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^Äëíê~Åí= Six agro-industrial wastes were evaluated as a support for ligninolytic enzyme production by the white-rot fungus iÉåíáåìë= éçäóÅÜêçìë Lév. under solid-state fermentation. Enzyme production was markedly different according to the substrate used. Rice bran (RB) yielded the highest laccase activity of 1,449 U/L (after 21 days of culture) with specific activity of 4.4 U/g substrate. Rice bran supplemented with rice husk (RH) (2:1 by wt) showed high laccase activity of 1,425 U/L with specific activity of 10.0 U/g substrate (after 17 days of culture). The crude enzyme of the RH-RB culture also contained manganese peroxidase (MnP) and manganese-independent peroxidase (MIP) activities in relative proportions of 1.9:1.4:1 of laccase:MnP:MIP, respectively. Zymogram studies showed the same isoenzyme pattern with these ligninolytic enzymes. The high enzyme production level and low substrate cost of SSF-iK= éçäóÅÜêçìë Lév. suggest that it has potential for industrial applications. Our studies showed that the crude enzyme from this culture exhibited áå=îáíêç decolorization of Indigo Carmine. The highest efficiency of dye decolorization was observed under alkaline conditions (pH 9.0) at an initial dye concentration of 10 mg/L. The rather high pH conditions and high efficiency in Indigo Carmine decolorization make the enzyme further interest for the applications in treatment of waste water from the textile industry, which contains synthetic dyes. © KSBB hÉóïçêÇëW=ä~ÅÅ~ëÉI=äáÖåáåçäóíáÅ=ÉåòóãÉI Lentinus polychrous i¨î.I=ëçäáÇJëí~íÉ=ÑÉêãÉåí~íáçåI=~ÖêáÅìäíìê~ä=ï~ëíÉëI= = ÇÉÅçäçêáò~íáçå=

Crude proteins of cultured mycelia and fruiting bodies of Ganoderma lucidum were investigated for antioxidant, antibacterial and DNA protective activities. It was found that the half maximal inhibitory concentration (IC 50 ) of the mycelia protein and fruiting bodies protein extracts against 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS •+ ) were 2.47±0.01 and 2.77±0.11 μg protein/ml and against 2,2-diphenylpicrylhydrazyl radical (DPPH • ) were 2.5 ±0.01 and 3.42±0.01 μg protein/ml, respectively. The ferric reducing-antioxidant power (FRAP) values of those samples were 1.73±0.01 and 2.62±0.01 μmole trolox/μg protein respectively. Protein hydrolysates prepared by pronase exhibited a weaker antioxidant activity. Both crude proteins showed antibacterial activity, whereas only the mycelia protein extract could protect DNA damage by hydroxyl (• OH) radicals. This protein extract was partial purified by Diethyl amino ethyl (DEAE)-Sepharose column and Sulfopropyl (SP)-Sepharose column, obtained major protein with molecular weight about 45 kilo Dalton (kDa). In conclusion, G. lucidum protein extracts have promise potential for applications as antioxidant and antibacterial agents.