Copper-induced changes in nutrient uptake, enzymatic and non-enzymatic antioxidant systems in horehound ( Marrubium vulgare  L.)

Amri, Bédis; Kaab, Sofiene Ben; Gouia, Houda; Martino, Emanuela; Collina, Simona; Kaâb, Leila Bettaeib Ben

doi:10.17129/botsci.778

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…As a first screening, we performed the DPPH assay, commonly used to determine FRS activity of natural compounds [ 45 ]. Moreover, this assay can be helpful in lead-finding of novel antioxidants.…”

Section: Resultsmentioning

confidence: 99%

Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds

Pellavio

Rui

Caliogna

et al. 2017

IJMS

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Some aquaporins (AQPs) have been recently demonstrated to facilitate the diffusion of hydrogen peroxide (H2O2) from the producing cells to the extracellular fluid, and their reactive oxygen species scavenging properties have been defined. Nevertheless, the identification of different AQPs acting as peroxiporins, their functional role in eustress and distress, and the identification of antioxidant compounds able to regulate AQP gating, remain unsolved. This study aims to investigate, in HeLa cells: (1) the expression of different AQPs; (2) the evaluation of naringenin, quercetin, (R)-aloesaponol III 8-methyl ether, marrubiin, and curcumin antioxidant profiles, via α,α-diphenyl-β-picrylhydrazyl assay; (3) the effect of the compounds on the water permeability in the presence and in the absence of oxidative stress; and (4) the effect of pre- and post-treatment with the compounds on the H2O2 content in heat-stressed cells. Results showed that HeLa cells expressed AQP1, 3, 8, and 11 proteins. The oxidative stress reduced the water transport, and both pre- and post-treatment with the natural compounds recovering the water permeability, with the exception of curcumin. Moreover, the pre- and post-treatment with all the compounds reduced the H2O2 content of heat-stressed cells. This study confirms that oxidative stress reduced water AQP-mediated permeability, reversed by some chemical antioxidant compounds. Moreover, curcumin was shown to regulate AQP gating. This suggests a novel mechanism to regulate cell signaling and survival during stress, and to manipulate key signaling pathways in cancer and degenerative diseases.

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Section: Resultsmentioning

confidence: 99%

Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds

Pellavio

Rui

Caliogna

et al. 2017

IJMS

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“…M. vulgare prefers alkaline soils [ 10 ]. Amri et al [ 11 ] reported that different concentrations of copper reduce the uptake and translocation of cationic elements Fe 2+ , K + , and Ca 2+ in a Cu 2+ concentration-dependent manner in the aboveground part of the plant. Treatment with copper stimulates antioxidant enzymes activities (superoxide dismutase (SOD) and catalase (CAT) enzymes) and increases the total phenolics (flavonoid) content.…”

Section: Botanical and Agronomical Featuresmentioning

confidence: 99%

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

et al. 2020

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Marrubium vulgare is a plant with high bioactive potential. It contains marrubiin, a labdane diterpene that is characteristic for this genus, as well as a complex mixture of phenolic compounds. According to numerous studies, M. vulgare acts as a good antioxidant agent, and due to this, it could potentially be useful in treatments of cancer, diabetes mellitus, and liver diseases. In addition, its anti-inflammatory, wound-healing, antihypertensive, hypolipidemic, and sedative potential are discussed. Apart from that, its antimicrobial activity, especially against Gram+ bacteria, fungi, herpes simplex virus, and parasites such as Toxoplasma gondii, Trichomonas vaginalis, and Plasmodium berghei-berghei was recorded. Additionally, it could be used as a chicken lice repellent, herbicide, and natural insecticide against mosquito larvae and natural molluscicide. In veterinary medicine, M. vulgare can be used as an anthelmintic against the eggs and larvae of bovine strongyles parasites, and as an antibiotic against bovine mastitis caused by resistant bacterial strains. Due to the mentioned benefits, there is a tendency for the cultivation of M. vulgare in order to ensure high-quality raw material, but more firm scientific evidence and well-designed clinical trials are necessary for the well-established use of M. vulgare herb and its preparations.

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“…Our results showed that raw materials originating from unstressed plant contained more marrubiin compared to leaves harvested from plants grown in stressed conditions. In detail, the plants were treated with CuSO 4 (80, 200 and 300 mg/L) for eight weeks according to the related literature . As highlighted in Figure , there are significant differences ( P < 0.05) in the amount of marrubiin, depending on the growth conditions.…”

Section: Resultsmentioning

confidence: 99%

The use of a microwave‐assisted solvent extraction coupled with HPLC‐UV/PAD to assess the quality of Marrubium vulgareL. (white horehound) herbal raw material

Martino

Volpe

Cavalloro

et al. 2019

Phytochemical Analysis

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Introduction Marrubium vulgare is a herbal remedy presents in several European Pharmacopoeias and commonly marketed as white horehound. The chemotaxonomic marker of Marrubium genus is marrubiin and its content may change in response to biotic and abiotic stress. Objective Development of a microwave‐assisted solvent extraction (MASE) methodology suitable for exhaustively extracting marrubiin from M. vulgare leaves, easily applicable to large sets of samples. Evaluation of the influence of copper(II) on marrubiin production. Material and methods M. vulgare leaves were dried, extracted exploiting MASE and analysed via high‐performance liquid chromatography ultraviolet photodiode array detection (HPLC‐UV/PAD) system. A design of experiments approach was adopted to select the best extraction conditions. Extraction parameters (solvent composition, extraction time and temperature), were studied applying two full factorial experimental designs in a sequential approach. To analyse samples, a rapid HPLC‐UV/PAD method was set up. Results The best results in terms of marrubiin extraction yield were obtained extracting samples at 120°C with 100% ethanol, for 15 min (3 × 5 min microwave cycles). The developed methodology was successfully applied to matrices grown in Greenhouse conditions and under stress induced by copper(II), selected as model agent for abiotic stress. Progressively decreasing production of marrubiin was evidenced in connection with treatment with 80, 200 and 300 mg/L copper sulphate. Conclusion An efficient methodology for the extraction and determination of the amount of marrubiin in large sets of samples of M. vulgare plants was developed. Results demonstrated that marrubiin is an easily detectable marker useful for evaluating M. vulgare reaction to stress.

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Copper-induced changes in nutrient uptake, enzymatic and non-enzymatic antioxidant systems in horehound ( Marrubium vulgare L.)

Cited by 8 publications

References 22 publications

Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds

Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

The use of a microwave‐assisted solvent extraction coupled with HPLC‐UV/PAD to assess the quality of Marrubium vulgareL. (white horehound) herbal raw material

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