CPNE1 in Odontoblastic Differentiation of Rat Stem Cells from Apical Papilla

Xie

et al. 2023

Advanced Biology

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CPNE1 is a calcium‐dependent, phospholipid‐binding protein that is ubiquitously expressed in various tissues and organs. This study investigates the expression and localization of CPNE1 in tooth germ development and the role of CPNE1 in odontoblastic differentiation. In rat tooth germs, CPNE1 is expressed in the odontoblasts and ameloblasts since the late bell stage. The depletion of CPNE1 in the stem cells from apical papilla (SCAPs) clearly inhibits the expression of odontoblastic‐related genes and the formation of mineralized nodules during differentiation, while CPNE1 overexpression promotes this process. In addition, CPNE1 overexpression increases AKT phosphorylation during the odontoblastic differentiation of SCAPs. Furthermore, treatment with AKT inhibitor (MK2206) reduces the expression of odontoblastic‐related genes in CPNE1 over‐expressed SCAPs, and Alizarin Red staining shows reduced mineralization. These results suggest that CPNE1 plays a role in the tooth germ development as well as the odontblastic differentiation of SCAPs in vitro that is related to the AKT signaling pathway.

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Copine1 Enhances Neuronal Differentiation of the Hippocampal Progenitor HiB5 Cells

Cited by 31 publications

References 23 publications

Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells

The Molecular Mechanism of NELL2 Movement and Secretion in Hippocampal Progenitor HiB5 Cells

Role of CPNE1 in Odontoblastic Differentiation of Rat Stem Cells from Apical Papilla

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