Copper-Alginate Encapsulation of Crude laccase from Lentinus polychrous Lev. and their Effectiveness in Synthetic Dyes Decolorizations

Phetsom, Jirapa; Khammuang, Saranyu; Suwannawong, Pimjai; Sarnthima, Rakrudee

doi:10.3923/jbs.2009.573.583

Cited by 31 publications

(8 citation statements)

References 9 publications

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“…Entrapment is identified as the simplest immobilization technique in which enzyme molecules disperse into a porous solid matrix; hence no direct attachment may be formed between carrier and enzyme ( Fernández-Fernández et al, 2013 ; Karthik et al, 2021 ). Alginate, collagen, silicon rubber, gelatin, carrageenan, polyurethane, polyacrylamide, and polyvinyl alcohol with styryl pyridinium groups are solid matrices that can be used for enzyme entrapment ( Dayaram and Dasgupta, 2008 ; Phetsom et al, 2009 ; Fernández-Fernández et al, 2013 ). Enzyme entrapment can be carried out in two steps: first enzyme molecules are dispersed into monomer solution, and then a polymerization process ensues which maintains enzyme molecules trapped ( Karthik et al, 2021 ).…”

Section: Immobilization Of Laccasementioning

confidence: 99%

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Adamian

Lonappan

Alokpa

et al. 2021

Front. Bioeng. Biotechnol.

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Τhe ligninolytic enzyme laccase has proved its potential for environmental applications. However, there is no documented industrial application of free laccase due to low stability, poor reusability, and high costs. Immobilization has been considered as a powerful technique to enhance laccase’s industrial potential. In this technology, appropriate support selection for laccase immobilization is a crucial step since the support could broadly affect the properties of the resulting catalyst system. Through the last decades, a large variety of inorganic, organic, and composite materials have been used in laccase immobilization. Among them, carbon-based materials have been explored as a support candidate for immobilization, due to their properties such as high porosity, high surface area, the existence of functional groups, and their highly aromatic structure. Carbon-based materials have also been used in culture media as supports, sources of nutrients, and inducers, for laccase production. This study aims to review the recent trends in laccase production, immobilization techniques, and essential support properties for enzyme immobilization. More specifically, this review analyzes and presents the significant benefits of carbon-based materials for their key role in laccase production and immobilization.

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Section: Immobilization Of Laccasementioning

confidence: 99%

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Adamian

Lonappan

Alokpa

et al. 2021

Front. Bioeng. Biotechnol.

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“…ions [91]. Alginates are also used in the form of beads for immobilization mainly by entrapment of laccases, tyrosinases and various peroxidases.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…For immobilization of oxidoreductases also encapsulation and entrapment are applied. As supports for these techniques of immobilization mainly alginates-based materials are adapted due to their remarkable abilities for gelation under mild conditions and high operational stability [78][79][80][81][82][83][84][85][86][87][88][89][90][91]. Also poly vinyl alcohol beads, silica aerogel and hybrid chitosan/polyacrylamide hydrogel were used for encapsulation of laccases and tyrosinases [102,131,173].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…encapsulation into chitosan beads and by entrapment into copper alginate beads and they were applied for degradation of Indigo carmine [79,91]. Both biocatalytic systems allowed total degradation of the dye, but addition of copper ions into alginate beads increased activity of the laccase and caused that degradation process was four times shorter and was carried out in milder temperature conditions (30 °C) as compared to chitosan beads (37 °C).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

See 1 more Smart Citation

Developments in support materials for immobilization of oxidoreductases: A comprehensive review

Zdarta¹,

Meyer

Jesionowski

et al. 2018

Advances in Colloid and Interface Science

239

147

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Bioremediation, a biologically mediated transformation or degradation of persistent chemicals into nonhazardous or less-hazardous substances, has been recognized as a key strategy to control levels of pollutants in water and soils. The use of enzymes, notably oxidoreductases such as laccases, tyrosinases, various oxygenases, aromatic dioxygenases, and different peroxidases (all of EC class 1) is receiving significant research attention in this regard. It should be stated that immobilization is emphasized as a powerful tool for enhancement of enzyme activity and stability as well as for protection of the enzyme proteins against negative effects of harsh reaction conditions. As proper selection of support materials for immobilization and their performance is overlooked when it comes to comparing performance of immobilized enzyme in academic studies, this review summarizes the current state of knowledge regarding the materials used for enzyme immobilization of these oxidoreductase enzymes for environmental applications. In the presented study, thorough physicochemical characteristics of the support materials was presented. Moreover, various types of reactions and notably operational modes of enzymatic processes for biodegradation of harmful pollutants are summarized, and future trends in use of immobilized oxidoreductases for environmental applications are discussed. Our goal is to provide an improved foundation on which new technological advancements can be made to achieve efficient enzyme-assisted bioremediation.

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“…Chitosan-coated alginate beads have been packed in a fixed bed reactor and employed in RBBR decolourisation, achieving up to 70% decolourisation [ 6 ]. Alginate beads differently modified with chitosan or polyethylene glycol (PEG) have also shown to effectively decolourise several different dyes [ 17 – 19 ]. Entrapment in hydrogel structures has also been applied, for example, in the immobilization of a T. versicolor laccase, resulting in low substrate affinity but improvement in storage stability and in the decolourisation of Acid Orange 52 [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of aPleurotus ostreatusLaccase Mixture on Perlite and Its Application to Dye Decolourisation

Pezzella

Russo

Marzocchella

et al. 2014

BioMed Research International

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In the present study, a crude laccase preparation from Pleurotus ostreatus was successfully immobilized on perlite, a cheap porous silica material, and tested for Remazol Brilliant Blue R (RBBR) decolourisation in a fluidized bed recycle reactor. Results showed that RBBR decolourisation is mainly due to enzyme action despite the occurrence of dye adsorption-related enzyme inhibition. Fine tuning of immobilization conditions allowed balancing the immobilization yield and the resulting rate of decolourisation, with the adsorption capacity of the solid biocatalyst. In the continuous lab scale reactor, a maximum conversion degree of 56.1% was achieved at reactor space-time of 4.2 h. Stability and catalytic parameters of the immobilized laccases were also assessed in comparison with the soluble counterparts, revealing an increase in stability, despite a reduction of the catalytic performances. Both effects are most likely ascribable to the occurrence of multipoint attachment phenomena.

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Copper-Alginate Encapsulation of Crude laccase from Lentinus polychrous Lev. and their Effectiveness in Synthetic Dyes Decolorizations

Cited by 31 publications

References 9 publications

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Recent Developments in the Immobilization of Laccase on Carbonaceous Supports for Environmental Applications - A Critical Review

Developments in support materials for immobilization of oxidoreductases: A comprehensive review

Immobilization of aPleurotus ostreatusLaccase Mixture on Perlite and Its Application to Dye Decolourisation

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