Copper-induced calcium release from ER involves the activation of ryanodine-sensitive and IP3-sensitive channels in<i>Ulva compressa</i>

González, Alberto; Trebotich, Jovanka; Vergara, Eva; Medina, Cristóbal; Morales, Bernardo; Moenne, Alejandra

doi:10.4161/psb.5.12.13977

Cited by 19 publications

(16 citation statements)

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“…In this sense, it is possible that the synthesis of cADPR and/or NAADP is required for the activation of ryanodine-and NAADP-sensitive channels allowing the intracellular calcium increase which, in turn, may promote the activation of VDCC located in the plasma membrane via CaMs, as it has been observed in neurons where the increase in intracellular calcium promoted the activation of non-selective cation channels in the plasma membrane 6 and in cardiac cells where the increase in intracellular calcium activates of L-type VDCC in the plasma membrane via CaMs. 23 Based on present results and previous work, 19,20 an integrative model of copper-induced cellular responses is presented (Fig. 4).…”

supporting

confidence: 52%

“…19 In addition, the depletion of calcium from ER using thapsigargin resulted in a complete inhibition of copper-induced calcium release indicating that ryanodine, Ins(1,4,5)P 3 -and NAADP-sensitive calcium channels are located in ER, as in terrestrial plants. 20 Moreover, the increase in intracellular calcium induced the synthesis of hydrogen peroxide and nitric oxide (NO) levels in mitochondria and chloroplasts and, in turn, hydrogen peroxide and NO activate calcium release from ER indicating a mutual influence (cross-talk) among these intracellular signals.…”

mentioning

confidence: 99%

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Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

González

Cabrera

Mellado

et al. 2012

Plant Signaling & Behavior

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The marine alga Ulva compressa exposed to 10 mM copper showed a triphasic increase of intracellular calcium with maximal levels at 2, 3 and 12 h involving the activation of ryanodine-, Ins(1,4,5)P 3 -and NAADP-sensitive calcium channels. In order to analyze the requirement of extracellular calcium entry for intracellular calcium release as well as the activation of voltage-dependent calcium channels (VDCC) and phospholipase C, U. compressa was treated with EGTA, a non-permeable calcium chelating agent, with verapamil, nipfedipine and diltiazem, inhibitors of L-type VDCC, and with neomycin and U731222, inhibitors of phospholipase C. The release of intracellular calcium was partially inhibited with EGTA at 2 and 3 h and completely inhibited at 12 h of copper exposure and decreased with inhibitors of L-type VDCC and phospholipase C. Thus, copper-induced intracellular calcium release depends on calcium entry and activation of L-type VDCC and phospholipase C. An integrative model of copper-induced cellular responses in U. compressa is presented.In animals and plants, physiological and pathological stimuli induced the release of intracellular calcium with characteristic spatiotemporal patterns which depends on the nature and intensity of the stimulus and the cell type.

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confidence: 52%

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confidence: 99%

Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

González

Cabrera

Mellado

et al. 2012

Plant Signaling & Behavior

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“…As mentioned before, it was previously determined that copper induced calcium release in U. compressa exclusively from ER, indicating that NAAD-, ryanodine-, and IP 3 -sentive channels are located in the ER (González et al, 2010a). In addition, we showed that copper-induced calcium release is activated by NO and H 2 O 2 , indicating a cross talk between these intracellular signals.…”

Section: Copper-induced H 2 O 2 Synthesis Is Dependent On Calcium Relmentioning

confidence: 62%

“…In this sense, it has been determined that NAADP-sensitive calcium channels are located in ER in terrestrial plants (Navazio et al, 2000), whereas they are found in lysosome-related acidic organelles in marine invertebrates and mammalian cells (Calcraft et al, 2009;Galione et al, 2010). In addition, we have previously shown that copper induced calcium release exclusively from ER in U. compressa (González et al, 2010a). Thus, NAADP-sensitive calcium channels in U. compressa might be located in ER as in terrestrial plants.…”

Section: Calcium Activates Antioxidant Protein Gene Expression Via Camentioning

confidence: 99%

“…In addition, it was shown that copper-induced intracellular calcium release originated exclusively in the endoplasmic reticulum (ER) and involved the activation of ryanodine-sensitive and inositol 1,4,5 triphosphate (IP 3 )-sensitive calcium channels (González et al, 2010a), and production of H 2 O 2 occurred exclusively in organelles (González et al, 2010b).…”

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Cross Talk among Calcium, Hydrogen Peroxide, and Nitric Oxide and Activation of Gene Expression Involving Calmodulins and Calcium-Dependent Protein Kinases in Ulva compressa Exposed to Copper Excess

et al. 2012

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To analyze the copper-induced cross talk among calcium, nitric oxide (NO), and hydrogen peroxide (H2O2) and the calcium-dependent activation of gene expression, the marine alga Ulva compressa was treated with the inhibitors of calcium channels, ned-19, ryanodine, and xestospongin C, of chloroplasts and mitochondrial electron transport chains, 3-(3,4-dichlorophenyl)-1,1-dimethylurea and antimycin A, of pyruvate dehydrogenase, moniliformin, of calmodulins, N-(6-aminohexyl)-5-chloro-1-naphtalene sulfonamide, and of calcium-dependent protein kinases, staurosporine, as well as with the scavengers of NO, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, and of H2O2, ascorbate, and exposed to a sublethal concentration of copper (10 μm) for 24 h. The level of NO increased at 2 and 12 h. The first peak was inhibited by ned-19 and 3-(2,3-dichlorophenyl)-1,1-dimethylurea and the second peak by ned-19 and antimycin A, indicating that NO synthesis is dependent on calcium release and occurs in organelles. The level of H2O2 increased at 2, 3, and 12 h and was inhibited by ned-19, ryanodine, xestospongin C, and moniliformin, indicating that H2O2 accumulation is dependent on calcium release and Krebs cycle activity. In addition, pyruvate dehydrogenase, 2-oxoxglutarate dehydrogenase, and isocitrate dehydrogenase activities of the Krebs cycle increased at 2, 3, 12, and/or 14 h, and these increases were inhibited in vitro by EGTA, a calcium chelating agent. Calcium release at 2, 3, and 12 h was inhibited by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and ascorbate, indicating activation by NO and H2O2. In addition, the level of antioxidant protein gene transcripts decreased with N-(6-aminohexyl)-5-chloro-1-naphtalene sulfonamide and staurosporine. Thus, there is a copper-induced cross talk among calcium, H2O2, and NO and a calcium-dependent activation of gene expression involving calmodulins and calcium-dependent protein kinases.

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Microalgae‐Derived Health Supplements to Therapeutic Shifts: Redox‐Based Study Opportunities with AIE‐Based Technologies

Reza

Zhu

Qin

et al. 2021

Adv Healthcare Materials

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Reactive oxygen species (ROS) are highly reactive molecules, serve the normal signaling in different cell types. Targeting ROS as the chemical signals, different stress based strategies have been developed to synthesis different anti‐inflammatory molecules in microalgae. These molecules could be utilized as health supplements in human. To provoke the ROS‐mediated defence systems, their connotation with the associated conditions must be well understood, therefore, proper tools for studying ROS in natural state are essential. The in vivo detection of ROS with phosphorescent probes offers promising opportunities to study these molecules in a non‐invasive manner. Most of the common problems in the traditional fluorescent probes are lower photostability, excitation intensity, slow responsiveness, and the microenvironment that challenge their performance. Some ROS‐specific aggregationinduced emission luminogens (AIEgens) with pronounced spatial and temporal resolution have recently demonstrated high selectivity, rapid responsiveness, and efficacies to resolve the aggregation‐caused quenching issues. The nanocomposites of some AIE‐photosensitizers can also improve the ROS‐mediated photodynamic therapy. These AIEgens could be used to induce bioactive components in microalgae through altering the ROS signaling, therefore are more auspicious for biomedical research. This study reviews the prospects of AIEgen‐based technologies to understand the ROS mediated bio‐physiological processes in microalgae for better healthcare benefits.

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Copper-induced calcium release from ER involves the activation of ryanodine-sensitive and IP3-sensitive channels inUlva compressa

Cited by 19 publications

References 19 publications

Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

Cross Talk among Calcium, Hydrogen Peroxide, and Nitric Oxide and Activation of Gene Expression Involving Calmodulins and Calcium-Dependent Protein Kinases in Ulva compressa Exposed to Copper Excess

Microalgae‐Derived Health Supplements to Therapeutic Shifts: Redox‐Based Study Opportunities with AIE‐Based Technologies

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