Copper Binding Proteins in Spinach Tolerant to Excess Copper

Tukendorf, A; Lyszcz, S.; Baszyński, Tadeusz

doi:10.1016/s0176-1617(84)80034-6

Cited by 23 publications

(5 citation statements)

References 22 publications

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“…Pretreatment either with SA or AsA mitigated Cu-induced damage in cotton and restored root and shoot growth. Despite increasing overall Cu uptake, plant growth regulators, especially SA, enhanced cotton tolerance to Cu-induced stress possibly through promoting cellular detoxification of Cu (Tukendorf et al 1984), formation of complexes with phytochelatins (PCs), and its compartmentalization (Cobbett and Goldsbrough 2002;Daud et al 2009). A similar effect of SA and AsA in ameliorating metal-induced toxicity via restricting metal translocation to the root has previously been suggested by Yang et al (2003).…”

Section: Discussionmentioning

confidence: 65%

Pretreatment with salicylic acid and ascorbic acid significantly mitigate oxidative stress induced by copper in cotton genotypes

Mei

Daud

Ullah

et al. 2015

Environ Sci Pollut Res

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Higher uptake and translocation of copper (Cu) into plant tissues can cause serious physiological and biochemical alterations in root and leaf tissues of plants. The present study investigates the ameliorative role of salicylic acid (SA) and ascorbic acid (AsA) against Cu-induced toxicity changes in cotton genotypes (two parental lines (J208, Z905) and their hybrid line (ZD14)). To study the tolerance potential against Cu (100 μM) stress, 2-week-old cotton seedlings were pretreated with 100 μM either SA or AsA for three days. Elevated Cu concentration in nutrient media increased Cu accumulation in roots and shoots of all the three cotton genotypes studied. Roots were the main Cu storage site, followed by leaves and stems. Increased cellular Cu concentration significantly inhibited the root and shoot development, although leaf growth was more sensitive toward Cu toxicity. Cu-induced oxidative stress to cotton leaves was evident from significantly increased hydrogen peroxide (H2O2) contents and lipid membrane damage. Increasing Cu translocation toward cotton leaves strongly influenced the malondialdehyde (MDA) contents, which, in turn, inhibited biomass production. SA and AsA pretreated cotton seedlings showed better growth under Cu stress. Despite increase in overall Cu uptake, the SA-pretreated seedlings could defy Cu toxicity through inhibited Cu translocation and modification in the activities of antioxidative enzymes. Whereas, tolerance to Cu-induced toxicity in AsA pretreated plants was associated with Cu exclusion from tissues and reduction of the overall Cu uptake. The present study revealed that the alleviatory role of AsA was significantly higher than SA regarding Cu stress in our experimental cotton genotypes. Furthermore, the hybrid cotton genotype (ZD14) performed well followed by J208 and Z905 in the present experimental setup.

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Section: Discussionmentioning

confidence: 65%

Pretreatment with salicylic acid and ascorbic acid significantly mitigate oxidative stress induced by copper in cotton genotypes

Mei

Daud

Ullah

et al. 2015

Environ Sci Pollut Res

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“…A similar dependence has been noted for metallothioneins (22). We conclude that the molecular weight of the native metal-containing phytochelatin complex is 2000-4000 (23-26), rather than the %10,000 observed at low ionic strength (13,(15)(16)(17)(18)(19)(20)(21). We reexamined the heavy metal metabolism in S. cucubalus (19) and A. tenuis (23) cell cultures, as well as in plants of L. esculentum (26) and Z. mayst.…”

mentioning

confidence: 96%

Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins

Grill

Winnacker²,

Zenk

1987

Proc. Natl. Acad. Sci. U.S.A.

746

433

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Phytochelatins are a class of heavy-metalbinding peptides previously isolated from cell suspension cultures of several dicotyledonous and monocotyledonous plants. These peptides consist of repetitive y-glutamylcysteine units with a carboxyl-terminal glycine and range from 5 to 17 amino acids in length. In the present paper we show that all plants tested synthesized phytochelatins upon exposure to heavy metal ions. No evidence for the occurrence of metallothionein-like proteins was found. All data so far obtained indicate that phytochelatins are involved in detoxification and homeostasis of heavy metals in plants and thus serve functions analogous to those of metallothioneins in animals and some fungi. Phytochelatins are induced by a wide range of metal anions and cations. Phytochelatin synthesis in suspension cultures was inhibited by buthionine sulfoximine, a specific inhibitor of y-glutamylcysteine synthetase (EC 6.3.2.2). This finding and kinetic studies of phytochelatin induction point to a synthesis from glutathione or its precursor, r-glutamylcys-

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“…A low molecular weight protein (Mr 12000-13000 in the globulin and albumin fraction was found in a higher concentration. Tukendorf et al (1984) found that in the presence of excess copper, spinach formed copper complexes due to copper binding by preexisting proteins and their synthesis was stimulated by the excess copper. These results suggest that the protein content of the stem injected peanuts would be similar to "normal" peanuts and the bioavailability of copper would be unaffected by the labeling method.…”

Section: Resultsmentioning

confidence: 99%

Growth and intrinsic labeling of peanuts with copper-65 for use in human bioavailibility studies

Starks¹,

Johnson²

1985

J. Agric. Food Chem.

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Copper Binding Proteins in Spinach Tolerant to Excess Copper

Cited by 23 publications

References 22 publications

Pretreatment with salicylic acid and ascorbic acid significantly mitigate oxidative stress induced by copper in cotton genotypes

Pretreatment with salicylic acid and ascorbic acid significantly mitigate oxidative stress induced by copper in cotton genotypes

Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins

Growth and intrinsic labeling of peanuts with copper-65 for use in human bioavailibility studies

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