Copolymers of amine methacrylate with poly(ethylene glycol) as vectors for gene therapy

Rungsardthong, Uracha; Deshpande, Mangesh C.; Bailey, L A.; Vamvakaki, Maria; Armes, Steven P.; Garnett, Martin C.; Stolnik, Snow

doi:10.1016/s0168-3659(01)00295-4

Cited by 128 publications

(113 citation statements)

References 39 publications

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“…For P60, toxicity was almost the same at low concentration, but there was a 3-fold lower toxicity than for PDMAEMA at high concentration, such as 20 mg L -1 . A similar phenomenon has been reported before for other polymers [18,23,24]. Due to the biocompatibility of PVP [32], cytotoxicity can be expected based on the DMAEMA content of the polymer.…”

Section: Cytotoxicitysupporting

confidence: 85%

“…6c). A similar spherical morphology has recently been reported using amine methacrylate-based systems and PEG copolymers [22,36]. The reasons for the small size and uniform complexes formed using PVP grafted copolymer could be explained by a micelle-like structure.…”

Section: Morphology Of Polymer/dna Complexessupporting

confidence: 81%

“…The EtBr displacement assay provides an assessment of the polycations' ability to form complexes with DNA by measuring changes in the fluorescence of EtBr-DNA complexes. Loss of EtBr fluorescence is thought to the result of polyelectrolyte binding to DNA-polymer complexes, resulting in expulsion of intercalated EtBr molecules [36]. In this study, the assay is used to assess the ability of the graft copolymers to bind to DNA (Fig.…”

Section: Ethidium Bromide Displacement Assaysmentioning

confidence: 99%

“…To overcome these drawbacks, non-charged hydrophilic segments have been introduced as an effective modification of synthetic cationic polymeric DNA delivery systems [23][24][25]. Systems such as PEO-b-PDMAEMA [26], Pluronic-b-PDMAEMA [27], PEG-modified PDMAEMA [28] and PDMAEMA-b-PMPC (poly (methacryloyloxyethyl phosphorylcholine)) [29] have been widely studied as potential gene delivery systems.…”

Section: Introductionmentioning

confidence: 99%

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Polycationic graft copolymers of poly(N-vinylpyrrolidone) as non-viral vectors for gene transfection

Liu¹,

Cao²,

Dao-feng³

et al. 2009

Open Chemistry

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Novel graft copolymers of 2-(dimethylamino)ethyl methacrylate (DMAEMA) with N-vinylpyrrolidone (NVP) were designed and synthesized by the free radical copolymerization of DMAEMA with precursor polymers of vinyl-functionalized poly(N-vinylpyrrolidone) (PVP). The ability of the PVP-grafted copolymers to bind and condense DNA was confirmed by ethidium bromide displacement assay, agarose gel electrophoresis and transmission electron microscopy. The presence of PVP in the copolymers had a favorable effect on the biophysical properties of polymer/DNA complexes. Colloidal stable complexes obtained from the copolymer systems, were shown to be separate, uniformly spherical nanoparticles by transmission electron microscopy. The approximate diameter of the complexes was 150 -200 nm, as determined by dynamic light scattering studies. These results confirm an important role played by the PVP grafts in producing compact stable DNA complexes. The ζ-potential measurements revealed that the incorporation of the PVP grafts reduced the positive surface charge of polymer/DNA complexes. The cytotoxicity of the copolymers decreased with an increasing fraction of PVP. Furthermore, in vitro transfection experiments with these copolymers showed improved ability of transfection in cell culture, demonstrating an important role for PVP grafts in enhancement of the transfection efficiency.© Versita Warsaw and Springer-Verlag Berlin Heidelberg.

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Section: Cytotoxicitysupporting

confidence: 85%

Section: Morphology Of Polymer/dna Complexessupporting

confidence: 81%

Section: Ethidium Bromide Displacement Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polycationic graft copolymers of poly(N-vinylpyrrolidone) as non-viral vectors for gene transfection

Liu¹,

Cao²,

Dao-feng³

et al. 2009

Open Chemistry

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show abstract

“…[6] Moreover, Kabanov et al have demonstrated that micellization can be induced by adding either a cationic homopolyelectrolyte (quaternized poly(4-vinylpyridine)) or a cationic surfactant (hexadecyltrimethylammonium bromide) to an aqueous solution of a molecularly dissolved neutralanionic AB diblock copolymer, for example, poly(ethylene oxide-block-sodium methacrylate). [7] Both Harada and Kataoka and also Jerome's group have reported similar observations, [8] and very recently Schlaad and co-workers have exploited polyelectrolyte complexation in non-aqueous media to prepare vesicles.…”

mentioning

confidence: 99%