Antioxidant, antibacterial and DNA protective activities of protein extracts from Ganoderma lucidum

Abstract

Crude proteins of cultured mycelia and fruiting bodies of Ganoderma lucidum were investigated for antioxidant, antibacterial and DNA protective activities. It was found that the half maximal inhibitory concentration (IC 50 ) of the mycelia protein and fruiting bodies protein extracts against 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) radical (ABTS •+ ) were 2.47±0.01 and 2.77±0.11 μg protein/ml and against 2,2-diphenylpicrylhydrazyl radical (DPPH • ) were 2.5 ±0.01 and 3.42±0.01 μg protein/ml, respectively. The ferric reducing-antioxidant power (FRAP) values of those samples were 1.73±0.01 and 2.62±0.01 μmole trolox/μg protein respectively. Protein hydrolysates prepared by pronase exhibited a weaker antioxidant activity. Both crude proteins showed antibacterial activity, whereas only the mycelia protein extract could protect DNA damage by hydroxyl (• OH) radicals. This protein extract was partial purified by Diethyl amino ethyl (DEAE)-Sepharose column and Sulfopropyl (SP)-Sepharose column, obtained major protein with molecular weight about 45 kilo Dalton (kDa). In conclusion, G. lucidum protein extracts have promise potential for applications as antioxidant and antibacterial agents.

Antifatigue Functions and Mechanisms of Edible and Medicinal Mushrooms

“…The protein, Ling Zhi-8 (LZ-8), found in G. lucidum extracts was found to help T lymphocytes to respond to cytokines more effectively so as to enhance the immunomodulation effect [ 84 ]. The peptides and crude proteins isolated from G. lucidum were also demonstrated to have antioxidant effect [ 11 , 91 ]. Girjal and coworkers found that the amino acid composition of the peptide in G. lucidum was rich in phenylalanine, aspartic acid, proline, histidine, and isoleucine [ 11 ].…”

Advances in research on the active constituents and physiological effects of Ganoderma lucidum

“…It was found that protein from both the mycelia and fruit body exhibited antioxidant capacity. The mycelial protein extract showed better scavenging activities than those shown by fruiting body protein extract, in terms of both 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical-and 2,2-diphenylpicrylhydrazyl radical (DPPH•) radical-scavenging abilities (Sa-Ard et al 2015). Oxidative stress markers were measured by using the comet assay to measure ROS generation.…”

Current and future perspective on antimicrobial and anti-parasitic activities of Ganoderma sp.: an update

“…Literatures reveal most of the antibacterial tests are performed on crude extract with significant effective results rather than pure compounds (Sa-Ard et al 2015; Zengin et al 2015; Cilerdzic et al 2016). In addition, scanty information is available on the in vivo model test of effective compounds; we noticed only compounds ( 27 ) and 28 have been tested the in vivo model of the Methicillin-resistant Staphylococcus aureus (MRSA)-infected mouse (Mikolasch et al 2016).…”