Copper induces apoptotic cell death through reactive oxygen species-triggered oxidative stress in the intertidal copepod Tigriopus japonicus

Rhee, Jae-Sung; Yu, In Tag; Kim, Bomi; Jeong, Chang‐Bum; Lee, Kyun-Woo; Kim, Minjung; Lee, Su‐Jae; Park, Gyung Soo; Lee, Jae‐Seong

doi:10.1016/j.aquatox.2013.02.013

Cited by 95 publications

(30 citation statements)

References 50 publications

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“…E.g., cadmium-induced apoptosis was reported in rat testes (Xu et al, 1996), mouse liver (Habeebu et al, 1998), rat lung epithelial cells (Hart et al, 1999), CL-3 human lung carcinoma cells (Chuang et al, 2000), HeLa human cervix carcinoma cells (Szuster-Ciesielska et al, 2000) and Rat-1 fibroblast cells (Kim et al, 2000). Copper, for its part, can induce an upregulation of apoptosis and related genes in zebrafish (Luzio et al, 2013) or induces the apoptotic cell death in the copepod Tigriopus japonicus (Rhee et al, 2013). Thus in order to assess the phenomenon of apoptosis we examined the integrity of the DNA of leukocytes.…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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a b s t r a c tThe immunotoxic effects of heavy metals on blood leukocytes of sea bass (Dicentrarchus labrax) were examined. The cells, separated by a discontinuous Percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10 À 7 M, 10 À 5 M, and 10 À 3 M) and their immunotoxic effect was then evaluated by measuring neutral red uptake, MTT assay, DNA fragmentation and Hsp70 gene expression. First of all, we demonstrated that the cells treated in vitro could incorporate Cd and Cu. A relationship between heavy metal exposure and dose-time-dependent alterations in responses of leukocytes from blood was found for both metals, but copper was more immunotoxic than cadmium in all assays performed. A significant reduction in the cells' ability to uptake neutral red and viability by MTT assay was recorded, indicating that both cadmium and copper could change the membrane permeability, inducing cellular apoptosis when the concentration of metals reached 10 À 3 M. The apoptotic effect may also explain the high level of cytotoxicity found when the leukocytes were exposed to higher concentration of metals. These results demonstrated that toxic effect of copper and cadmium affect on the mechanisms of cellmediated immunity reducing the immune defences of the organism.

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Section: Discussionmentioning

confidence: 99%

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Vazzana¹,

Celi²,

Tramati³

et al. 2014

Ecotoxicology and Environmental Safety

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“…Gill reactive oxygen species (ROS) level was measured using 2 0 7 0 -dichlorodihydrofluorescein diacetate which was oxidized to fluorescent dichlorofluorescein (DCF) by ROS. The measurements were obtained with an excitation wavelength at 485 nm and emission wavelength at 520 nm with fluorescence spectroscopy, and normalized by total protein and represented as a percentage of fluorescence of DCF, according to Rhee et al [47]. The contents of malondialdehyde (MDA), protein carbonyl (PC) and reduced glutathione (GSH) in the gill were determined according to the procedures described by Zhang et al [48], Baltacıoglu et al [49], and Vardi et al [50], respectively.…”

Section: Biochemical Analysismentioning

confidence: 99%

Dietary riboflavin deficiency decreases immunity and antioxidant capacity, and changes tight junction proteins and related signaling molecules mRNA expression in the gills of young grass carp (Ctenopharyngodon idella)

Chen

Feng

Jiang

et al. 2015

Fish & Shellfish Immunology

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“…In the copepod T. japonicus, p38 MAPK has been shown to be activated in response to ultraviolet B (UVB) radiation and copper exposure. 36,37 In sea urchins, p38 MAPK has also been shown to be activated by titanium dioxide nanoparticles. 38 Thus, the strong activation of p-JNK and p-p38 MAPK in rotifers exposed to 0.05-μm microbeads suggests that these two MAPK-activating proteins are involved in the subsequent signal transduction that generates the oxidative stress response.…”

Section: Environmental Science and Technologymentioning

confidence: 99%

Microplastic Size-Dependent Toxicity, Oxidative Stress Induction, and p-JNK and p-p38 Activation in the Monogonont Rotifer (Brachionus koreanus)

Jeong

Won

Kang

et al. 2016

Environ. Sci. Technol.

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1,010

341

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In this study, we evaluated accumulation and adverse effects of ingestion of microplastics in the monogonont rotifer (Brachionus koreanus). The dependence of microplastic toxicity on particle size was investigated by measuring several in vivo end points and studying the ingestion and egestion using 0.05-, 0.5-, and 6-μm nonfunctionalized polystyrene microbeads. To identify the defense mechanisms activated in response to microplastic exposure, the activities of several antioxidant-related enzymes and the phosphorylation status of mitogen-activated protein kinases (MAPKs) were determined. Exposure to polystyrene microbeads of all sizes led to significant sizedependent effects, including reduced growth rate, reduced fecundity, decreased lifespan and longer reproduction time. Rotifers exposed to 6-μm fluorescently labeled microbeads exhibited almost no fluorescence after 24 h, while rotifers exposed to 0.05-and 0.5-μm fluorescently labeled microbeads displayed fluorescence until 48 h, suggesting that 6-μm microbeads are more effectively egested from B. koreanus than 0.05-or 0.5-μm microbeads. This observation provides a potential explanation for our findings that microbead toxicity was sizedependent and smaller microbeads were more toxic. In vitro tests revealed that antioxidant-related enzymes and MAPK signaling pathways were significantly activated in response to microplastic exposure in a size-dependent manner.

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Copper induces apoptotic cell death through reactive oxygen species-triggered oxidative stress in the intertidal copepod Tigriopus japonicus

Cited by 95 publications

References 50 publications

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

In vitro effect of cadmium and copper on separated blood leukocytes of Dicentrarchus labrax

Dietary riboflavin deficiency decreases immunity and antioxidant capacity, and changes tight junction proteins and related signaling molecules mRNA expression in the gills of young grass carp (Ctenopharyngodon idella)

Microplastic Size-Dependent Toxicity, Oxidative Stress Induction, and p-JNK and p-p38 Activation in the Monogonont Rotifer (Brachionus koreanus)

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