Crude proteins of cultured mycelia and fruiting bodies of Ganoderma lucidum were investigated for antioxidant, antibacterial and DNA protective activities. It was found that the half maximal inhibitory concentration (IC 50 ) of the mycelia protein and fruiting bodies protein extracts against 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS •+ ) were 2.47±0.01 and 2.77±0.11 μg protein/ml and against 2,2-diphenylpicrylhydrazyl radical (DPPH • ) were 2.5 ±0.01 and 3.42±0.01 μg protein/ml, respectively. The ferric reducing-antioxidant power (FRAP) values of those samples were 1.73±0.01 and 2.62±0.01 μmole trolox/μg protein respectively. Protein hydrolysates prepared by pronase exhibited a weaker antioxidant activity. Both crude proteins showed antibacterial activity, whereas only the mycelia protein extract could protect DNA damage by hydroxyl (• OH) radicals. This protein extract was partial purified by Diethyl amino ethyl (DEAE)-Sepharose column and Sulfopropyl (SP)-Sepharose column, obtained major protein with molecular weight about 45 kilo Dalton (kDa). In conclusion, G. lucidum protein extracts have promise potential for applications as antioxidant and antibacterial agents.

Culture broth of was determined for antioxidant, antibacterial and α-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS and DPPH radicals with a half maximal inhibitory concentration (IC) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively and reducing power equaled to 4.38 ± 0.02 µmol Trolox/µg protein as investigated by ferric ion reducing antioxidant power method. The culture broth experimented into two approached; (1) treated with pronase and (2) filtered through a membrane with 10 kDa molecular weight cut-off (MWCO). The pronase-treated culture broth exhibited insignificant lower antioxidant activities, but the retained culture broth 10 kDa MWCO resulted in significant decrease in antioxidant activities suggesting that the small proteins might play the key role in antioxidant activity. The culture broth could protect DNA damage from hydroxyl radicals (OH) generated by Fenton's reaction. This culture broth showed antibacterial activity towards pathogenic strains and and also had an interesting α-amylase inhibitory activity. This study suggested that apart from the fruiting bodies and the mycelial of , its culture broth also had potential applications as a value-added ingredient in the product such as in cosmetics and in nutraceuticals.